BAY 73-6691
Based on 2 publication(s) in Google Scholar
BAY 73-6691 ((R)-BAY 73-6691) is a potent, brain penetrant, and selective PDE9A inhibitor.
For research use only. We do not sell to patients.
- Purity: 99.38%
- CAS No.: 794568-92-6
- Formula: C15H12ClF3N4O
- Molecular Weight:356.73
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) BAY 73-6691
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Biological Activity
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PDE9 |
The BAY 73-6691 dose-dependently alleviates cell viability loss due to Aβ25-35 treatment. It is found that when SH-SY5Y cells are cultured by Aβ25-35, a high degree of cell apoptosis is observed, while additional stimulation with BAY 73-6691 causes attenuation of cell apoptosis. BAY 73-6691 dose-dependently attenuates oxidative stress induced by Aβ25-35, and BAY 73-6691 at 200 μg/mL almost neutralizes Aβ25-35-induced oxidative damage. The BAY 73-6691 attenuates Aβ25-35-induced increase of apoptosis cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 794568-92-6
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Appearance Solid
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Molecular Weight 356.73
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Formula C15H12ClF3N4O
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Color Light yellow to yellow
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SMILES
O=C1C2=C(N(C3=CC=CC=C3Cl)N=C2)NC(C[C@@H](C)C(F)(F)F)=N1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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EMBO J
E3 ubiquitin ligase CHIP facilitates cAMP and cGMP signalling cross-talk by polyubiquitinating PDE9A. [Abstract]2025 Feb;44(4):1249-1273. PMID: 39806097 -
Solvent & Solubility
DMSO : ≥ 100 mg/mL (280.32 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.01 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
The SH-SY5Y human neuroblastoma cell line is used in this study. The cells are routinely cultured in a mixture of Dulbecco's modified Eagle's medium (DMEM)/Ham's F12 containing 10% fetal bovine serum, 2 mM L-glutamine, antibiotic and antimycotic solution under a humidified atmosphere of 5% CO2-95% air at 37°C. The SH-SY5Y are plated in 96-well plates at 1×105 cells per well for the treatment with Aβ25-35 and the BAY 73-6691. Before experiments, freshly prepared Aβ25-35 peptide at 20 μM is added to the cells with or without exposure to different concentrations (50, 100, 150 and 200 μg/mL) of BAY 73-6691[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Male ICR mice (weighing 25 to 30 g) are used to induce the animal model of Alzheimer's disease (AD). All mice are housed in a temperature- and humidity-controlled room with a constant light-dark cycle (12 h/12 h) and are maintained on ad libitum food and water. BAY 73-6691 at different doses (0.3, 1 and 3 mg/kg) is consecutively injected (i.p.) once daily at 7:30 A.M on days 1 to 10 after injection of Aβ25-35 (day 0). Mice are divided into six groups: (I) sham, (II) Aβ, (III) Aβ+0.3 mg/kg BAY 73-6691, (IV) Aβ+1 mg/kg BAY 73-6691, (V) Aβ+3 mg/kg BAY 73-6691 and (VI) 3 mg/kg BAY 73-6691[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8032 mL | 14.0162 mL | 28.0324 mL | 70.0810 mL |
| 5 mM | 0.5606 mL | 2.8032 mL | 5.6065 mL | 14.0162 mL | |
| 10 mM | 0.2803 mL | 1.4016 mL | 2.8032 mL | 7.0081 mL | |
| 15 mM | 0.1869 mL | 0.9344 mL | 1.8688 mL | 4.6721 mL | |
| 20 mM | 0.1402 mL | 0.7008 mL | 1.4016 mL | 3.5041 mL | |
| 25 mM | 0.1121 mL | 0.5606 mL | 1.1213 mL | 2.8032 mL | |
| 30 mM | 0.0934 mL | 0.4672 mL | 0.9344 mL | 2.3360 mL | |
| 40 mM | 0.0701 mL | 0.3504 mL | 0.7008 mL | 1.7520 mL | |
| 50 mM | 0.0561 mL | 0.2803 mL | 0.5606 mL | 1.4016 mL | |
| 60 mM | 0.0467 mL | 0.2336 mL | 0.4672 mL | 1.1680 mL | |
| 80 mM | 0.0350 mL | 0.1752 mL | 0.3504 mL | 0.8760 mL | |
| 100 mM | 0.0280 mL | 0.1402 mL | 0.2803 mL | 0.7008 mL |
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