Calmagite 

Calmagite is a chromomeric reagent which can be used to detect calcium and magnesium in various samples^[1][2][3].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of Calmagite Staining Solution^[3]
40 mg Calmagite dissolved in 100 ml water
2. Analysis of biological materials
Serum: 50 μL serum was added to each tube directly, mixed with 2 mL buffer and 0.5 mL EGTA solution, and made up to 4 mL with water. Finally 1 mL Calmagite solution was added for color development, In addition, two standards containing 1 and 1.5 μg Mg²⁺ were always run parallel to check the dependability of the earlier standard curve.
3. Evaluate and compare the magnesium levels in serum and saliva of individuals with tobacco habits with and without potentially malignant disorders^[2]
3.1 Serum collection
Two millilitres of blood was collected in plain red vacutainers by venipuncture using aseptic measures. At 3500 rpm, the serum was separated by centrifugation for 10 min.
3.2 Unstimulated saliva collection
Three millilitres of unstimulated whole saliva sample was collected from the mouth directly into the Eppendorf on a single occasion to press their tongue against their palate so that freshly secreted saliva was collected in the sublingual region for 5 min. Saliva was centrifuged at 3500 rpm for 10 min to remove the excess mucus and unwanted particles.
3.3 Serum and saliva estimation
The estimation of magnesium in serum and saliva was done by calmagite method.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

358.37

C₁₇H₁₄N₂O₅S

3147-14-6

Solid

Light brown to black

O=S(C1=C2C=CC=CC2=C(/N=N/C3=CC(C)=CC=C3O)C(O)=C1)(O)=O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Rasouli Z, et al. Simultaneously detection of calcium and magnesium in various samples by calmagite and chemometrics data processing. Spectrochim Acta A Mol Biomol Spectrosc. 2016 Dec 5;169:72-81.  [Content Brief]

  [2]. Singhal I, et al. Evaluation of magnesium levels in serum and saliva by calmagite method in individuals with tobacco habits with or without potentially malignant disorders. J Oral Maxillofac Pathol. 2023 Apr-Jun;27(2):425.  [Content Brief]

  [3]. Chauhan UP, et al. Use of calmagite for the determination of traces of magnesium in biological materials. Anal Biochem. 1969 Oct 15;32(1):70-80.  [Content Brief]