AdipoRon
Based on 41 publication(s) in Google Scholar
AdipoRon is an orally active adiponectin receptor (AdipoR) agonist, binding to AdipoR1 and AdipoR2 with Kds of 1.8 and 3.1 μM, respectively.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 924416-43-3
- Formula: C27H28N2O3
- Molecular Weight:428.53
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Anti-diabetic Compound Library
HY-L040
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Anti-Cardiovascular Disease Compound Library
HY-L046
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Orally Active Compound Library
HY-L061
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Anti-Obesity Compound Library
HY-L087
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Targeted Diversity Library
HY-L099
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Human Metabolite Library
HY-L123
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Osteogenesis Compound Library
HY-L131
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Metabolic Enzyme Compound Library
HY-L146
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Membrane Protein-targeted Compound Library
HY-L149
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Membrane Receptor-targeted Compound Library
HY-L150
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
Publications Citing Use of MedChemExpress (MCE) AdipoRon
More- Cancer Cell. 2025 Feb 10;43(2):269-291.e19. [Abstract]
- Redox Biol. 2022 Aug:54:102390. [Abstract]
- Mol Psychiatry. 2021 Oct;26(10):5669-5689. [Abstract]
- Stroke. 2026 Jul;57(7):2194-2207. [Abstract]
- Acta Pharmacol Sin. 2023 Feb;44(2):356-366. [Abstract]
- Free Radic Biol Med. 2025 Sep 10:S0891-5849(25)00969-4. [Abstract]
- Brain Behav Immun. 2025 May:126:160-175. [Abstract]
- Diabetes. 2021 Jun;70(6):1303-1316. [Abstract]
- Cell Rep. 2024 Dec 10;43(12):115074. [Abstract]
- EMBO Rep. 2025 Nov 17. [Abstract]
- Prog Neurobiol. 2021 Oct:205:102125. [Abstract]
- Diabetes Obes Metab. 2025 Jun 17. [Abstract]
- Cells. 2023 May 15;12(10):1393. [Abstract]
- Int J Mol Sci. 2023 Mar 18;24(6):5801. [Abstract]
- Eur J Pharmacol. 2025 Dec 10:1011:178468. [Abstract]
- Eur J Pharmacol. 2024 Nov 6:985:177111. [Abstract]
- Int Immunopharmacol. 2024 Aug 29:141:113011. [Abstract]
- Neuropharmacology. 2025 Sep 15:281:110687. [Abstract]
- J Dairy Sci. 2023 Aug;106(8):5763-5774. [Abstract]
- Mol Neurobiol. 2025 Nov 19;63(1):68. [Abstract]
- Poult Sci. 2026 Feb 1;105(4):106573. [Abstract]
- Poult Sci. 2024 Feb;103(2):103293. [Abstract]
- Poult Sci. 2023 Feb;102(2):102319. [Abstract]
- Poult Sci. 2022 Apr;101(4):101708. [Abstract]
- J Inflamm Res. 2024 Oct 24:17:7653-7674. [Abstract]
- Prog Neuropsychopharmacol Biol Psychiatry. 2023 Jul 13:125:110758. [Abstract]
- J Biol Chem. 2018 Apr 20;293(16):6064-6074. [Abstract]
- Neurochem Res. 2024 Aug;49(8):2075-2086. [Abstract]
- J Neurotrauma. 2019 Mar 19;36(6):903-918. [Abstract]
- Mol Cell Endocrinol. 2026 Jun:616:112743. [Abstract]
- Mol Med Rep. 2025 Mar;31(3):64. [Abstract]
- IUBMB Life. 2026 Jan;78(1):e70084. [Abstract]
- Diabetes Metab Syndr Obes. 2019 Oct 23;12:2165-2179. [Abstract]
- Mol Biol Rep. 2020 Sep;47(9):6561-6572. [Abstract]
- J Cell Biochem. 2020 Jun;121(5-6):3333-3344. [Abstract]
- Br Poult Sci. 2024 Sep 9:1-14. [Abstract]
- Biomed Chromatogr. 2019 Nov;33(11):e4645. [Abstract]
- bioRxiv. 2025 Sep 21.
