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6-FAM  (Synonyms: 6-Carboxyfluorescein)

Cat. No.: HY-66021 Purity: 99.72%
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6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein and is mainly used for sequencing and labeling of nucleic acids.

For research use only. We do not sell to patients.

CAS No. : 3301-79-9

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Based on 2 publication(s) in Google Scholar

Other Forms of 6-FAM:

6-FAM Related Antibodies

Top Publications Citing Use of Products

    6-FAM purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 30;16(1):8124.  [Abstract]

    Concentration dependence of 6-FAM (6-CF, 100-600 μM) transport by human SLCO2A1. The results showed that the Michaelis constant (Km) of SLCO2A1 for 6-FAM (6-CF) was 84.10 ± 31.83 μM at pH 6.0.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein and is mainly used for sequencing and labeling of nucleic acids.

    In Vitro

    Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
    In cell experiments, oligonucleotide probes labeled with 6-carboxyfluorescein (6-FAM) were used, mainly through the fluorescence in situ hybridization (FISH) technique [1]. (1) Sample preparation: Prepare chromosome samples of chicken embryonic cells, including steps such as colchicine treatment, hypotonic swelling, and methanol-acetic acid fixation.
    (2) Pre-treatment: The chromosomes were treated with RNase A, pepsin and formaldehyde.
    (3) Hybridization: The 6-FAM-labeled oligonucleotide was dissolved in the hybridization buffer containing Escherichia coli tRNA (50% formamide, 2×SSC, 10% dextran sulfate) at a concentration of 20 ng/μL. After the DNA and the probe were denatured together, they were hybridized at room temperature in a humid box overnight.
    (4) Washing and re-staining: The hybridized slides were washed three times successively in 2×SSC, then dehydrated using a series of 70%-96% ethanol, dried, and sealed with an anti-quenching agent containing DAP I.
    (5) Observation and Analysis: Use the Leica DM4000B fluorescence microscope equipped with a monochrome CCD camera DFC350FX and appropriate filters to observe the samples. Then, use the QFISH software to capture and process the colored images of the chromosomes.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    6-FAM Related Antibodies
    Molecular Weight

    376.32

    Formula

    C21H12O7
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    Emission (Em)

    523
    Excitation (Ex)

    489
    SMILES

    O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC(C(O)=O)=C5
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 40 mg/mL (106.29 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6573 mL 13.2866 mL 26.5731 mL
    5 mM 0.5315 mL 2.6573 mL 5.3146 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.72%

    SDS (393 KB)

    COA (256 KB) HNMR (99 KB) LCMS (102 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6573 mL 13.2866 mL 26.5731 mL 66.4328 mL
    5 mM 0.5315 mL 2.6573 mL 5.3146 mL 13.2866 mL
    10 mM 0.2657 mL 1.3287 mL 2.6573 mL 6.6433 mL
    15 mM 0.1772 mL 0.8858 mL 1.7715 mL 4.4289 mL
    20 mM 0.1329 mL 0.6643 mL 1.3287 mL 3.3216 mL
    25 mM 0.1063 mL 0.5315 mL 1.0629 mL 2.6573 mL
    30 mM 0.0886 mL 0.4429 mL 0.8858 mL 2.2144 mL
    40 mM 0.0664 mL 0.3322 mL 0.6643 mL 1.6608 mL
    50 mM 0.0531 mL 0.2657 mL 0.5315 mL 1.3287 mL
    60 mM 0.0443 mL 0.2214 mL 0.4429 mL 1.1072 mL
    80 mM 0.0332 mL 0.1661 mL 0.3322 mL 0.8304 mL
    100 mM 0.0266 mL 0.1329 mL 0.2657 mL 0.6643 mL
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    6-FAM Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    6-FAM3301-79-96-CarboxyfluoresceinFluorescent DyeInhibitorinhibitorinhibit

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