2. Anti-infection
  3. Bacterial
  4. Resazurin sodium

Resazurin sodium  (Synonyms: Diazoresorcinol sodium)

Cat. No.: HY-111391 Purity: ≥98.0%
COA Handling Instructions

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.

For research use only. We do not sell to patients.

Resazurin sodium Chemical Structure

Resazurin sodium Chemical Structure

CAS No. : 62758-13-8

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10 mM * 1 mL in DMSO
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Based on 4 publication(s) in Google Scholar

Other Forms of Resazurin sodium:

Resazurin sodium Related Antibodies

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Description

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.

In Vitro

Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin. No viable bacteria are detected 24 h post-inoculation following inclusion of Resazurin sodium in TSBc cultures of F. tularensis LVS at the concentration of 44 μM. Lowering the Resazurin sodium concentration to as little as 4.4 μM still results in a 10-fold reduction in viable F. tularensis LVS compare to growth medium alone. Both Resazurin sodium treatments result in a significant decrease in viable F. tularensis LVS bacteria over 22 h. Treatment with Resazurin sodium significantly reduces the number of viable F. tularensis LVS bacteria in HEK293 cells 22 h post-infection[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Resazurin sodium Related Antibodies
Molecular Weight

251.17

Appearance

Solid

Formula

C12H6NNaO4
CAS No.
SMILES

O=C1C=CC2=[N+]([O-])C3=C(C=C(O[Na])C=C3)OC2=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 5 mg/mL (19.91 mM; ultrasonic and warming and heat to 60°C)

DMSO : 2 mg/mL (7.96 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9814 mL 19.9068 mL 39.8137 mL
5 mM 0.7963 mL 3.9814 mL 7.9627 mL
10 mM 0.3981 mL 1.9907 mL 3.9814 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (8.28 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (248 KB) HNMR (282 KB) MS (68 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

F. tularensis is cultured in TSBc supplemented with 44 μM Resazurin sodium salt at 37°C with shaking for 24 h. At select time points, a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600 nm and 570 nm to detect the presence of Resazurin sodium salt and resorufin, respectively. The ratio of these two optical densities is used to evaluate reduction of Resazurin sodium salt to resorufin over time[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Resazurin sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Keywords:

Resazurin62758-13-8DiazoresorcinolBacterialInhibitorinhibitorinhibit

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