Para-aminoblebbistatin

Name: Para-aminoblebbistatin
Brand: MedChemExpress
SKU: HY-111474
Rating: 4.5 (0 reviews)

Cat. No.: HY-111474 Purity: 99.9%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

Para-aminoblebbistatin is the inhibitor for myosin II. Para-aminoblebbistatin inhibits the ATPase activity of myosin II, thereby blocking the formation and contraction of the actin-myosin network. Para-aminoblebbistatin is a non-fluorescent and photostable Blebbistatin (HY-13813) derivative.

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Para-aminoblebbistatin 화학구조

CAS No. : 2097734-03-5

사이즈	가격	재고	수량
500 μg		해외재고보유	Estimated Time of Arrival: December 31

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

Myosin II^[1]

In Vitro

Para-aminoblebbistatin inhibits multiple myosin II isoforms, including skeletal muscle, cardiac muscle, non-muscle myosin II. Para-aminoblebbistatin inhibits rabbit skeletal muscle myosin S1 (SkS1) and dictyostelium discoideum myosin II motor domain (DdMD) with IC₅₀ of 1.3 μM and 6.6 μM^[1].
Para-aminoblebbistatin (0-50 μM, 72 h) inhibits the proliferation of HeLa cell with an IC₅₀ of 17.8 μM, promotes the cell migration and inhibits the blebbing process of M2 cell^[1].
Para-aminoblebbistatin (2-20 μM, 10 stimulations every hour for 4 hours) inhibits the rapid escape response (C-start reflex) of zebrafish embryo, reduces the amplitude of zebrafish embryo myocardial contraction^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	HeLa
Concentration:	0-50 μM
Incubation Time:	3 days
Result:	Reduced the cell number.

Cell Migration Assay ^[1]

Cell Line:	HeLa
Concentration:	20 μM
Incubation Time:	24 h
Result:	Promoted the cell migration.

In Vivo

Para-aminoblebbistatin (16 mg/kg, ip, single dose) causes death of Wistar rats due to heart failure^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wistar rats models^[3]
Dosage:	16 mg/kg
Administration:	ip, single dose
Result:	Killed rats in 15 minutes.

분자량

307.35

화학식

C₁₈H₁₇N₃O₂

CAS No.

2097734-03-5

Appearance

Solid

Color

Orange to red

SMILES

O=C1[C@@]2(O)C(N(C3=CC=C(N)C=C3)CC2)=NC4=CC=C(C)C=C41

선적

Room temperature in continental US; may vary elsewhere.

보관

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

용액&용해도

In Vitro:

DMF : 20 mg/mL (65.07 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2536 mL	16.2681 mL	32.5362 mL
5 mM	0.6507 mL	3.2536 mL	6.5072 mL
10 mM	0.3254 mL	1.6268 mL	3.2536 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.9%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (256 KB)

Handling Instructions (2659 KB)

References

[1]. Várkuti BH, et al. A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci Rep. 2016 May 31;6:26141. [Content Brief]

[2]. Képiró M, et al. para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor. Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5. [Content Brief]

[3]. Gyimesi M, et al., Single Residue Variation in Skeletal Muscle Myosin Enables Direct and Selective Drug Targeting for Spasticity and Muscle Stiffness. Cell. 2020 Oct 15;183(2):335-346.e13. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF	1 mM	3.2536 mL	16.2681 mL	32.5362 mL	81.3405 mL
	5 mM	0.6507 mL	3.2536 mL	6.5072 mL	16.2681 mL
	10 mM	0.3254 mL	1.6268 mL	3.2536 mL	8.1340 mL
	15 mM	0.2169 mL	1.0845 mL	2.1691 mL	5.4227 mL
	20 mM	0.1627 mL	0.8134 mL	1.6268 mL	4.0670 mL
	25 mM	0.1301 mL	0.6507 mL	1.3014 mL	3.2536 mL
	30 mM	0.1085 mL	0.5423 mL	1.0845 mL	2.7113 mL
	40 mM	0.0813 mL	0.4067 mL	0.8134 mL	2.0335 mL
	50 mM	0.0651 mL	0.3254 mL	0.6507 mL	1.6268 mL
	60 mM	0.0542 mL	0.2711 mL	0.5423 mL	1.3557 mL