AEBSF hydrochloride

Cat. No.: HY-12821 Purity: 99.86%: FAQs
Data Sheet SDS COA Handling Instructions

AEBSF hydrochloride is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin.

For research use only. We do not sell to patients.

AEBSF hydrochloride Chemical Structure

CAS No. : 30827-99-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solution
10 mM * 1 mL in DMSO	USD 80	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 80	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 73	In-stock	Estimated Time of Arrival: December 31
200 mg	USD 132	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 10 publication(s) in Google Scholar

8 Publications Citing Use of MCE AEBSF hydrochloride

Cell Viability Assay

: AEBSF hydrochloride purchased from MCE. Usage Cited in: Arch Biochem Biophys. 2020 Jul 30;688:108402. [Abstract]; Western blotting analysis of the expression level of pATF6, CHOP, Cleaved Caspase12 and Bax in shRNA-Cs1429 and shRNA-Cs1429+AEBSF cells.

: AEBSF hydrochloride purchased from MCE. Usage Cited in: Arch Biochem Biophys. 2020 Jul 30;688:108402. [Abstract]; The proliferation capacity of shRNA-Cs1429 transfected cells, by treatment with AEBSF (200 μmol/l), is the highest among all the groups.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

AEBSF hydrochloride is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, plasmin, thrombin, and trypsin.

In Vitro

AEBSF inhibits the constitutive production of Aβ by directly inhibiting β-secretase in five different human cell lines, both neural and nonneural^[1].
AEBSF, as a serine protease inhibitor, inhibits the lysis of leukemic cells by human macrophages without inhibiting macrophage secretion of TNF-α and IL-1β^[2].
AEBSF also disturbs the growth of blastocysts on endometrial cells and inhibit the adhesion of HeLa cells on HUVECs by altering the protein secretion pattern^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AEBSF (76.8 mg/kg daily, i.p.) results in prolongation of the survival of mice that have been given a lethal T. gondii infection^[3].?AEBSF also reduces airway response and underlying inflammation in cockroach allergen-induced murine model^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

239.69

Appearance

Solid

Formula

C₈H₁₁ClFNO₂S

CAS No.

30827-99-7

SMILES

O=S(C1=CC=C(CCN)C=C1)(F)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (417.21 mM; Need ultrasonic)

H₂O : ≥ 100 mg/mL (417.21 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1721 mL	20.8603 mL	41.7206 mL
5 mM	0.8344 mL	4.1721 mL	8.3441 mL
10 mM	0.4172 mL	2.0860 mL	4.1721 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS
Solubility: 100 mg/mL (417.21 mM); Clear solution; Need ultrasonic
2.

Add each solvent one by one: Saline
Solubility: 100 mg/mL (417.21 mM); Clear solution; Need ultrasonic
3.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (8.68 mM); Clear solution
4.

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (8.68 mM); Clear solution
5.

Add each solvent one by one: 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (8.68 mM); Clear solution

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (247 KB) HNMR (205 KB) LCMS (113 KB)

Handling Instructions (2659 KB)

References

[1]. Citron M, et al. Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF. Neuron. 1996 Jul;17(1):171-9 [Content Brief]

[2]. Nakabo Y, et al. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. J Leukoc Biol. 1996 Sep;60(3):328-36. [Content Brief]

[3]. Buitrago-Rey R, et al. Evaluation of two inhibitors of invasion: LY311727 [3-(3-acetamide-1-benzyl-2-ethyl-indolyl-5-oxy)propane phosphonic acid] and AEBSF [4-(2-aminoethyl)-benzenesulphonyl fluoride] in acute murine toxoplasmosis. J Antimicrob Chemother. [Content Brief]

[4]. Jiang YH, et al. Serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) inhibits the rat embryo implantation in vivo and interferes with cell adhesion in vitro. Contraception. 2011 Dec;84(6):642-8. [Content Brief]

Cell Assay ^[4]	The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×10³ cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO₂ for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Mice injected with 2.5×10³ parasites are randomLy assigned to one of the treatment groups according to the treatment given: without drugs (control group), vehicle alone (vehicle control group), pyrimethamine alone at different doses, LY311727 alone at different doses, AEBSF alone at different doses or AEBSF 76.8 mg/kg plus pyrimethamine 10 mg/kg. Each treatment group consists of 10 animals. Treatment is initiated 24 h after parasite inoculation and is continued for seven consecutive days. Mouse survival is monitored daily and continued in live mice until 15 days post-infection. All experiments are performed three times and the data shown represent the cumulative results. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Citron M, et al. Inhibition of amyloid beta-protein production in neural cells by the serine protease inhibitor AEBSF. Neuron. 1996 Jul;17(1):171-9 [Content Brief] [2]. Nakabo Y, et al. Lysis of leukemic cells by human macrophages: inhibition by 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), a serine protease inhibitor. J Leukoc Biol. 1996 Sep;60(3):328-36. [Content Brief] [3]. Buitrago-Rey R, et al. Evaluation of two inhibitors of invasion: LY311727 [3-(3-acetamide-1-benzyl-2-ethyl-indolyl-5-oxy)propane phosphonic acid] and AEBSF [4-(2-aminoethyl)-benzenesulphonyl fluoride] in acute murine toxoplasmosis. J Antimicrob Chemother. [Content Brief] [4]. Jiang YH, et al. Serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) inhibits the rat embryo implantation in vivo and interferes with cell adhesion in vitro. Contraception. 2011 Dec;84(6):642-8. [Content Brief]