1. Neuronal Signaling
  2. CaMK

KN-93 phosphate 

Cat. No.: HY-15465B Purity: 99.91%
Data Sheet SDS Handling Instructions

KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

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KN-93 phosphate Chemical Structure

KN-93 phosphate Chemical Structure

CAS No. : 1913269-12-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO $145 In-stock
1 mg $55 In-stock
5 mg $110 In-stock
10 mg $150 In-stock
50 mg $600 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Other Forms of KN-93 phosphate:

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

KN-93 phosphate is a novel membrane-permeant synthetic inhibitor of purified neuronal CaMK-II, with Ki of 370 nM.

IC50 & Target

Ki: 370 nM (CaMK-II)

In Vitro

After 2 days of KN-93 treatment, 95% of cells are arrested in G1. G1 arrest is reversible; 1 day after KN-93 release, a peak of cells had progressed into S and G2-M. KN-93 also blocks cell growth stimulated by basic fibroblast growth factor, platelet-derived growth factor-BB, epidermal growth factor, and insulin-like growth factor-1 in NIH 3T3 fibroblasts[1]. KN-93 inhibits the H+, K+-ATPase activity but strongly dissipates the proton gradient formed in the gastric membrane vesicles and reduces the volume of luminal space[2]. KN-93 (0.5 μM) prevents increased LV developed pressure during action potential prolongation and early afterdepolarizations. Ca2+-independent CaM kinase activity is increased during early afterdepolarizations and this increase is prevented by KN-93[3].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 1.6694 mL 8.3468 mL 16.6937 mL
5 mM 0.3339 mL 1.6694 mL 3.3387 mL
10 mM 0.1669 mL 0.8347 mL 1.6694 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay
[1]

Cells are grown on 12-mm diameter glass coverslips in DMEM 100% serum and various concentrations of KN-93 or KN-92. After 0, 1, 2, and 3 days of culture in the presence of drug, coverslips are removed from culture, rinsed once in PBS, and then submerged in 100% methanol at -20°C for 3 min. Fixed cells are stored in PBS until staining using the TUNEL assay. Cells are overlaid on 20 μL PBS/1 mg/mL BSA for 30 min, rinsed in PBS, and then overlaid on 20 μL containing 100 mM sodium cacodylate (pH 6.8), 1 mM CoCl2, 0.1 mM DTT, 0.1 mg/mL BSA, 20 μM fluorescein-12-dUTP, and 0.1 unit/μL terminal transferase at 37°C for 60 min. Coverslips are rinsed in PBS twice, mounted on slides, and photographed using an OLYMPUS BX5O epifluorescent microscope using a UPLAN APO 40X oil immersion objective. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

599.03

Formula

C₂₆H₃₂ClN₂O₈PS

CAS No.

1913269-12-1

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
KN-93 phosphate
Cat. No.:
HY-15465B
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