2. Others
  3. Fluorescent Dye
  4. Fluo-4 AM

Fluo-4 AM is a cell-permeable Ca2+ (calcium ion) indicator (Ex/Em = 485/526 nm).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 273221-67-3

容量 価格（税別） 在庫状況 数量
100 μg $150 在庫あり
500 μg $375 在庫あり
1 mg $525 在庫あり
5 mg $1600 在庫あり
10 mg $2650 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 56 publication(s) in Google Scholar

Other Forms of Fluo-4 AM:

Fluo-4 AM 関連抗体

Top Publications Citing Use of Products

顧客検証

IF

    Fluo-4 AM purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2022 May 17;13:855626.  [Abstract]

    Intracellular Ca2+ is measured by Fluo-4/AM (for 30 min) with flow cytometry.

    Fluo-4 AM purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Feb 10;2021:8884922.

    NP cells are incubated with 5 μM Fura-4-AM for 30 min at 37°C in the dark. Intracellular Ca2+ levels measured using the specific Ca2+-sensitive fluorescent indicator Fura-4-AM and analyzed via fluorescence microscopy.

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Fluo-4 AM is a cell-permeable Ca2+ (calcium ion) indicator (Ex/Em = 485/526 nm)[1].

    体外実験

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)
    . 1. Solution preparation[1]
    1.1 Preparation of storage solution
    Solvent: DMSO
    Concentration: 1 mM is recommended (e.g. weigh 100 μg Fluo-4 AM and dissolve in 96.16 μL solvent).
    Storage: Use as soon as possible and avoid long-term storage.
    1.2 Preparation of working solution
    Dilute with buffer (e.g. HHBS containing 0.04% Poloxamer 407 (F127) (HY-D1005)) to 1 M (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately, and protected from light.

    2. Cell staining

    2.1 Count the cells and take 106 cells from each sample (control and experiment/s).
    2.2 Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS.
    2.3 Resuspend the cells in PBS and add Fluo-4-AM (1 mM stock) to a final concentration of 1 μM. Incubate at 37 °C for 1 h.
    2. 4 Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1 mL PBS.
    2.5 Evaluate the staining by flow cytometry (Ex/Em = 485/526 nm).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Fluo-4 AM 関連抗体
    分子量

    1096.94

    分子式

    C51H50F2N2O23
    Emission (Em)

    526
    Excitation (Ex)

    485
    CAS 番号
    Appearance

    Solid

    Color

    Brown to red

    SMILES

    O=C(OCOC(C)=O)CN(C1=CC=C(C2=C3C=C(F)C(C=C3OC4=C2C=C(F)C(OCOC(C)=O)=C4)=O)C=C1OCCOC5=CC(C)=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.
    溶剤 & 溶解度
    体外: 

    DMSO : 10 mg/mL (9.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.9116 mL 4.5581 mL 9.1163 mL
    5 mM 0.1823 mL 0.9116 mL 1.8233 mL
    10 mM --- --- ---
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: ≥99.0%

    COA (255 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献
    細胞実験
    [1]

    For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 μM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.9116 mL 4.5581 mL 9.1163 mL 22.7907 mL
    5 mM 0.1823 mL 0.9116 mL 1.8233 mL 4.5581 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Fluo-4 AM Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Fluo-4 AM273221-67-3Fluorescent DyeInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Fluo-4 AM
    製品番号:
    HY-101896
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.