1. Neuronal Signaling
  2. CaMK

KN-93 phosphate 

Cat. No.: HY-15465B Purity: 99.91%
Handling Instructions

KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

For research use only. We do not sell to patients.

KN-93 phosphate Chemical Structure

KN-93 phosphate Chemical Structure

CAS No. : 1913269-12-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 174 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
1 mg USD 66 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 132 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 180 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg USD 720 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Other Forms of KN-93 phosphate:

    KN-93 phosphate purchased from MCE. Usage Cited in: Exp Cell Res. 2018 Mar 15;364(2):198-207.

    Western blot analysis of β-catenin protein level in HEK 293T cells stimulated for 12h with rAGR2 (100 ng/mL) coupled with or without KN93 (10 μM).

    KN-93 phosphate purchased from MCE. Usage Cited in: Front Pharmacol. 2018 Apr 12;9:362.

    The Simvastatin (SV)-increased biotinylated α7nAChR protein is blocked by the addition of KN93 (P<0.01, n=6 mice).

    KN-93 phosphate purchased from MCE. Usage Cited in: Diabetes. 2018 May 17. pii: db171521.

    Immunoblotting analysis of PCK1, G6Pase, and phospho-CaMKIIγ proteins in NTG and HCTG hepatocytes in response to PGF2α and in the presence or absence of KN-93 treatment.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

    IC50 & Target

    Ki: 370 nM (CaMK-II)

    In Vitro

    After 2 days of KN-93 treatment, 95% of cells are arrested in G1. G1 arrest is reversible; 1 day after KN-93 release, a peak of cells had progressed into S and G2-M. KN-93 also blocks cell growth stimulated by basic fibroblast growth factor, platelet-derived growth factor-BB, epidermal growth factor, and insulin-like growth factor-1 in NIH 3T3 fibroblasts[1]. KN-93 inhibits the H+, K+-ATPase activity but strongly dissipates the proton gradient formed in the gastric membrane vesicles and reduces the volume of luminal space[2]. KN-93 (0.5 μM) prevents increased LV developed pressure during action potential prolongation and early afterdepolarizations. Ca2+-independent CaM kinase activity is increased during early afterdepolarizations and this increase is prevented by KN-93[3].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 1.6694 mL 8.3468 mL 16.6937 mL
    5 mM 0.3339 mL 1.6694 mL 3.3387 mL
    10 mM 0.1669 mL 0.8347 mL 1.6694 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay
    [1]

    Cells are grown on 12-mm diameter glass coverslips in DMEM 100% serum and various concentrations of KN-93 or KN-92. After 0, 1, 2, and 3 days of culture in the presence of drug, coverslips are removed from culture, rinsed once in PBS, and then submerged in 100% methanol at -20°C for 3 min. Fixed cells are stored in PBS until staining using the TUNEL assay. Cells are overlaid on 20 μL PBS/1 mg/mL BSA for 30 min, rinsed in PBS, and then overlaid on 20 μL containing 100 mM sodium cacodylate (pH 6.8), 1 mM CoCl2, 0.1 mM DTT, 0.1 mg/mL BSA, 20 μM fluorescein-12-dUTP, and 0.1 unit/μL terminal transferase at 37°C for 60 min. Coverslips are rinsed in PBS twice, mounted on slides, and photographed using an OLYMPUS BX5O epifluorescent microscope using a UPLAN APO 40X oil immersion objective. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    599.03

    Formula

    C₂₆H₃₂ClN₂O₈PS

    CAS No.

    1913269-12-1

    SMILES

    OP(O)(O)=O.O=S(C1=CC=C(OC)C=C1)(N(C2=CC=CC=C2CN(C/C=C/C3=CC=C(Cl)C=C3)C)CCO)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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    Product Name:
    KN-93 phosphate
    Cat. No.:
    HY-15465B
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