(E)-KN-93 phosphate
Based on 1 Customer Validation
(E)-KN-93 phosphate is a Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with IC50 values of 9 μM and 3 μM against CaMKIIδMonomer and CaMKIIδDodecamer, respectively. (E)-KN-93 phosphate can be used in research on epilepsy, depression, and arrhythmias..
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- Purity : 99.85%
- CAS No.: 1188890-41-6
- 화학식: C26H32ClN2O8PS
- 분자량:599.03
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보관:
RT, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
CaMK IIδ |
In Vitro
(E)-KN-93 phosphate exhibits IC50 values of 9 μM and 3 μM against CaMKIIδMonomer and CaMKIIδDodecamer, respectively[1].
(E)-KN-93 phosphate's binding to CaM depends on calcium ions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1188890-41-6
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Appearance Solid
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분자량 599.03
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화학식 C26H32ClN2O8PS
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Color White to off-white
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SMILES
O=P(O)(O)O.O=S(N(C1=C(C=CC=C1)CN(C)C/C=C/C2=CC=C(C=C2)Cl)CCO)(C3=CC=C(C=C3)OC)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
RT, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
용액&용해도
In Vitro:
DMSO : 100 mg/mL (166.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.17 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (271 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6694 mL | 8.3468 mL | 16.6937 mL | 41.7341 mL |
| 5 mM | 0.3339 mL | 1.6694 mL | 3.3387 mL | 8.3468 mL | |
| 10 mM | 0.1669 mL | 0.8347 mL | 1.6694 mL | 4.1734 mL | |
| 15 mM | 0.1113 mL | 0.5565 mL | 1.1129 mL | 2.7823 mL | |
| 20 mM | 0.0835 mL | 0.4173 mL | 0.8347 mL | 2.0867 mL | |
| 25 mM | 0.0668 mL | 0.3339 mL | 0.6677 mL | 1.6694 mL | |
| 30 mM | 0.0556 mL | 0.2782 mL | 0.5565 mL | 1.3911 mL | |
| 40 mM | 0.0417 mL | 0.2087 mL | 0.4173 mL | 1.0434 mL | |
| 50 mM | 0.0334 mL | 0.1669 mL | 0.3339 mL | 0.8347 mL | |
| 60 mM | 0.0278 mL | 0.1391 mL | 0.2782 mL | 0.6956 mL | |
| 80 mM | 0.0209 mL | 0.1043 mL | 0.2087 mL | 0.5217 mL | |
| 100 mM | 0.0167 mL | 0.0835 mL | 0.1669 mL | 0.4173 mL |
(E)-KN-93 phosphate Related Classifications
HY-110140 Related Classifications
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