- Patent. US20240307497A1.
- bioRxiv. 2024 September 10.
- Research Square Preprint. 2022 May.
Customer Validation & Images
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Others
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Cell Migration/Invasion Assay
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Cell Imaging/Staining
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WB
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Bio/Physico-chemical Assay
Biological Activity
Kd: 1.8 μM (AdipoR1), 3.1 μM (AdipoR2)[1]
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Cell Line
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Type | Value | Description | References |
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| RAW264.7 | IC50 |
14.99 μM
Compound: 1
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Anti-inflammatory activity in LPS induced mouse RAW264.7 cells assessed as inhibition of IL-1beta mRNA expression incubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by RT-PCR method
Anti-inflammatory activity in LPS induced mouse RAW264.7 cells assessed as inhibition of IL-1beta mRNA expression incubated for 12 hrs followed by LPS stimulation and measured after 6 hrs by RT-PCR method
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[PMID: 36215862] |
AdipoRon is an orally active and specific AdipoR agonist, binds to AdipoR1 and AdipoR2, with Kds of 1.8 and 3.1 μM. AdipoRon (50 nM-50 μM) increases AMPK phosphorylation via AdipoR1[1]. AdipoRon (50 μM) dose-dependently attenuates the expression of TNF-α and TGF-β1 in the L02 cells. AdipoRon exhibits significant and dosage-dependent growth suppression on macrophages[2]. AdipoRon treatment significantly improves cardiac functional recovery after reperfusion, and inhibits post-MI apoptosis[3]. AdipoRon exerts vasodilation by mechanisms distinct to adiponectin and induces vasorelaxation without a marked decrease in VSMC [Ca2+]i[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 924416-43-3
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Appearance Solid
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Molecular Weight 428.53
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Formula C27H28N2O3
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Color Off-white to light yellow
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SMILES
O=C(NC1CCN(CC2=CC=CC=C2)CC1)COC3=CC=C(C(C4=CC=CC=C4)=O)C=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (41)
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Journal Impact Factor
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Most Recent
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Cancer Cell
Adiponectin reduces immune checkpoint inhibitor-induced inflammation without blocking anti-tumor immunity. [Abstract]2025 Feb 10;43(2):269-291.e19. PMID: 39933899
AdipoRon purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Feb 10;43(2):269-291.e19. [Abstract]
ARG1 abundance in BMDMs upon exposure to increasing concentrations of adiponectin or adiponectin receptor agonist AdipoRon (0-12 μg/mL).
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Redox Biol
Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner. [Abstract]2022 Aug:54:102390. PMID: 35793583
AdipoRon purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug:54:102390. [Abstract]
Effects of APN knockout and AdipoRon (50 μM) treatment on neurological functional deficit scores 24 h after TBI.
AdipoRon purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug:54:102390. [Abstract]
AdipoRon (5 mg/kg). Representative images of TUNEL staining 24 hours after TBI and corresponding statistical analysis.
AdipoRon purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug:54:102390. [Abstract]
AdipoRon (5 mg/kg). Representative Western blot and statistical analysis of Bcl-2 and Bax protein levels.
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Mol Psychiatry
Chronic oral administration of adipoRon reverses cognitive impairments and ameliorates neuropathology in an Alzheimer's disease mouse model. [Abstract]2021 Oct;26(10):5669-5689. PMID: 32132650 -
Stroke
Adiponectin Fortifies White Matter After Chronic Hypoperfusion-Induced Vascular Dementia. [Abstract]2026 Jul;57(7):2194-2207. PMID: 42131908 -
Acta Pharmacol Sin
2023 Feb;44(2):356-366. PMID: 35918410
AdipoRon purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Feb;44(2):356-366. [Abstract]
Washed human platelets were incubated with AdipoRon (2, 4, 10, 20, and 40 µg/mL) or a carrier for 120 minutes, and a clot retraction test was performed.
