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  4. MQAE

MQAE is a chloride ion (Cl-) fluorescent probe that can be used to measure chloride concentrations. The fluorescence intensity of MQAE decreases proportionally as Cl- ions increase. MQAE has high cell permeability and is suitable for fluorescence detection such as confocal microscopy and flow cytometry (Ex/Em=350/460 nm).

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CAS 番号 : 162558-52-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
50 mg $60 在庫あり
100 mg $96 在庫あり
200 mg $156 在庫あり
500 mg $336 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 18 publication(s) in Google Scholar

MQAE 関連抗体

Top Publications Citing Use of Products

    MQAE purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Nov 16;22(1):1034.  [Abstract]

    Measurement of Cl− expression in HNEPCs using MQAE (5 mM, 1 h) (scale bar = 50 μm).

    MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2025 Oct 15;14(20):1601.  [Abstract]

    Stern–Volmer constant for MQAE (5 mM, 1 h).

    MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2025 Oct 15;14(20):1601.  [Abstract]

    Bars and dots (replicates) showed the quantification of MQAE (5 mM) fluorescence normalized to control in C38 cells.

    MQAE purchased from MedChemExpress. Usage Cited in: Front Nutr. 2021 Mar 29;8:638390.  [Abstract]

    Nile Red, MQAE, and CoroNa™ Green are used to stain the triglycerides (TG), chloride ions (Cl-), and sodium ions (Na+), respectively.

    MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2020 Apr 22;9(4):1045.  [Abstract]

    The concentration of cytoplasmic Cl− represented by the fluorescence of N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE, 5 Mm, 45 min).

    MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2020 Apr 22;9(4):1045.  [Abstract]

    The concentration of cytoplasmic Cl− represented by the fluorescence of N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE, 5 Mm, 45 min).

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    製品説明

    MQAE is a chloride ion (Cl-) fluorescent probe that can be used to measure chloride concentrations. The fluorescence intensity of MQAE decreases proportionally as Cl- ions increase. MQAE has high cell permeability and is suitable for fluorescence detection such as confocal microscopy and flow cytometry (Ex/Em=350/460 nm)[1][2].

    体外実験

    Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
    1 Preparation of MQAE working solution
    1.1 Preparation of the stock solution
    Dissolve 1 mg MQAE in 0.3066 mL DMSO to obtain 10 mM of MQAE.
    Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of MQAE working solution
    Dilute the stock solution in Krebs-hepes buffer (20 mM HEPES, 128 mM NaCl, 2.5 mM KCl, 2.7 mM CaCl2, 1 mM MgCl2, 16 mM glucose, pH 7.4) to obtain 5-10 mM of MQAE working solution.
    Note: Please adjust the concentration of MQAE working solution according to the actual situation.

    2 Cell staining
    2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    2.2 Add 1 mL of MQAE working solution, and then incubate at room temperature for 30 minutes.
    2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS.

    3 Storage
    -20°C, 1 year. Protect from light.
    4 Precautions
    4.1 It is recommended to store the stock solution at -20°C or -80°Caway from light and avoid repetitive freeze-thaw cycles.
    4.2 Please adjust the concentration of MQAE working solution according to the actual situation.
    4.3 This product is for R&D use only, not for drug, household, or other uses.
    4.4 For your safety and health, please wear a lab coat and disposable gloves to operate.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    MQAE 関連抗体
    分子量

    326.19

    分子式

    C14H16BrNO3
    Emission (Em)

    460
    Excitation (Ex)

    355
    CAS 番号
    Appearance

    Solid

    Color

    Light yellow to khaki

    SMILES

    COC1=CC2=CC=C[N+](CC(OCC)=O)=C2C=C1.[Br-]
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    H2O : 100 mg/mL (306.57 mM; Need ultrasonic)

    DMSO : ≥ 35 mg/mL (107.30 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0657 mL 15.3285 mL 30.6570 mL
    5 mM 0.6131 mL 3.0657 mL 6.1314 mL
    10 mM 0.3066 mL 1.5328 mL 3.0657 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.42%

    COA (248 KB) HNMR (240 KB) RP-HPLC (248 KB) LCMS (96 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.0657 mL 15.3285 mL 30.6570 mL 76.6424 mL
    5 mM 0.6131 mL 3.0657 mL 6.1314 mL 15.3285 mL
    10 mM 0.3066 mL 1.5328 mL 3.0657 mL 7.6642 mL
    15 mM 0.2044 mL 1.0219 mL 2.0438 mL 5.1095 mL
    20 mM 0.1533 mL 0.7664 mL 1.5328 mL 3.8321 mL
    25 mM 0.1226 mL 0.6131 mL 1.2263 mL 3.0657 mL
    30 mM 0.1022 mL 0.5109 mL 1.0219 mL 2.5547 mL
    40 mM 0.0766 mL 0.3832 mL 0.7664 mL 1.9161 mL
    50 mM 0.0613 mL 0.3066 mL 0.6131 mL 1.5328 mL
    60 mM 0.0511 mL 0.2555 mL 0.5109 mL 1.2774 mL
    80 mM 0.0383 mL 0.1916 mL 0.3832 mL 0.9580 mL
    100 mM 0.0307 mL 0.1533 mL 0.3066 mL 0.7664 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    MQAE Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    MQAE162558-52-3Fluorescent DyeInhibitorinhibitorinhibit

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    製品名:
    MQAE
    製品番号:
    HY-D0090
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