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  3. Fluorescent Dye
  4. Resazurin

Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal).

For research use only. We do not sell to patients.

Resazurin Chemical Structure

Resazurin Chemical Structure

CAS No. : 550-82-3

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Resazurin Related Antibodies

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Description

Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal)[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability[1][2]:
1. Defrost the Resazurin solution in a 37°C water bath.
2. Place the cells on a 96-well plate, and washed by PBS (avoid light).
3. Remove the PBS wash, then add 500 μL (1μg/mL) of Resazurin solution.
4. Place the plate, in the incubator for 30 min (incubation time depends on cell type and cell number).
5. Measure Resorufin fluorescence (Ex=530-560 nm, Em=590 nm) using a microplate reader.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Resazurin Related Antibodies
Molecular Weight

229.19

Formula

C12H7NO4
CAS No.
Emission (Em)

590
Excitation (Ex)

530/560
SMILES

O=C1C=CC2=[N+](C3=C(OC2=C1)C=C(C=C3)O)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Resazurin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Resazurin550-82-3DiazoresorcinolFluorescent Dyecell viability assaycytotoxicityResorufincell proliferation assayInhibitorinhibitorinhibit

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