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Free Radic Biol Med
Targeting ER stress/ROS-driven VEGF signaling axis: AdipoRon as a multifunctional therapeutic agent for alkali burn-induced corneal neovascularization. [Abstract]2025 Sep 10:S0891-5849(25)00969-4. PMID: 40939851 -
Brain Behav Immun
Adiponectin ameliorates traumatic brain injury-induced ferroptosis through AMPK- ACC1 signaling pathway. [Abstract]2025 May:126:160-175. PMID: 39947491 -
Diabetes
Adiponectin Alleviates Diet-Induced Inflammation in the Liver by Suppressing MCP-1 Expression and Macrophage Infiltration. [Abstract]2021 Jun;70(6):1303-1316. PMID: 34162682 -
Cell Rep
Adiponectin receptor 1-mediated basolateral amygdala-prelimbic cortex circuit regulates methamphetamine-associated memory. [Abstract]2024 Dec 10;43(12):115074. PMID: 39661515 -
EMBO Rep
2025 Nov 17. PMID: 41249580 -
Prog Neurobiol
Adiponectin ameliorates hypoperfusive cognitive deficits by boosting a neuroprotective microglial response. [Abstract]2021 Oct:205:102125. PMID: 34333040 -
Diabetes Obes Metab
AdipoRon attenuates steatosis, inflammation and fibrosis in murine diet-induced NASH via inhibiting ER stress. [Abstract]2025 Jun 17. PMID: 40528684 -
Cells
APN Expression in Serum and Corpus Luteum: Regulation of Luteal Steroidogenesis Is Possibly Dependent on the AdipoR2/AMPK Pathway in Goats. [Abstract]2023 May 15;12(10):1393. PMID: 37408227 -
Int J Mol Sci
Adiponectin Enhances Fatty Acid Signaling in Human Taste Cells by Increasing Surface Expression of CD36. [Abstract]2023 Mar 18;24(6):5801. PMID: 36982874 -
Eur J Pharmacol
AdipoRon ameliorates anxiety- and depression-like behaviors in chronic restraint-stressed mice via AMPK-PPARα-BDNF-TrkB signaling. [Abstract]2025 Dec 10:1011:178468. PMID: 41386463 -
Eur J Pharmacol
AdipoRon improves mitochondrial homeostasis and protects dopaminergic neurons through activation of the AMPK signaling pathway in the 6-OHDA-lesioned rats. [Abstract]2024 Nov 6:985:177111. PMID: 39515564 -
Int Immunopharmacol
AdipoRon exerts an antidepressant effect by inhibiting NLRP3 inflammasome activation in microglia via promoting mitophagy. [Abstract]2024 Aug 29:141:113011. PMID: 39213872 -
Neuropharmacology
Intranasal AdipoRon improves motor function in a rat model of Parkinson's disease by promoting neurogenesis in the nigrostriatal pathway. [Abstract]2025 Sep 15:281:110687. PMID: 40962109 -
J Dairy Sci
AdipoRon alleviates fatty acid-induced lipid accumulation and mitochondrial dysfunction in bovine hepatocytes by promoting autophagy. [Abstract]2023 Aug;106(8):5763-5774. PMID: 37268562 -
Mol Neurobiol
Adiponectin Signaling Ameliorates Cognitive Dysfunction in Type 2 Diabetic Mice by Activating the Hippocampal AdipoR2-PPARα/CREB Pathway. [Abstract]2025 Nov 19;63(1):68. PMID: 41254427 -
Poult Sci
2026 Feb 1;105(4):106573. PMID: 41650643 -
Poult Sci
Osteocalcin activates lipophagy via the ADPN-AMPK/PPARα-mTOR signaling pathway in chicken embryonic hepatocyte. [Abstract]2024 Feb;103(2):103293. PMID: 38070403 -
Poult Sci
The adiponectin receptor agonist, AdipoRon, promotes reproductive hormone secretion and gonadal development via the hypothalamic-pituitary-gonadal axis in chickens. [Abstract]2023 Feb;102(2):102319. PMID: 36512870 -
Poult Sci
2022 Apr;101(4):101708. PMID: 35150940 -
J Inflamm Res
Unleashing AdipoRon's Potential: A Fresh Approach to Tackle Pseudomonas aeruginosa Infections in Bronchiectasis via Sphingosine Metabolism Modulation. [Abstract]2024 Oct 24:17:7653-7674. PMID: 39469062 -
Prog Neuropsychopharmacol Biol Psychiatry
PPARγ/Adiponectin axis attenuates methamphetamine-induced conditional place preference via the hippocampal AdipoR1 signaling pathway. [Abstract]2023 Jul 13:125:110758. PMID: 36972780 -
J Biol Chem
Alternative splicing variant of the scaffold protein APPL1 suppresses hepatic adiponectin signaling and function. [Abstract]2018 Apr 20;293(16):6064-6074. PMID: 29483192
AdipoRon purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2018 Apr 20;293(16):6064-6074. [Abstract]
Interaction of APPL1sv with adiponectin receptors in cells. Mouse hepatocytes are serum-starved for 4 h and treated with or without 50 μM AdipoRon (Ad) for 0, 5, or 10 min.
