3x DYKDDDDK Tag TFA

Name: 3x DYKDDDDK Tag TFA
Brand: MedChemExpress
SKU: HY-P3332A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P3332A Purity: 98.17%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance .

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Custom Peptide Synthesis

3x DYKDDDDK Tag TFA 화학구조

사이즈	재고	수량
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of 3x DYKDDDDK Tag TFA:

3x DYKDDDDK Tag In-stock

1 Publications Citing Use of MCE 3x DYKDDDDK Tag TFA

•Int Immunopharmacol. 2026 May 1:176:116468. [Abstract]

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•Related Small Molecules:

•Related Proteins:

Biological Activity
순도&문서
References
고객리뷰

제품 설명

In Vitro

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

3116.90

화학식

C₁₂₅H₁₇₇F₃N₃₀O₆₀

Appearance

Solid

Color

White to off-white

Sequence Shortening

DYKDDDDKDYKDDDDKDYKDDDDK

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

용액&용해도

In Vitro:

H₂O : ≥ 100 mg/mL (32.08 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.3208 mL	1.6042 mL	3.2083 mL
5 mM	0.0642 mL	0.3208 mL	0.6417 mL
10 mM	0.0321 mL	0.1604 mL	0.3208 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 98.17%

Select Batch:

Data Sheet (269 KB) SDS (252 KB)

COA (242 KB) RP-HPLC (259 KB) MS (200 KB) Elemental Analysis Report (100 KB)

Handling Instructions (2659 KB)

References

[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830. [Content Brief]

[2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.3208 mL	1.6042 mL	3.2083 mL	8.0208 mL
	5 mM	0.0642 mL	0.3208 mL	0.6417 mL	1.6042 mL
	10 mM	0.0321 mL	0.1604 mL	0.3208 mL	0.8021 mL
	15 mM	0.0214 mL	0.1069 mL	0.2139 mL	0.5347 mL
	20 mM	0.0160 mL	0.0802 mL	0.1604 mL	0.4010 mL
	25 mM	0.0128 mL	0.0642 mL	0.1283 mL	0.3208 mL
	30 mM	0.0107 mL	0.0535 mL	0.1069 mL	0.2674 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.