Fluo-4 AM

Name: Fluo-4 AM
Brand: MedChemExpress
SKU: HY-101896
Rating: 5.0 (56 reviews)

Art. -Nr.: HY-101896 Reinheit: 97.69%: Data Sheet
COA

SDS
Handling Instructions
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Fluo-4 AM is a cell-permeable Ca²⁺ (calcium ion) indicator (Ex/Em = 485/526 nm).

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr. : 273221-67-3

Größe	Verfügbarkeit	Menge
100 μg	Auf Lager	Estimated Time of Arrival: December 31
500 μg	Auf Lager	Estimated Time of Arrival: December 31
1 mg	Auf Lager	Estimated Time of Arrival: December 31
5 mg	Auf Lager	Estimated Time of Arrival: December 31
10 mg	Auf Lager	Estimated Time of Arrival: December 31
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100 mg	Angebot einholen

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Based on 56 publication(s) in Google Scholar

Other Forms of Fluo-4 AM:

Fluo-4 AM (solution) In-stock

56 Publications Citing Use of MCE Fluo-4 AM

: Fluo-4 AM purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2022 May 17;13:855626. [Abstract]; Intracellular Ca²⁺ is measured by Fluo-4/AM (for 30 min) with flow cytometry.

: Fluo-4 AM purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Feb 10;2021:8884922.; NP cells are incubated with 5 μM Fura-4-AM for 30 min at 37°C in the dark. Intracellular Ca²⁺ levels measured using the specific Ca²⁺-sensitive fluorescent indicator Fura-4-AM and analyzed via fluorescence microscopy.

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•Related Proteins:

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Beschreibung

Fluo-4 AM is a cell-permeable Ca²⁺ (calcium ion) indicator (Ex/Em = 485/526 nm)^[1].

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)
. 1. Solution preparation^[1]
1.1 Preparation of storage solution
Solvent: DMSO
Concentration: 1 mM is recommended (e.g. weigh 100 μg Fluo-4 AM and dissolve in 96.16 μL solvent).
Storage: Use as soon as possible and avoid long-term storage.
1.2 Preparation of working solution
Dilute with buffer (e.g. HHBS containing 0.04% Poloxamer 407 (F127) (HY-D1005)) to 1 M (optimized according to the experiment).
Note: The working solution should be prepared and used immediately, and protected from light.

2. Cell staining

2.1 Count the cells and take 10⁶ cells from each sample (control and experiment/s).
2.2 Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.
2.3 Resuspend the cells in PBS and add Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 °C for 1 h.
2. 4 Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1 mL PBS.
2.5 Evaluate the staining by flow cytometry (Ex/Em = 485/526 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

1096.94

Formel

C₅₁H₅₀F₂N₂O₂₃

CAS. Nr.

273221-67-3

Appearance

Solid

Color

Brown to red

Emission (Em)

526

Excitation (Ex)

485

SMILES

O=C(OCOC(C)=O)CN(C1=CC=C(C2=C3C=C(F)C(C=C3OC4=C2C=C(F)C(OCOC(C)=O)=C4)=O)C=C1OCCOC5=CC(C)=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 10 mg/mL (9.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9116 mL	4.5581 mL	9.1163 mL
5 mM	0.1823 mL	0.9116 mL	1.8233 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molaritätsrechner
Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Reinheit & Dokumentation

Purity: ≥99.0%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (255 KB)

Handling Instructions (2659 KB)

Dyeing Example

Description: Fluo-4 AM can be used to determine total intracellular Ca²⁺ levels.
Method: For cell labeling.
1. Count cells and take 10⁶ cells from each sample.
2. Collect cells (5 min, 3000×g, 4°C) and wash once in PBS.
3. Resuspend cells in 0.5 mL PBS and add Fluo-4 AM (1 mM; 0.5 μL; 37°C; 1 h) to a final concentration of 1 μM.
4. Wash cells three times (2 min, 3000×g) with PBS and finally resuspend in 1 mL PBS, and separated into 2 flow cytometry tubes-0.5 mL in each.
5. Evaluate staining by flow cytometry and analyze the data by a software such as CellQuest software.

Verweise

[1]. Gee KR, et al. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106. [Content Brief]

[2]. Fluo-4.

Zellassay
^[1]

For measuring fluorescence from cells in suspension, dilutions to 2 to 3×10⁶ cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 μM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer^[1].

MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.

Verweise

[1]. Gee KR, et al. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106. [Content Brief]

[2]. Fluo-4.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9116 mL	4.5581 mL	9.1163 mL	22.7907 mL
DMSO	5 mM	0.1823 mL	0.9116 mL	1.8233 mL	4.5581 mL

Fluo-4 AM Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluo-4 AM273221-67-3Fluorescent DyeInhibitorinhibitorinhibit

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Fluo-4 AM

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Other Forms of Fluo-4 AM:

Fluo-4 AM Related Antibodies

56 Publications Citing Use of MCE Fluo-4 AM

Spezialitätsempfehlungen

•Related Small Molecules:

•Related Proteins:

Biologische Aktivität

Protokoll

Reinheit & Dokumentation

Verweise

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Fluo-4 AM Related Antibodies

Molaritätsrechner

Verdünnungsrechner

Complete Stock Solution Preparation Table

Fluo-4 AM Related Classifications

Keywords:

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