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Neurochem Res
AdipoRon Ameliorates Synaptic Dysfunction and Inhibits tau Hyperphosphorylation through the AdipoR/AMPK/mTOR Pathway in T2DM Mice. [Abstract]2024 Aug;49(8):2075-2086. PMID: 38819697 -
J Neurotrauma
Activating Adiponectin Signaling with Exogenous AdipoRon Reduces Myelin Lipid Accumulation and Suppresses Macrophage Recruitment after Spinal Cord Injury. [Abstract]2019 Mar 19;36(6):903-918. PMID: 30221582
AdipoRon purchased from MedChemExpress. Usage Cited in: J Neurotrauma. 2019 Mar 19;36(6):903-918. [Abstract]
WT macrophages are incubated with myelin debris for 3 hours to obtain mye-MΦ, and further treated with AdipoRon for another 48 hours. ABCA1 and ABCG1 levels are assessed by western blot.
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Mol Cell Endocrinol
Adiponectin upregulates irisin expression through the APPL1/p38MAPK/PGC-1α signalling pathway in murine skeletal muscle. [Abstract]2026 Jun:616:112743. PMID: 41654165 -
Mol Med Rep
Adiponectin targets the AMPK/mTOR signaling pathway to alleviate cognitive impairment in epilepsy. [Abstract]2025 Mar;31(3):64. PMID: 39749696 -
IUBMB Life
AdipoRon Suppresses Multiple Myeloma Proliferation Through AMPK-Mediated Metabolic Reprogramming and Apoptosis Induction. [Abstract]2026 Jan;78(1):e70084. PMID: 41503835 -
Diabetes Metab Syndr Obes
AdipoRon Alleviates Free Fatty Acid-Induced Myocardial Cell Injury Via Suppressing Nlrp3 Inflammasome Activation. [Abstract]2019 Oct 23;12:2165-2179. PMID: 31749627 -
Mol Biol Rep
Anti-inflammatory and anti-proliferative action of adiponectin mediated by insulin signaling cascade in human vascular smooth muscle cells. [Abstract]2020 Sep;47(9):6561-6572. PMID: 32789574 -
J Cell Biochem
Adiponectin receptor agonist AdipoRon attenuates calcification of osteoarthritis chondrocytes by promoting autophagy. [Abstract]2020 Jun;121(5-6):3333-3344. PMID: 31898335 -
Br Poult Sci
Effect of goose-derived adiponectin peptide gADP3 on LPS-induced inflammatory injury in goose liver. [Abstract]2024 Sep 9:1-14. PMID: 39249992 -
Biomed Chromatogr
Characterization of the metabolite of AdipoRon in rat and human liver microsomes by ultra-high-performance liquid chromatography combined with Q-Exactive Orbitrap tandem mass spectrometry. [Abstract]2019 Nov;33(11):e4645. PMID: 31306503 -
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Solvent & Solubility
DMSO : 62.5 mg/mL (145.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
The effects of AdipoRon on the proliferation of parenchymal and non-parenchymal hepatocytes are evaluated in vitro via L02 and RAW264.7, by MTT assay as described with slight modification: 100 μL cells suspension (6×104/mL) are seeded in a 96-well plate and incubated for 18 h. Fresh media with AdipoRon are added at specified concentrations, and the incubations continue for a further 24 h. Then cells are incubated for 4 h with 0.5 mg/mL of MTT, and analyzed in a microplate reader at 490 nm. Each group is performed in six replications. The mean absorbance values corrected for a blank (medium only) are calculated as percentages of survival[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[2]
