4-Azido-L-phenylalanine hydrochloride (Synonyms: p-Azidophenylalanine hydrochloride; p-Azido-L-phenylalanine hydrochloride)

Name: 4-Azido-L-phenylalanine hydrochloride
Brand: MedChemExpress
SKU: HY-16714A
Rating: 5.0 (9 reviews)

Cat. No.: HY-16714A Purity: 99.88%: Data Sheet
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4-Azido-L-phenylalanine hydrochloride is an unnatural amino acid, which is used as an effective vibrational reporter of local protein environments. 4-Azido-L-phenylalanine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

CAS No. : 34670-43-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 9 publication(s) in Google Scholar

Other Forms of 4-Azido-L-phenylalanine hydrochloride:

4-Azido-L-phenylalanine In-stock

9 Publications Citing Use of MCE 4-Azido-L-phenylalanine hydrochloride

Bio/Physico-chemical Assay

: 4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]; 4-Azido-L-phenylalanine (Azi) (1 mM). The expression of mutated VSV-G proteins (mProteins) in HEK 293T cells was detected by Western blot.

: 4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]; The site-specific display of 4-Azido-L-phenylalanine (Azi) (1 mM) at site 192 in mProtein D192, as a typical site, was verified by LC-MS/MS spectrum of a peptide derived from the purified protein after trypsinization.

: 4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]; 4-Azido-L-phenylalanine (Azi) (1 mM). The membrane proteins of mBiosomes were extracted and analyzed by Western blot.

: 4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]; The introduction of 4-Azido-L-phenylalanine (Azi) (1 mM) to site 246 caused evident changes in the secondary structures of the surrounding residues in mProtein A246.

: 4-Azido-L-phenylalanine hydrochloride purchased from MedChemExpress. Usage Cited in: Nanomicro Lett. 2023 Aug 12;15(1):197. [Abstract]; 4-Azido-L-phenylalanine (Azi) (1 mM). The proportion % of the secondary structures, including β-turn, helix, and random coil at surrounding sites (sites 245, 246, 247 and 248) in unmutated VSV-G, mProtein A246 and mProtein A247.

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Description

In Vitro

In cell-free protein synthesis (CFPS) method, 0.9-1.7 mg/mL of modified soluble super-folder green fluorescent protein (sfGFP) containing either 4-Azido-L-phenylalanine or p-propargyloxy-l-phenylalanine (pPaF) accumulate in the CFPS solutions^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

242.66

Formula

C₉H₁₁ClN₄O₂

CAS No.

34670-43-4

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(O)[C@@H](N)CC1=CC=C(N=[N+]=[N-])C=C1.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (412.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (41.21 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1210 mL	20.6050 mL	41.2099 mL
5 mM	0.8242 mL	4.1210 mL	8.2420 mL
10 mM	0.4121 mL	2.0605 mL	4.1210 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.30 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (68.70 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (275 KB) SDS (617 KB)

COA (251 KB) LCMS (264 KB)

Handling Instructions (2659 KB)

References

[1]. Albayrak C, et al. Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation. Nucleic Acids Res. 2013 Jun;41(11):5949-63. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.1210 mL	20.6050 mL	41.2099 mL	103.0248 mL
	5 mM	0.8242 mL	4.1210 mL	8.2420 mL	20.6050 mL
	10 mM	0.4121 mL	2.0605 mL	4.1210 mL	10.3025 mL
	15 mM	0.2747 mL	1.3737 mL	2.7473 mL	6.8683 mL
	20 mM	0.2060 mL	1.0302 mL	2.0605 mL	5.1512 mL
	25 mM	0.1648 mL	0.8242 mL	1.6484 mL	4.1210 mL
	30 mM	0.1374 mL	0.6868 mL	1.3737 mL	3.4342 mL
	40 mM	0.1030 mL	0.5151 mL	1.0302 mL	2.5756 mL
DMSO	50 mM	0.0824 mL	0.4121 mL	0.8242 mL	2.0605 mL
	60 mM	0.0687 mL	0.3434 mL	0.6868 mL	1.7171 mL
	80 mM	0.0515 mL	0.2576 mL	0.5151 mL	1.2878 mL
	100 mM	0.0412 mL	0.2060 mL	0.4121 mL	1.0302 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

4-Azido-L-phenylalanine hydrochloride Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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4-Azido-L-phenylalanine hydrochloride (Synonyms: p-Azidophenylalanine hydrochloride; p-Azido-L-phenylalanine hydrochloride)

Customer Review

Other Forms of 4-Azido-L-phenylalanine hydrochloride:

4-Azido-L-phenylalanine hydrochloride Related Antibodies

9 Publications Citing Use of MCE 4-Azido-L-phenylalanine hydrochloride

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

4-Azido-L-phenylalanine hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

4-Azido-L-phenylalanine hydrochloride Related Classifications

Keywords:

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Customer Review

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