AEBSF hydrochloride
Based on 18 publication(s) in Google Scholar
AEBSF hydrochloride is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin.
For research use only. We do not sell to patients.
- Purity: 99.87%
- CAS No.: 30827-99-7
- Formula: C8H11ClFNO2S
- Molecular Weight:239.69
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) AEBSF hydrochloride
More- Signal Transduct Target Ther. 2025 Dec 15;10(1):406. [Abstract]
- Autophagy. 2021 Jul;17(7):1592-1613. [Abstract]
- J Pharm Anal. 2023 Jul;13(7):788-805. [Abstract]
- Adv Healthc Mater. 2026 Apr;15(15):e04360. [Abstract]
- Int J Oncol. 2019 Jul;55(1):331-339. [Abstract]
- Int Immunopharmacol. 2025 Jun 11:161:115005. [Abstract]
- Protein Sci. 2024 Dec;33(12):e5225. [Abstract]
- Sci Rep. 2026 Mar 20;16(1):14300. [Abstract]
- J Virol. 2022 Feb 9;96(3):e0110321. [Abstract]
- Biochim Biophys Acta Mol Cell Biol Lipids. 2025 Apr 26:159618. [Abstract]
- Vet Res. 2024 Jun 14;55(1):78. [Abstract]
- Drug Metab Dispos. 2026 Mar 25.
- Arch Biochem Biophys. 2020 Jul 30;688:108402. [Abstract]
- Vet Microbiol. 2026 May:316:110968. [Abstract]
- Vet Microbiol. 2023 Apr:279:109666. [Abstract]
- Vet Microbiol. 2022 Aug:271:109494. [Abstract]
- Vet Microbiol. 2021 Feb:253:108959. [Abstract]
- bioRxiv. 2026 Mar 5:2026.03.04.709643. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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RT-PCR
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WB
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Cell Proliferation/Viability Assay
Biological Activity
AEBSF inhibits the constitutive production of Aβ by directly inhibiting β-secretase in five different human cell lines, both neural and nonneural[1].
AEBSF, as a serine protease inhibitor, inhibits the lysis of leukemic cells by human macrophages without inhibiting macrophage secretion of TNF-α and IL-1β[2].
AEBSF also disturbs the growth of blastocysts on endometrial cells and inhibit the adhesion of HeLa cells on HUVECs by altering the protein secretion pattern[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 30827-99-7
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Appearance Solid
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Molecular Weight 239.69
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Formula C8H11ClFNO2S
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Color White to off-white
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SMILES
O=S(C1=CC=C(CCN)C=C1)(F)=O.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (18)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]2025 Dec 15;10(1):406. PMID: 41392286 -
Autophagy
Discovery of a potent SCAP degrader that ameliorates HFD-induced obesity, hyperlipidemia and insulin resistance via an autophagy-independent lysosomal pathway. [Abstract]2021 Jul;17(7):1592-1613. PMID: 32432943 -
J Pharm Anal
The role of signaling crosstalk of microglia in hippocampus on progression of ageing and Alzheimer's disease. [Abstract]2023 Jul;13(7):788-805. PMID: 37577391 -
Adv Healthc Mater
An Injectable Clinical-Grade Fibrin Hydrogel Restores Fertility in Intrauterine Adhesions via MSC-Mediated Immune and Regenerative Modulation, Unlocking an Equivalent Cell-Free EV-Based Therapy. [Abstract]2026 Apr;15(15):e04360. PMID: 41660804 -
Int J Oncol
Metformin induces TPC-1 cell apoptosis through endoplasmic reticulum stress-associated pathways in vitro and in vivo. [Abstract]2019 Jul;55(1):331-339. PMID: 31180536 -
Int Immunopharmacol
Trichinella spiralis serine protease inhibitors regulate the dynamic interaction between endoplasmic reticulum stress and autophagy in host intestinal epithelial cells. [Abstract]2025 Jun 11:161:115005. PMID: 40505235
AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jun 11:161:115005. [Abstract]
AEBSF hydrochloride (50-250 μM, 2 h)The concentration of AEBSF hydrochloride and its effects on intestinal barrier. IPEC: intestinal epithelial cell
AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jun 11:161:115005. [Abstract]
The effects of AEBSF hydrochloride (100 μM) on intestinal barrier.
AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2025 Jun 11:161:115005. [Abstract]
AEBSF hydrochloride (100 μM). qPCR analyse the expression of autophagyrelated indicators after inhibiting ATF6.
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Protein Sci
Distinct substrate specificities of the three catalytic subunits of the Trichomonas vaginalis proteasome. [Abstract]2024 Dec;33(12):e5225. PMID: 39589076 -
Sci Rep
2026 Mar 20;16(1):14300. PMID: 41862552 -
J Virol
ATF6-Mediated Unfolded Protein Response Facilitates Adeno-associated Virus 2 (AAV2) Transduction by Releasing the Suppression of the AAV Receptor on Endoplasmic Reticulum Stress. [Abstract]2022 Feb 9;96(3):e0110321. PMID: 34851146 -
Biochim Biophys Acta Mol Cell Biol Lipids
2025 Apr 26:159618. PMID: 40294697 -
Vet Res
Regulatory effects of Trichinella spiralis serpin-type serine protease inhibitor on endoplasmic reticulum stress and oxidative stress in host intestinal epithelial cells. [Abstract]2024 Jun 14;55(1):78. PMID: 38877574 -
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Arch Biochem Biophys
ALA protects against ERS-mediated apoptosis in a cochlear cell model with low citrate synthase expression. [Abstract]2020 Jul 30;688:108402. PMID: 32418909
AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2020 Jul 30;688:108402. [Abstract]
Western blotting analysis of the expression level of pATF6, CHOP, Cleaved Caspase12 and Bax in shRNA-Cs1429 and shRNA-Cs1429+AEBSF cells.
