1. Anti-infection
  2. Bacterial
  3. Resazurin sodium

Resazurin sodium (Synonyms: Diazoresorcinol sodium)

Cat. No.: HY-111391 Purity: ≥98.0%
Handling Instructions

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.

For research use only. We do not sell to patients.

Resazurin sodium Chemical Structure

Resazurin sodium Chemical Structure

CAS No. : 62758-13-8

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Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
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ready for reconstitution
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Estimated Time of Arrival: December 31
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1 g USD 60 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Resazurin sodium

  • Biological Activity

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  • References

  • Customer Review

Description

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.

In Vitro

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin. No viable bacteria are detected 24 h post-inoculation following inclusion of Resazurin sodium in TSBc cultures of F. tularensis LVS at the concentration of 44 μM. Lowering the Resazurin sodium concentration to as little as 4.4 μM still results in a 10-fold reduction in viable F. tularensis LVS compare to growth medium alone. Both Resazurin sodium treatments result in a significant decrease in viable F. tularensis LVS bacteria over 22 h. Treatment with Resazurin sodium significantly reduces the number of viable F. tularensis LVS bacteria in HEK293 cells 22 h post-infection[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

251.17

Formula

C12H6NNaO4

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (331.77 mM; Need ultrasonic)

H2O : 5 mg/mL (19.91 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9814 mL 19.9068 mL 39.8137 mL
5 mM 0.7963 mL 3.9814 mL 7.9627 mL
10 mM 0.3981 mL 1.9907 mL 3.9814 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

*All of the co-solvents are available by MCE.
References
Cell Assay
[1]

F. tularensis is cultured in TSBc supplemented with 44 μM Resazurin sodium salt at 37°C with shaking for 24 h. At select time points, a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600 nm and 570 nm to detect the presence of Resazurin sodium salt and resorufin, respectively. The ratio of these two optical densities is used to evaluate reduction of Resazurin sodium salt to resorufin over time[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: ≥98.0%

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Product Name:
Resazurin sodium
Cat. No.:
HY-111391
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