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  4. DTNB

DTNB  (Synonyms: Ellman’s Reagent; 2-ニトロ安息香酸; 2-nitrobenzoic acid; 5,5'-ジチオビス)

製品番号: HY-15915 純度: 99.80%
COA 取扱説明書 Technical Support

DTNB (Ellman’s Reagent) is a chemical used to quantify the number or concentration of thiol groups.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 69-78-3

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in DMSO USD 33 在庫あり
Solid
1 g $30 在庫あり
5 g $65 在庫あり
10 g $115 在庫あり
25 g $230 在庫あり
50 g $368 在庫あり
100 g $590 在庫あり
> 100 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 14 publication(s) in Google Scholar

DTNB 関連抗体

Top Publications Citing Use of Products

    DTNB purchased from MedChemExpress. Usage Cited in: Chem Eng J. 2025 Nov 24;526:171230.

    The results of the DTNB assay (1 mM, 30 min) are presented. These results suggested that the GMZM microneedle depleted glutathione (GSH) via NIR assistance.

    DTNB purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2024 Oct 2;16(39):52068-52079.  [Abstract]

    GSH consumption by Ru4 (10 μM) over time, with DTNB (10 mg/mL, 5 μL; 30 mim) as the GSH indicator (n = 3, mean ± SD).

    DTNB purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Jul 21;287:121688.  [Abstract]

    DTNB (20 mM; 10 min) probe was utilized to detect MnOx NSs (100 μg/mL)-mediated GSH depletion. Notably, apart from ROS generation, MnOx NSs feature GSH depletion properties, because the characteristic absorption peak of DTNB at 412 nm decreases sharply over time in the presence of MnOx NSs.

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    製品説明

    DTNB (Ellman’s Reagent) is a chemical used to quantify the number or concentration of thiol groups[1].

    体外実験

    DTNB reacts with the free sulfhydryl side chain of cysteine to form an S-S bond between the protein and a thionitrobenzoic acid (TNB) residue[1].
    The main advantage of DTNB over alternative reagents (e.g., N-ethylmaleimide or iodoacetamide) is in the selectivity of this reagent and in the ability to follow the course of the reaction spectrophotometrically[1].
    1. Preparation of solution
    1.1 Reaction Buffer: 0.1M sodium phosphate, pH 8.0, containing 1mM EDTA
    2.1 DTNB Solution: Dissolve 4 mg DTNB in 1 mL of Reaction Buffer.

    2. Detection
    2.1 Add 250 µL of sample to a test tube containing 50 µL of DTNB solution.
    Note: For unknown samples, dilution can be performed to ensure that the thiol concentration in the sample is less than 1.0 mM.
    2.2 Mix and incubate at room temperature for 15 minutes.
    2.3 With a spectrophotometer set to 412 nm and then measure absorbance of each sample.
    2.4 Calculate the amount and concentration of sulfhydryls in the sample from the molar extinction coefficient of TNB (14.15 mM-1 cm-1).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DTNB 関連抗体
    分子量

    396.35

    分子式

    C14H8N2O8S2
    CAS 番号
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C(O)C1=C([N+]([O-])=O)C=CC(SSC2=CC(C(O)=O)=C([N+]([O-])=O)C=C2)=C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (252.30 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5230 mL 12.6151 mL 25.2302 mL
    5 mM 0.5046 mL 2.5230 mL 5.0460 mL
    10 mM 0.2523 mL 1.2615 mL 2.5230 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.25 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (5.25 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.80%

    COA (249 KB) HNMR (117 KB) RP-HPLC (252 KB) LCMS (233 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5230 mL 12.6151 mL 25.2302 mL 63.0756 mL
    5 mM 0.5046 mL 2.5230 mL 5.0460 mL 12.6151 mL
    10 mM 0.2523 mL 1.2615 mL 2.5230 mL 6.3076 mL
    15 mM 0.1682 mL 0.8410 mL 1.6820 mL 4.2050 mL
    20 mM 0.1262 mL 0.6308 mL 1.2615 mL 3.1538 mL
    25 mM 0.1009 mL 0.5046 mL 1.0092 mL 2.5230 mL
    30 mM 0.0841 mL 0.4205 mL 0.8410 mL 2.1025 mL
    40 mM 0.0631 mL 0.3154 mL 0.6308 mL 1.5769 mL
    50 mM 0.0505 mL 0.2523 mL 0.5046 mL 1.2615 mL
    60 mM 0.0421 mL 0.2103 mL 0.4205 mL 1.0513 mL
    80 mM 0.0315 mL 0.1577 mL 0.3154 mL 0.7884 mL
    100 mM 0.0252 mL 0.1262 mL 0.2523 mL 0.6308 mL
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    DTNB Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DTNB69-78-3Ellman’s ReagentBiochemical Assay ReagentsInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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