LDC7559

Name: LDC7559
Brand: MedChemExpress
SKU: HY-111674
Rating: 5.0 (22 reviews)

Cat. No.: HY-111674 Pureza: 99.51%: Ficha de datos
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LDC7559 is a gasdermin D (GSDMD) inhibitor via blocking neutrophil extracellular trap (NET) in the late stages .

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 2407782-01-6

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 22 publication(s) in Google Scholar

22 Publications Citing Use of MCE LDC7559

Cell Imaging/Staining

Bio/Physico-chemical Assay

Cell Proliferation/Viability Assay

: LDC7559 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 May;21(5):934-956. [Abstract]; LDC7559 (5 μM; 2+24 h)did not prevent cell death induced by indatraline and sertraline. U2OS cells were preincubated with a specific inhibitor for pyroptosis, LDC7559 (5 μM), for 2 h and then treated with indatraline (10 μM) or sertraline (10 μM) for 24 h.

: LDC7559 purchased from MedChemExpress. Usage Cited in: Eur J Immunol. 2025 Apr;55(4):e202350942. [Abstract]; LDC7559 (2 µM) and stimulated for 1 h with extracellular Ca2+ (1 mM). LDC7559 blocked inflammasome‐mediated pore formation.

: LDC7559 purchased from MedChemExpress. Usage Cited in: Cancer Sci. 2025 Mar;116(3):673-689. [Abstract]; LDC7559 (10 μM; L； 72 h) inhibited Mdivi‐1‐induced cell death in both BCR::ABL1‐positive and BCR::ABL1‐negative cells. Cell viabilities, live cell counts, and dead cell ratios were evaluated after 72 h of Mdivi‐1 treatment with or without inhibitors. Inhibitors used included: Caspase inhibitor Z‐VAD‐FMK (Z); gasdermin D inhibitor LDC7559 (L); necroptosis inhibitor Necrostatin‐1 (N); ferroptosis inhibitor Ferrostatin‐1.

: LDC7559 purchased from MedChemExpress. Usage Cited in: Life Sci. 2023 Nov 1:332:122123. [Abstract]; LDC7559 (2.5 μM; 8 days) and disulfiram, attenuated VSMC calcification. Alizarin red staining of A7r5 cells in HP or control medium for 8 days treated with LDC7559 (2.5 μM), disulfiram (5 nM), or IL-1RA (5 μM) (scale bar 100 μm). The results were semi-quantified by the concentrations of Alizarin red.

: LDC7559 purchased from MedChemExpress. Usage Cited in: Life Sci. 2023 Nov 1:332:122123. [Abstract]; LDC7559 (2.5 μM; 8 days) treatment attenuated the cleavage of GSDMD and the release of LDHA in VSMCs subjected to a phosphate burden, indicating that pyroptosis was prevented. Immunoblots of A7r5 cell lysates and supernatants after 8 days of LDC7559 treatment.

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Descripciòn

LDC7559 is a gasdermin D (GSDMD) inhibitor via blocking neutrophil extracellular trap (NET) in the late stages ^[1].

IC₅₀ & Target

Gasdermin D^[1].

In Vitro

LDC7559 (1 and 5 μM) inhibits IL-1β release upon inflammasome activation, and significantly blocks the lethal effect of both human and murine GSDMD NT domains transfected into HEK293T cells^[3].
LDC7559 functions directly through blocking the activity of the GSDMD NT domain, rather than interfering with the activation and cleavage process^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

349.38

Fòrmula

C₂₀H₁₉N₃O₃

No. CAS

2407782-01-6

Appearance

Solid

Color

White to light yellow

SMILES

COC1=C(C=CC=C1)C2=NN3C(COC(C=C(NC(C)=O)C=C4)=C4C3)=C2

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (286.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8622 mL	14.3111 mL	28.6221 mL
5 mM	0.5724 mL	2.8622 mL	5.7244 mL
10 mM	0.2862 mL	1.4311 mL	2.8622 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.95 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.51%

Select Batch:

Ficha de datos (274 KB) SDS (251 KB)

COA (249 KB) HNMR (279 KB) LCMS (96 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Spel L, et al. Gasdermin D opens the way for NETs. Nat Rev Rheumatol. 2018 Dec;14(12):690-692. [Content Brief]

[2]. Sollberger G, et al. Gasdermin D plays a vital role in the generation of neutrophil extracellular traps. Sci Immunol. 2018 Aug 24;3(26). pii: eaar6689. [Content Brief]

[3]. Ankit Pandeya, et al. Gasdermin D (GSDMD) as a New Target for the Treatment of Infection. Medchemcomm. 2019 Apr 4;10(5):660-667. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8622 mL	14.3111 mL	28.6221 mL	71.5553 mL
	5 mM	0.5724 mL	2.8622 mL	5.7244 mL	14.3111 mL
	10 mM	0.2862 mL	1.4311 mL	2.8622 mL	7.1555 mL
	15 mM	0.1908 mL	0.9541 mL	1.9081 mL	4.7704 mL
	20 mM	0.1431 mL	0.7156 mL	1.4311 mL	3.5778 mL
	25 mM	0.1145 mL	0.5724 mL	1.1449 mL	2.8622 mL
	30 mM	0.0954 mL	0.4770 mL	0.9541 mL	2.3852 mL
	40 mM	0.0716 mL	0.3578 mL	0.7156 mL	1.7889 mL
	50 mM	0.0572 mL	0.2862 mL	0.5724 mL	1.4311 mL
	60 mM	0.0477 mL	0.2385 mL	0.4770 mL	1.1926 mL
	80 mM	0.0358 mL	0.1789 mL	0.3578 mL	0.8944 mL
	100 mM	0.0286 mL	0.1431 mL	0.2862 mL	0.7156 mL

LDC7559 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LDC75592407782-01-6LDC 7559LDC-7559PyroptosisInhibitorinhibitorinhibit

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LDC7559

Revisión del cliente

LDC7559 Related Antibodies

22 Publications Citing Use of MCE LDC7559

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

LDC7559 Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

LDC7559 Related Classifications

Keywords:

Productos vistos recientemente:

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