3x DYKDDDDK Tag TFA

Name: 3x DYKDDDDK Tag TFA
Brand: MedChemExpress
SKU: HY-P3332A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P3332A Purity: 98.10%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance .

For research use only. We do not sell to patients.

Custom Peptide Synthesis

3x DYKDDDDK Tag TFA Chemical Structure

Size	Price	Stock	Quantity
1 mg	USD 75	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of 3x DYKDDDDK Tag TFA:

3x DYKDDDDK Tag In-stock

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

3116.90

Formula

C₁₂₅H₁₇₇F₃N₃₀O₆₀

Appearance

Solid

Color

White to off-white

Sequence Shortening

DYKDDDDKDYKDDDDKDYKDDDDK

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (32.08 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.3208 mL	1.6042 mL	3.2083 mL
5 mM	0.0642 mL	0.3208 mL	0.6417 mL
10 mM	0.0321 mL	0.1604 mL	0.3208 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.10%

Select Batch:

Data Sheet (268 KB) SDS (251 KB)

COA (242 KB) RP-HPLC (210 KB) MS (200 KB) Elemental Analysis Report (101 KB)

Handling Instructions (2659 KB)

References

[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830. [Content Brief]

[2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	0.3208 mL	1.6042 mL	3.2083 mL	8.0208 mL
	5 mM	0.0642 mL	0.3208 mL	0.6417 mL	1.6042 mL
	10 mM	0.0321 mL	0.1604 mL	0.3208 mL	0.8021 mL
	15 mM	0.0214 mL	0.1069 mL	0.2139 mL	0.5347 mL
	20 mM	0.0160 mL	0.0802 mL	0.1604 mL	0.4010 mL
	25 mM	0.0128 mL	0.0642 mL	0.1283 mL	0.3208 mL
	30 mM	0.0107 mL	0.0535 mL	0.1069 mL	0.2674 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3x DYKDDDDK TagOthersInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

3x DYKDDDDK Tag TFA

Customer Review

Other Forms of 3x DYKDDDDK Tag TFA: