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  3. Fluorescent Dye
  4. Fluo-4 AM

Fluo-4 AM is a cell-permeable Ca2+ (calcium ion) indicator (Ex/Em = 485/526 nm).

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No. CAS : 273221-67-3

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Revisión del cliente

Based on 56 publication(s) in Google Scholar

Other Forms of Fluo-4 AM:

Fluo-4 AM Related Antibodies

Top Publications Citing Use of Products

56 Publications Citing Use of MCE Fluo-4 AM

IF

    Fluo-4 AM purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2022 May 17;13:855626.  [Abstract]

    Intracellular Ca2+ is measured by Fluo-4/AM (for 30 min) with flow cytometry.

    Fluo-4 AM purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Feb 10;2021:8884922.

    NP cells are incubated with 5 μM Fura-4-AM for 30 min at 37°C in the dark. Intracellular Ca2+ levels measured using the specific Ca2+-sensitive fluorescent indicator Fura-4-AM and analyzed via fluorescence microscopy.

    • Actividad biológica

    • Protocolo

    • Pureza y Documentación

    • Referencias

    • Revisión del cliente

    Descripciòn

    Fluo-4 AM is a cell-permeable Ca2+ (calcium ion) indicator (Ex/Em = 485/526 nm)[1].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)
    . 1. Solution preparation[1]
    1.1 Preparation of storage solution
    Solvent: DMSO
    Concentration: 1 mM is recommended (e.g. weigh 100 μg Fluo-4 AM and dissolve in 96.16 μL solvent).
    Storage: Use as soon as possible and avoid long-term storage.
    1.2 Preparation of working solution
    Dilute with buffer (e.g. HHBS containing 0.04% Poloxamer 407 (F127) (HY-D1005)) to 1 M (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately, and protected from light.

    2. Cell staining

    2.1 Count the cells and take 106 cells from each sample (control and experiment/s).
    2.2 Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.
    2.3 Resuspend the cells in PBS and add Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 °C for 1 h.
    2. 4 Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1 mL PBS.
    2.5 Evaluate the staining by flow cytometry (Ex/Em = 485/526 nm).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Fluo-4 AM Related Antibodies
    Peso molecular

    1096.94

    Fòrmula

    C51H50F2N2O23
    No. CAS
    Appearance

    Solid

    Color

    Brown to red

    Emission (Em)

    526
    Excitation (Ex)

    485
    SMILES

    O=C(OCOC(C)=O)CN(C1=CC=C(C2=C3C=C(F)C(C=C3OC4=C2C=C(F)C(OCOC(C)=O)=C4)=O)C=C1OCCOC5=CC(C)=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    -20°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvente y solubilidad
    In Vitro: 

    DMSO : 10 mg/mL (9.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9116 mL 4.5581 mL 9.1163 mL
    5 mM 0.1823 mL 0.9116 mL 1.8233 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Pureza y Documentación

    Purity: ≥99.0%

    SDS (252 KB)

    COA (255 KB)

    Instrucciones de manejo (2659 KB)
    Dyeing Example
    Referencias
    Ensayo celular
    [1]

    For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 μM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1].

    MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.9116 mL 4.5581 mL 9.1163 mL 22.7907 mL
    5 mM 0.1823 mL 0.9116 mL 1.8233 mL 4.5581 mL
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    Fluo-4 AM Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Fluo-4 AM273221-67-3Fluorescent DyeInhibitorinhibitorinhibit

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    Inquiry Information

    Nombre del producto:
    Fluo-4 AM
    Cat. No.:
    HY-101896
    Cantidad:
    MCE Japan Authorized Agent:

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