ABTS (diammonium salt) (Synonyms: AzBTS-(NH4)2)

Name: ABTS diammonium salt
Brand: MedChemExpress
SKU: HY-15902
Rating: 5.0 (13 reviews)

製品番号: HY-15902 純度: 99.99%: Data Sheet SDS COA 取扱説明書
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ABTS diammonium salt (AzBTS-(NH4)2) is a substrate for horseradish peroxidase (HRP) conjugate. ABTS diammonium salt can be used to evaluate antioxidant capacity.

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CAS 番号 : 30931-67-0

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	在庫あり	Estimated Time of Arrival: December 31
Solid
500 mg	$50	在庫あり	Estimated Time of Arrival: December 31
1 g	$60	在庫あり	Estimated Time of Arrival: December 31
5 g		お問い合わせ
10 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 13 publication(s) in Google Scholar

顧客検証

ELISA

: ABTS diammonium salt purchased from MedChemExpress. Usage Cited in: Foods. 2026 Jan 22;15(2):408. [Abstract]; The ABTS·⁺ (a radical cation was generated by incubating a ABTS stock solution (7 mM) with potassium persulfate (2.45 mM) for 15 h in the dark) radical scavenging activities of GELNs (n = 3).

: ABTS diammonium salt purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Oct 24;22(1):653. [Abstract]; Scavenging ability of Cur against reactive oxygen species measured at different concentrations and times through the ABTS⁺ (a stable radical cation generated by oxidizing ABTS diammonium salt (5 mM) with manganese dioxide) radical scavenging assay.

: ABTS diammonium salt purchased from MedChemExpress. Usage Cited in: Molecules. 2024 Mar 29;29(7):1534.; Clearance of ABTS⁺ (a stock by mixing 5 mL volume of ABTS (7 mM) and 88 μL of potassium persulfate (140 mM) before, being placed in the dark at room temperature for 14 h) with manganese dioxide) by Brevinin-1BW at different concentrations. Error bars indicate three replicate experiments.

: ABTS diammonium salt purchased from MedChemExpress. Usage Cited in: Appl Sci. 2023 Oct 25, 13(21), 11681.; ABTS radical (a solution by reacting ABTS solution (7 mM) with potassium peroxydisulfate) scavenging activity of Iris-exosome.

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製品説明

ABTS diammonium salt (AzBTS-(NH4)2) is a substrate for horseradish peroxidase (HRP) conjugate. ABTS diammonium salt can be used to evaluate antioxidant capacity^[1]^[2].

体外実験

In vitro free radical scavenging experiment
1. Dissolve ABTS in ultrapure water (7.4 mM, 0.4 mL);
2. K2S2O8 stock solution (2.6 mM, 1.43 mL);
3. Mix the two solutions and let stand at room temperature in the dark for 12 hours;
4. Dilute 50 times with anhydrous ethanol;
5. Detect the absorbance at 734 nm.

A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color^[1]. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H₂O₂ concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H₂O₂. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

548.68

分子式

C₁₈H₂₄N₆O₆S₄

CAS 番号

30931-67-0

Appearance

Solid

Color

Light green to green

SMILES

CCN1C(C=CC(S(=O)([O-])=O)=C2)=C2S/C1=N/N=C(SC3=C4C=CC(S(=O)([O-])=O)=C3)\N4CC.[NH4+].[NH4+]

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶剤 & 溶解度

体外:

H₂O : ≥ 50 mg/mL (91.13 mM)

DMSO : 20.83 mg/mL (37.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8226 mL	9.1128 mL	18.2256 mL
5 mM	0.3645 mL	1.8226 mL	3.6451 mL
10 mM	0.1823 mL	0.9113 mL	1.8226 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 99.99%

Select Batch:

データシート (276 KB) SDS (393 KB)

COA (248 KB) HNMR (216 KB) RP-HPLC (256 KB) MS (72 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [Content Brief]

[2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164. [Content Brief]

キナーゼ実験
^[2]

Enzyme activity is determined photometrically using a temperature controlled multi-mode plate reader or alternatively in a UV/Vis spectrophotometer. Reactions are initiated by addition of the enzyme. Enzyme activity is measured over a period of 10 min at 25°C at the appropriate wavelength for the substrate. One unit (1 U) is defined as the amount of enzyme that converts 1 µmol substrate per minute. Various H₂O₂ concentrations (0-1250 µM, enzyme concentrations (0.27-54 nM) and substrate concentrations are used to determine the enzyme activity. The activity of rPsaDyP vs ABTS is determined in 100 mM sodium acetate buffer at pH 3.8 and a final H₂O₂ concentration of 125 µM. Production of the ABTS cation radical is studied at 420 nm (ε₄₂₀ 36,000 L mol^-1 cm^-1)^[2].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [Content Brief]

[2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.8226 mL	9.1128 mL	18.2256 mL	45.5639 mL
	5 mM	0.3645 mL	1.8226 mL	3.6451 mL	9.1128 mL
	10 mM	0.1823 mL	0.9113 mL	1.8226 mL	4.5564 mL
	15 mM	0.1215 mL	0.6075 mL	1.2150 mL	3.0376 mL
	20 mM	0.0911 mL	0.4556 mL	0.9113 mL	2.2782 mL
	25 mM	0.0729 mL	0.3645 mL	0.7290 mL	1.8226 mL
	30 mM	0.0608 mL	0.3038 mL	0.6075 mL	1.5188 mL
H₂O	40 mM	0.0456 mL	0.2278 mL	0.4556 mL	1.1391 mL
	50 mM	0.0365 mL	0.1823 mL	0.3645 mL	0.9113 mL
	60 mM	0.0304 mL	0.1519 mL	0.3038 mL	0.7594 mL
	80 mM	0.0228 mL	0.1139 mL	0.2278 mL	0.5695 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

ABTS diammonium salt Related Classifications

Others

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ABTS diammonium30931-67-0AzBTS-(NH4)2Fluorescent DyeInhibitorinhibitorinhibit

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ABTS (diammonium salt) (Synonyms: AzBTS-(NH4)2)

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ABTS (diammonium salt) 関連抗体

顧客検証

おすすめ

•関連小分子:

•関連タンパク質:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

ABTS (diammonium salt) 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ABTS diammonium salt Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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