1. Neuronal Signaling
  2. CaMK

KN-93 hydrochloride (Synonyms: KN 93 hydrochloride; KN93 hydrochloride)

Cat. No.: HY-15465A Purity: 99.67%
Handling Instructions

KN-93 hydrochloride is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

For research use only. We do not sell to patients.
KN-93 hydrochloride Chemical Structure

KN-93 hydrochloride Chemical Structure

CAS No. : 1956426-56-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 156 In-stock
1 mg USD 66 In-stock
5 mg USD 132 In-stock
10 mg USD 180 In-stock
25 mg USD 420 In-stock
50 mg USD 720 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Other Forms of KN-93 hydrochloride:

    KN-93 hydrochloride purchased from MCE. Usage Cited in: Exp Cell Res. 2018 Mar 15;364(2):198-207.

    Western blot analysis of β-catenin protein level in HEK 293T cells stimulated for 12h with rAGR2 (100 ng/mL) coupled with or without KN93 (10 μM).
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References


    KN-93 hydrochloride is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

    IC50 & Target

    Ki: 370 nM (CaMK-II)

    In Vitro

    After 2 days of KN-93 treatment, 95% of cells are arrested in G1. G1 arrest is reversible; 1 day after KN-93 release, a peak of cells had progressed into S and G2-M. KN-93 also blocks cell growth stimulated by basic fibroblast growth factor, platelet-derived growth factor-BB, epidermal growth factor, and insulin-like growth factor-1 in NIH 3T3 fibroblasts[1]. KN-93 inhibits the H+, K+-ATPase activity but strongly dissipates the proton gradient formed in the gastric membrane vesicles and reduces the volume of luminal space[2]. KN-93 (0.5 μM) prevents increased LV developed pressure during action potential prolongation and early afterdepolarizations. Ca2+-independent CaM kinase activity is increased during early afterdepolarizations and this increase is prevented by KN-93[3].

    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 1.8605 mL 9.3023 mL 18.6047 mL
    5 mM 0.3721 mL 1.8605 mL 3.7209 mL
    10 mM 0.1860 mL 0.9302 mL 1.8605 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay

    Cells are grown on 12-mm diameter glass coverslips in DMEM 100% serum and various concentrations of KN-93 or KN-92. After 0, 1, 2, and 3 days of culture in the presence of drug, coverslips are removed from culture, rinsed once in PBS, and then submerged in 100% methanol at -20°C for 3 min. Fixed cells are stored in PBS until staining using the TUNEL assay. Cells are overlaid on 20 μL PBS/1 mg/mL BSA for 30 min, rinsed in PBS, and then overlaid on 20 μL containing 100 mM sodium cacodylate (pH 6.8), 1 mM CoCl2, 0.1 mM DTT, 0.1 mg/mL BSA, 20 μM fluorescein-12-dUTP, and 0.1 unit/μL terminal transferase at 37°C for 60 min. Coverslips are rinsed in PBS twice, mounted on slides, and photographed using an OLYMPUS BX5O epifluorescent microscope using a UPLAN APO 40X oil immersion objective. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.




    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 31 mg/mL

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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