18:1 Liss Rhod PE  (Synonyms: 18:1 Lissamine rhodamine PE)

18:1 Liss Rhod (18:1 Lissamine rhodamine) PE is a fluorescent phospholipid and fluorescent probe.18:1 Liss Rhod PE admixes into phospholipid inks for large-scale monitoring of dip-pen nanolithography-generated lithographic structures via fluorescence microscopy.18:1 Liss Rhod PE undergoes phase separation or self-quenching under certain conditions in thin lipid membrane stacks^[1].

18:1 Liss Rhod PE is a dedicated fluorescent probe for dip-pen nanolithography (DPN) phospholipid patterning research. 18:1 Liss Rhod PE (1 mol %) exhibits decreased fluorescence intensity in the third bilayer height layer of DPN-patterned DOPC lipid stacks blended with 20 mol % DNP Cap PE, which may result from phase separation-induced local changes in probe concentration^[1].
18:1 Liss Rhod PE is incorporated into phospholipid ink as a fluorescent label to enable large-scale, high-throughput microscopic imaging of stacked phospholipid membrane patterns prepared by DPN^[1].
1. Preparation of Reagents, Equipment and Consumables
Main reagents: 18:1 Liss Rhod PE, carrier phospholipid DOPC, functional phospholipid DNP Cap PE.
Solvent: HPLC-grade chloroform.
Lithography consumables: Inkwell chip, M-2 pen array (both products of NanoInk, Inc.); hydrophilic standard SURF sample (silica oxide substrate with anti-reflective coating, from Nanolane).
Core equipment: DPN 5000 system (NanoInk, Inc.), Nikon Eclipse 80i fluorescence microscope.
2. Preparation of DPN Ink Containing Fluorescent Probe
Preparation of basic stock solution: Prepare a 1 mg/mL DOPC solution using HPLC-grade chloroform as the solvent.
Incorporation of fluorescent probe: Add 18:1 Liss Rhod PE at a fixed molar ratio of 1 mol% to the above DOPC chloroform stock solution, and simultaneously add 0/10/20 mol% of DNP Cap PE according to experimental requirements. Mix thoroughly to obtain DPN lithography ink.
3. Coating of DPN Pen Array and Preparation of Patterns
Ink loading: Add 3 μL of the prepared phospholipid ink into the Inkwell chip.
Pen array coating: Coat the M-2 pen array with the loaded ink under an environment of 70% relative humidity, with a fixed coating duration of 10 min.
Substrate patterning: Fabricate phospholipid patterns on the hydrophilic SURF substrate via the DPN 5000 system under conditions of 48% relative humidity and 24°C room temperature, to obtain stacked lipid membrane structures.
4. Fluorescence Microscopy Monitoring and Quality Control
Imaging screening: Perform fluorescence imaging on the lithographed samples using a Nikon Eclipse 80i fluorescence microscope to obtain information on the overall morphology, size and integrity of lipid patterns, and complete large-area, high-throughput quality screening.
Stability verification: Conduct fluorescence microscopy detection on the same sample both before and after AFM characterization to verify the structural stability of lipid patterns prepared by DPN during the characterization process.
18:1 Liss Rhod PE enables quantitative correlation between the physical height of phospholipid membrane stacks prepared by DPN and the number of membrane layers, achieving rapid characterization of three-dimensional topological information of lipid membrane stacking structures^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1301.72

C₆₈H₁₀₉N₄O₁₄PS₂

384833-00-5

Solid

Purple to purplish red

O=S([O-])(C1=C(C=CC(S(NCCOP(OC[C@H](OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O)([O-])=O)(=O)=O)=C1)C2=C(C=CC(N(CC)CC)=C3)C3=[O+]C4=C2C=CC(N(CC)CC)=C4)=O.[NH4+]

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Hirtz M, et al. Comparative height measurements of dip-pen nanolithography-produced lipid membrane stacks with atomic force, fluorescence, and surface-enhanced ellipsometric contrast microscopy. Langmuir. 2011;27(18):11605-11608.  [Content Brief]