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  4. Fluorescamine

Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em: 390/475 nm). Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins.

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Fluorescamine

Fluorescamine 화학구조

CAS No. : 38183-12-9

사이즈 가격 재고 수량
무료 샘플 (0.1 - 0.2 mg)   지금 신청하기  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 해외재고보유
Solution
10 mM * 1 mL in DMSO 해외재고보유
Solid
10 mg 해외재고보유
50 mg 해외재고보유
100 mg 해외재고보유
500 mg 해외재고보유
1 g 해외재고보유
5 g   견적 받기  
10 g   견적 받기  

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고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of Fluorescamine:

Fluorescamine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Fluorescamine

  • Biological Activity

  • 순도&문서

  • References

  • 고객리뷰

제품 설명

Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em: 390/475 nm). Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Reagents and instruments
1.1 Fluorescamine solution: Dissolve Fluorescamine in acetone (recommended concentration 1.08 mM), prepare and use immediately (keep away from light).
1.2 Buffer: 0.05 M phosphate buffer (pH 8.0).
1.3 Standard protein solution: bovine serum albumin (BSA) or target protein, concentration gradient 0-1000 μg/mL.
1.3 Instrument: Fluorescence spectrophotometer (excitation wavelength 390 nm, emission wavelength 475 nm).
2. Operation steps and methods (standard curve method)
2.1 Sample preparation: Dilute the protein sample to be tested to the linear range (recommended 1-1000 μg/mL).
Blank control: buffer + Fluorescamine (no protein).
2.2 Reaction: Take 50 μL of sample or standard and add 150 μL buffer (pH 8.0). Then quickly add 50 μL Fluorescamine solution (96-well plate, shake and mix, react for 1-5 minutes).
2.3 Detection: Measure the fluorescence intensity immediately (delay may cause signal attenuation).
3. Data analysis
Use the fluorescence intensity (Y) of the standard protein to perform a linear regression on the concentration (X) to calculate the concentration of the sample to be tested.
4. Precautions
4.1 Please adjust the concentration of Fluorescamine working solution and incubation time according to actual conditions.
4.2 This product is limited to scientific research by professionals and shall not be used for clinical diagnosis or treatment, food or medicine.
4.3 For your safety and health, please wear a lab coat and disposable gloves during operation.
4.4 The reaction time must be consistent (it is recommended to be controlled within 2 minutes).
4.5 Avoid light operation. Fluorescamine is sensitive to light.
4.6 The reaction is best under alkaline conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescamine Related Antibodies
분자량

278.26

화학식

C17H10O4
CAS No.
Appearance

Solid

Color

White to light yellow

Emission (Em)

525
Excitation (Ex)

488
SMILES

O=C1C2(OC(C3=C2C=CC=C3)=O)OC=C1C4=CC=CC=C4
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도
In Vitro: 

DMSO : 100 mg/mL (359.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5938 mL 17.9688 mL 35.9376 mL
5 mM 0.7188 mL 3.5938 mL 7.1875 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • 몰농도 계산기

  • 농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
순도&문서

Purity: 98.0%

SDS (393 KB)

COA (256 KB) HNMR (97 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5938 mL 17.9688 mL 35.9376 mL 89.8440 mL
5 mM 0.7188 mL 3.5938 mL 7.1875 mL 17.9688 mL
10 mM 0.3594 mL 1.7969 mL 3.5938 mL 8.9844 mL
15 mM 0.2396 mL 1.1979 mL 2.3958 mL 5.9896 mL
20 mM 0.1797 mL 0.8984 mL 1.7969 mL 4.4922 mL
25 mM 0.1438 mL 0.7188 mL 1.4375 mL 3.5938 mL
30 mM 0.1198 mL 0.5990 mL 1.1979 mL 2.9948 mL
40 mM 0.0898 mL 0.4492 mL 0.8984 mL 2.2461 mL
50 mM 0.0719 mL 0.3594 mL 0.7188 mL 1.7969 mL
60 mM 0.0599 mL 0.2995 mL 0.5990 mL 1.4974 mL
80 mM 0.0449 mL 0.2246 mL 0.4492 mL 1.1231 mL
100 mM 0.0359 mL 0.1797 mL 0.3594 mL 0.8984 mL
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Fluorescamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescamine38183-12-9Ro 20-7234Fluorescent DyeInhibitorinhibitorinhibit

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상품명:
Fluorescamine
Cat. No.:
HY-D0715
수량:
MCE Japan Authorized Agent:

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SAFETY DATA SHEET (SDS)

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