2. Metabolic Enzyme/Protease
  3. Adiponectin Receptor
  4. AdipoRon hydrochloride

AdipoRon hydrochloride is an orally active and specific AdipoR agonist, binding to AdipoR1 and AdipoR2, with Kds of 1.8 and 3.1 μM, respectively.

For research use only. We do not sell to patients.

CAS No. : 1781835-20-8

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Customer Review

Based on 40 publication(s) in Google Scholar

Other Forms of AdipoRon hydrochloride:

AdipoRon hydrochloride Related Antibodies

Top Publications Citing Use of Products

40 Publications Citing Use of MCE AdipoRon hydrochloride

Others
Cell Migration/Invasion Assay
Bio/Physico-chemical Assay
Cell Imaging/Staining
WB

    AdipoRon hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Feb 10;43(2):269-291.e19.  [Abstract]

    ARG1 abundance in BMDMs upon exposure to increasing concentrations of adiponectin or adiponectin receptor agonist AdipoRon (0-12 μg/mL).

    AdipoRon hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug:54:102390.

    Effects of APN knockout and AdipoRon (50 μM) treatment on neurological functional deficit scores 24 h after TBI.

    AdipoRon hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug:54:102390.

    AdipoRon (5 mg/kg). Representative images of TUNEL staining 24 hours after TBI and corresponding statistical analysis.

    AdipoRon hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Aug:54:102390.

    AdipoRon (5 mg/kg). Representative Western blot and statistical analysis of Bcl-2 and Bax protein levels.

    AdipoRon hydrochloride purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Feb;44(2):356-366.  [Abstract]

    Washed human platelets were incubated with AdipoRon (2, 4, 10, 20, and 40 µg/mL) or a carrier for 120 minutes, and a clot retraction test was performed.

    AdipoRon hydrochloride purchased from MedChemExpress. Usage Cited in: J Neurotrauma. 2019 Mar 19;36(6):903-918.  [Abstract]

    WT macrophages are incubated with myelin debris for 3 hours to obtain mye-MΦ, and further treated with AdipoRon for another 48 hours. ABCA1 and ABCG1 levels are assessed by western blot.

    AdipoRon hydrochloride purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2018 Apr 20;293(16):6064-6074.  [Abstract]

    Interaction of APPL1sv with adiponectin receptors in cells. Mouse hepatocytes are serum-starved for 4 h and treated with or without 50 μM AdipoRon (Ad) for 0, 5, or 10 min.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    AdipoRon hydrochloride is an orally active and specific AdipoR agonist, binding to AdipoR1 and AdipoR2, with Kds of 1.8 and 3.1 μM, respectively.

    IC50 & Target

    Kd: 1.8 μM (AdipoR1), 3.1 μM (AdipoR2)[1]
    In Vitro

    AdipoRon hydrochloride is an orally active and specific AdipoR agonist, binds to AdipoR1 and AdipoR2, with Kds of 1.8 and 3.1 μM. AdipoRon (50 nM-50 μM) increases AMPK phosphorylation via AdipoR1[1]. AdipoRon (50 μM) dose-dependently attenuates the expression of TNF-α and TGF-β1 in the L02 cells. AdipoRon exhibits significant and dosage-dependent growth suppression on macrophages[2]. AdipoRon treatment significantly improves cardiac functional recovery after reperfusion, and inhibits post-MI apoptosis[3]. AdipoRon exerts vasodilation by mechanisms distinct to adiponectin and induces vasorelaxation without a marked decrease in VSMC [Ca2+]i[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AdipoRon hydrochloride Related Antibodies
    In Vivo

    AdipoRon (50 mg/kg, i.v.) cuases significant phosphorylation of AMPK in skeletal muscle and liver of wild-type mice but not Adipor1 / Adipor2 / double-knockout mice[1]. AdipoRon (0.02, 0.1, and 0.5 mg/kg, i.g.) alleviates D-GalN induced hepatotoxicity in mice, and prevents hepatic architecture distortion against D-GalN challenge. The hepatoprotective potential of AdipoRon is particularly evident in higher dosages (0.1 and 0.5 mg/kg)[2]. Enhanced cardiomyocyte apoptosis in APN-deficient mice is rescued by AdipoRon (50 mg/kg, p.o.) administration. Antiapoptotic effect of AdipoRon is attenuated but not lost in AMPK-DN mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    464.98

    Formula

    C27H29ClN2O3
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(C1=CC=CC=C1)C2=CC=C(OCC(NC3CCN(CC4=CC=CC=C4)CC3)=O)C=C2.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (107.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1506 mL 10.7532 mL 21.5063 mL
    5 mM 0.4301 mL 2.1506 mL 4.3013 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (10.75 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 5 mg/mL (10.75 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References
    Cell Assay
    [2]

    The effects of AdipoRon on the proliferation of parenchymal and non-parenchymal hepatocytes are evaluated in vitro via L02 and RAW264.7, by MTT assay as described with slight modification: 100 μL cells suspension (6×104/mL) are seeded in a 96-well plate and incubated for 18 h. Fresh media with AdipoRon are added at specified concentrations, and the incubations continue for a further 24 h. Then cells are incubated for 4 h with 0.5 mg/mL of MTT, and analyzed in a microplate reader at 490 nm. Each group is performed in six replications. The mean absorbance values corrected for a blank (medium only) are calculated as percentages of survival[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    After 3 days of acclimation, mice are randomLy divided into six groups (9 mice in each): control, model, bicyclol (20 mg/kg), AdipoRon (0.02 mg/kg, 0.1 mg/kg, 0.5 mg/kg). The synthetic AdipoRon and bicyclol are dissolved in DMSO and diluted by saline containing 0.5% sodium carboxymethyl cellulose (CMC-Na) [final vehicle: 5% DMSO (v/v) saline solution]. All test groups are administered with vehicle (control and model groups) or therapeutic agents (bicyclol or AdipoRon groups) at a dosing volume of 10 mL/kg, by intragastric (i.g.) gavage twice per day for three consecutive days prior to D-GalN administration. 2 h after last treatment, mice are challenged with a single intraperitoneal (i.p.) administration of D-GalN saline solution at a dose of 600 mg/kg to induce acute liver injury, while the control group mice receive saline instead. Then mice are fasted for 20 h before orbital blood collection. Finally, all animals are sacrificed by cervical dislocation, and livers are harvested for biochemical or histopathology analysis[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1506 mL 10.7532 mL 21.5063 mL 53.7658 mL
    5 mM 0.4301 mL 2.1506 mL 4.3013 mL 10.7532 mL
    10 mM 0.2151 mL 1.0753 mL 2.1506 mL 5.3766 mL
    15 mM 0.1434 mL 0.7169 mL 1.4338 mL 3.5844 mL
    20 mM 0.1075 mL 0.5377 mL 1.0753 mL 2.6883 mL
    25 mM 0.0860 mL 0.4301 mL 0.8603 mL 2.1506 mL
    30 mM 0.0717 mL 0.3584 mL 0.7169 mL 1.7922 mL
    40 mM 0.0538 mL 0.2688 mL 0.5377 mL 1.3441 mL
    50 mM 0.0430 mL 0.2151 mL 0.4301 mL 1.0753 mL
    60 mM 0.0358 mL 0.1792 mL 0.3584 mL 0.8961 mL
    80 mM 0.0269 mL 0.1344 mL 0.2688 mL 0.6721 mL
    100 mM 0.0215 mL 0.1075 mL 0.2151 mL 0.5377 mL
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    AdipoRon hydrochloride Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AdipoRon1781835-20-8Adiponectin ReceptorAdipoRsInhibitorinhibitorinhibit

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