After 3 days of acclimation, mice are randomLy divided into six groups (9 mice in each): control, model, bicyclol (20 mg/kg), AdipoRon (0.02 mg/kg, 0.1 mg/kg, 0.5 mg/kg). The synthetic AdipoRon and bicyclol are dissolved in DMSO and diluted by saline containing 0.5% sodium carboxymethyl cellulose (CMC-Na) [final vehicle: 5% DMSO (v/v) saline solution]. All test groups are administered with vehicle (control and model groups) or therapeutic agents (bicyclol or AdipoRon groups) at a dosing volume of 10 mL/kg, by intragastric (i.g.) gavage twice per day for three consecutive days prior to D-GalN administration. 2 h after last treatment, mice are challenged with a single intraperitoneal (i.p.) administration of D-GalN saline solution at a dose of 600 mg/kg to induce acute liver injury, while the control group mice receive saline instead. Then mice are fasted for 20 h before orbital blood collection. Finally, all animals are sacrificed by cervical dislocation, and livers are harvested for biochemical or histopathology analysis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Okada-Iwabu M, et al. A small-molecule AdipoR agonist for type 2 diabetes and short life in obesity. Nature. 2013 Nov 28;503(7477):493-9. [Content Brief]
[2]. Wang Y, et al. Hepatoprotective effects of AdipoRon against d-galactosamine-induced liver injury in mice. Eur J Pharm Sci. 2016 Aug 9;93:123-131. [Content Brief]
[3]. Zhang Y, et al. AdipoRon, the first orally active adiponectin receptor activator, attenuates postischemic myocardial apoptosis through both AMPK-mediated and AMPK-independent signalings. Am J Physiol Endocrinol Metab. 2015 Aug 1;309(3):E275-82. [Content Brief]
[4]. Hong K, et al. Adiponectin Receptor Agonist, AdipoRon, Causes Vasorelaxation Predominantly Via a Direct Smooth Muscle Action. Microcirculation. 2016 Apr;23(3):207-20. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3335 mL | 11.6677 mL | 23.3355 mL | 58.3387 mL |
| 5 mM | 0.4667 mL | 2.3335 mL | 4.6671 mL | 11.6677 mL | |
| 10 mM | 0.2334 mL | 1.1668 mL | 2.3335 mL | 5.8339 mL | |
| 15 mM | 0.1556 mL | 0.7778 mL | 1.5557 mL | 3.8892 mL | |
| 20 mM | 0.1167 mL | 0.5834 mL | 1.1668 mL | 2.9169 mL | |
| 25 mM | 0.0933 mL | 0.4667 mL | 0.9334 mL | 2.3335 mL | |
| 30 mM | 0.0778 mL | 0.3889 mL | 0.7778 mL | 1.9446 mL | |
| 40 mM | 0.0583 mL | 0.2917 mL | 0.5834 mL | 1.4585 mL | |
| 50 mM | 0.0467 mL | 0.2334 mL | 0.4667 mL | 1.1668 mL | |
| 60 mM | 0.0389 mL | 0.1945 mL | 0.3889 mL | 0.9723 mL | |
| 80 mM | 0.0292 mL | 0.1458 mL | 0.2917 mL | 0.7292 mL | |
| 100 mM | 0.0233 mL | 0.1167 mL | 0.2334 mL | 0.5834 mL |
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