1. Metabolic Enzyme/Protease
    Protein Tyrosine Kinase/RTK
  2. E1/E2/E3 Enzyme
    TAM Receptor
  3. 2-D08

2-D08 

Cat. No.: HY-114166 Purity: 99.04%
Handling Instructions

2-D08 is a cell permeable, mechanistically unique inhibitor of protein SUMOylation. 2-D08 also inhibits Axl with an IC50 of 0.49 nM.

For research use only. We do not sell to patients.

2-D08 Chemical Structure

2-D08 Chemical Structure

CAS No. : 144707-18-6

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 100 In-stock
Estimated Time of Arrival: December 31
25 mg USD 210 In-stock
Estimated Time of Arrival: December 31
50 mg USD 340 In-stock
Estimated Time of Arrival: December 31
100 mg USD 590 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE 2-D08

    2-D08 purchased from MCE. Usage Cited in: Life Sci. 2020 May 28;117859.

    The expression levels of TGF-β/SMAD and HIF-1 signaling pathways-related proteins, including Col III, Smad2, Smad3, Smad4 and HIF-1α are measured by western blotting in HFFs and HKFs. The band intensity ratio of the TGF-β/SMAD and HIF-1 signaling related protein to reference protein is significantly decreased in the 2-D08 group.
    • Biological Activity

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    Description

    2-D08 is a cell permeable, mechanistically unique inhibitor of protein SUMOylation. 2-D08 also inhibits Axl with an IC50 of 0.49 nM.

    IC50 & Target

    IC50: 0.49 nM (Axl)[2]

    In Vitro

    2-D08 inhibits sumoylation by preventing transfer of SUMO from the UBC9-SUMO thioester to the substrate[1]. 2-D08 decreases the ratio of p-Axl to t-Axl in a dose-dependent manner. Suppression of Axl kinase activity by 2D08 disrupts the cytoskeleton and actin filaments with re-organization at cellular junctions[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    270.24

    Formula

    C₁₅H₁₀O₅

    CAS No.

    144707-18-6

    SMILES

    O=C1C=C(C2=CC=C(O)C(O)=C2O)OC3=CC=CC=C13

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 150 mg/mL (555.06 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7004 mL 18.5021 mL 37.0041 mL
    5 mM 0.7401 mL 3.7004 mL 7.4008 mL
    10 mM 0.3700 mL 1.8502 mL 3.7004 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (9.25 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [2]

    Human lung multi-potent cells at passage 5 are incubated with DMEM+0.5% BSA+penicillin/streptomycin containing either 0.1% DMSO (vehicle) or 2D08 (10 μM) on Permanox culture slides for 6 days. Cells are fixed with 4% PFA for 30 min and then blocked and permeabilized with 10% goat serum and 0.3% Triton-X 100 in PBS for 30 min[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Mice[3]

    Eight- to 10-week-old mice (both male and female) are used for the study. A 5 cm-long glass pipette is buffered with Mineral Oil and then attached to the injection apparatus to take up 10 μL of 2-D08 (30 μM) or NaCl[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    2-D08E1/E2/E3 EnzymeTAM ReceptorE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseTyro3AxlMerInhibitorinhibitorinhibit

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    Product Name:
    2-D08
    Cat. No.:
    HY-114166
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