38183-12-9
38183-12-9
Chemical Structure
Fluorescamine
Synonym(s): Ro 20-7234
- CAS No.: 38183-12-9
- Formula:C17H10O4
- Molecular Weight:278.26
IUPAC Name: 4-phenyl-3H,3'H-spiro[furan-2,1'-isobenzofuran]-3,3'-dione
InChIKey: ZFKJVJIDPQDDFY-UHFFFAOYSA-N
SMILES: O=C1C2(OC(C3=C2C=CC=C3)=O)OC=C1C4=CC=CC=C4
Biological Activity: Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em: 390/475 nm). Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins[1][2].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Fluorescamine | 98.0% | Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em: 390/475 nm). Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins. | ||||||||||||||||||||
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Fluorescamine (Standard) | ≥98% | Fluorescamine (Standard) is the analytical standard of Fluorescamine (HY-D0715). This product is intended for research and analytical applications. Fluorescamine is a spirocyclic compound that is non-fluorescent. Fluorescamine reacts rapidly with primary amine groups in proteins under alkaline conditions to generate products with strong fluorescence (Ex/Em : 390/475 nm). Fluorescamine can be used to detect amine-containing compounds, including amino acids, peptides, and proteins. | ||||||||||||||||||||
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References
- [1]. Udenfriend S, et al. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range. Science. 1972 Nov 24;178(4063):871-2. [Content Brief]
- [2]. Lorenzen A, et al. A fluorescence-based protein assay for use with a microplate reader. Anal Biochem. 1993 Oct;214(1):346-8. [Content Brief]