AEBSF hydrochloride purchased from MedChemExpress. Usage Cited in: Arch Biochem Biophys. 2020 Jul 30;688:108402. [Abstract]
The proliferation capacity of shRNA-Cs1429 transfected cells, by treatment with AEBSF (200 μmol/l), is the highest among all the groups.
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Vet Microbiol
The PERK-eIF2α branch activates the NLRP3 inflammasome through the NF-κB signaling pathway to suppress NDV replication. [Abstract]2026 May:316:110968. PMID: 41793984 -
Vet Microbiol
GRAMD4 regulates PEDV-induced cell apoptosis inhibiting virus replication via the endoplasmic reticulum stress pathway. [Abstract]2023 Apr:279:109666. PMID: 36738512 -
Vet Microbiol
Induction and modulation of the unfolded protein response during porcine deltacoronavirus infection. [Abstract]2022 Aug:271:109494. PMID: 35752087 -
Vet Microbiol
Porcine epidemic diarrhea virus infections induce autophagy in Vero cells via ROS-dependent endoplasmic reticulum stress through PERK and IRE1 pathways. [Abstract]2021 Feb:253:108959. PMID: 33360915 -
bioRxiv
Comparative Genomic and Functional Profiling of ECM-Targeting Enzymes in Bacteroides, a Key Genus of the Human Gut Microbiome. [Abstract]2026 Mar 5:2026.03.04.709643. PMID: 41847005
Solvent & Solubility
DMSO : 100 mg/mL (417.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : ≥ 100 mg/mL (417.21 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (8.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (8.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (417.21 mM); Clear solution; Need ultrasonic
Add each solvent one by one: Saline
Solubility: 100 mg/mL (417.21 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice injected with 2.5×103 parasites are randomLy assigned to one of the treatment groups according to the treatment given: without drugs (control group), vehicle alone (vehicle control group), pyrimethamine alone at different doses, LY311727 alone at different doses, AEBSF alone at different doses or AEBSF 76.8 mg/kg plus pyrimethamine 10 mg/kg. Each treatment group consists of 10 animals. Treatment is initiated 24 h after parasite inoculation and is continued for seven consecutive days. Mouse survival is monitored daily and continued in live mice until 15 days post-infection. All experiments are performed three times and the data shown represent the cumulative results.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (417 KB)
- English - EN (417 KB)
- Français - FR (417 KB)
- Deutsch - DE (417 KB)
- Norwegian - NO (417 KB)
- Español - ES (417 KB)
- Swedish - SV (417 KB)
- Italian - IT (417 KB)
- Korean - KR (417 KB)
- Portuguese - PT (417 KB)
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Handling Instructions (2659 KB)
References
[1]. Citron M, et al. Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF. Neuron. 1996 Jul;17(1):171-9 [Content Brief]
[2]. Nakabo Y, et al. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. J Leukoc Biol. 1996 Sep;60(3):328-36. [Content Brief]
[3]. Buitrago-Rey R, et al. Evaluation of two inhibitors of invasion: LY311727 [3-(3-acetamide-1-benzyl-2-ethyl-indolyl-5-oxy)propane phosphonic acid] and AEBSF [4-(2-aminoethyl)-benzenesulphonyl fluoride] in acute murine toxoplasmosis. J Antimicrob Chemother. [Content Brief]
[4]. Jiang YH, et al. Serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) inhibits the rat embryo implantation in vivo and interferes with cell adhesion in vitro. Contraception. 2011 Dec;84(6):642-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 4.1721 mL | 20.8603 mL | 41.7206 mL | 104.3014 mL |
| 5 mM | 0.8344 mL | 4.1721 mL | 8.3441 mL | 20.8603 mL | |
| 10 mM | 0.4172 mL | 2.0860 mL | 4.1721 mL | 10.4301 mL | |
| 15 mM | 0.2781 mL | 1.3907 mL | 2.7814 mL | 6.9534 mL | |
| 20 mM | 0.2086 mL | 1.0430 mL | 2.0860 mL | 5.2151 mL | |
| 25 mM | 0.1669 mL | 0.8344 mL | 1.6688 mL | 4.1721 mL | |
| 30 mM | 0.1391 mL | 0.6953 mL | 1.3907 mL | 3.4767 mL | |
| 40 mM | 0.1043 mL | 0.5215 mL | 1.0430 mL | 2.6075 mL | |
| 50 mM | 0.0834 mL | 0.4172 mL | 0.8344 mL | 2.0860 mL | |
| 60 mM | 0.0695 mL | 0.3477 mL | 0.6953 mL | 1.7384 mL | |
| 80 mM | 0.0522 mL | 0.2608 mL | 0.5215 mL | 1.3038 mL | |
| 100 mM | 0.0417 mL | 0.2086 mL | 0.4172 mL | 1.0430 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
AEBSF hydrochloride Related Classifications
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