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  2. 3x DYKDDDDK Tag

3x DYKDDDDK Tag 

Cat. No.: HY-P3332
Handling Instructions

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance .

For research use only. We do not sell to patients.

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3x DYKDDDDK Tag Chemical Structure

3x DYKDDDDK Tag Chemical Structure

CAS No. : 921765-76-6

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Description

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance [1][2].

In Vitro

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

3002.88

Formula

C₁₂₃H₁₇₆N₃₀O₅₈

CAS No.
Sequence Shortening

DYKDDDDKDYKDDDDKDYKDDDDK

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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3x DYKDDDDK Tag
Cat. No.:
HY-P3332
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