1. Membrane Transporter/Ion Channel
  2. Proton Pump
  3. Soraprazan

Soraprazan (Synonyms: BYK61359)

Cat. No.: HY-100414 Purity: 99.02%
Handling Instructions

Soraprazan is a reversible, and fast-acting inhibitor of gastric H+/K+ ATPase.

For research use only. We do not sell to patients.

Soraprazan Chemical Structure

Soraprazan Chemical Structure

CAS No. : 261944-46-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 437 In-stock
Estimated Time of Arrival: December 31
1 mg USD 180 In-stock
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5 mg USD 540 In-stock
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10 mg USD 900 In-stock
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25 mg USD 1800 In-stock
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50 mg USD 2520 In-stock
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100 mg USD 3960 In-stock
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Description

Soraprazan is a reversible, and fast-acting inhibitor of gastric H+/K+ ATPase.

In Vitro

Soraprazan is a potent inhibitor of gastric H,K-ATPase, with an IC50 of 0.1 μM when measured in ion-leaky vesicles in the presence of 1 mM potassium. Soraprazan also effectively inhibits dibutyryl cAMP-stimulated [14C]AP accumulation in isolated gastric glands with an IC50 of 0.19 μM (0.09-0.40 μM geometric mean from n=6 with 95% confidence limits). In ion-leaky vesicles, soraprazan is a potent k-competitive inhibitor of the H,K-ATPase, with Ki of 6.4 nM. Soraprazan binds to the H,K-ATPase in ion-leaky vesicles with a Kd of 26.4 nM and a Bmax of 2.89 nmol/mg[1].

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 150 mg/mL (408.23 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7215 mL 13.6077 mL 27.2153 mL
5 mM 0.5443 mL 2.7215 mL 5.4431 mL
10 mM 0.2722 mL 1.3608 mL 2.7215 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

[3H]Soraprazan binding studies are carried out at 20°C. In saturation experiments to determine the Kd value, ion-leaky gastric vesicles (0.01-0.02 mg/mL) are resuspended in a buffer composed of 20 mM Tris-HCl, pH 7.0, 2 mM MgCl2, and 2 mM ATP (pH 7.0 by Tris) in the presence of increasing concentrations of [3H]soraprazan (0.1 nM-1 μM). Nonspecific binding is determined in the presence of a 100 fold excess of unlabeled soraprazan over the concentration range of [3H]soraprazan used. The enzyme suspension (1 mL) is incubated at 20°C for 30 min and rapidly filtered through a nitrocellulose membrane filter (0.45 μM) prewet with a solution composed of 20 mM Tris-HCl, pH 7.0, 10% polyethylene glycol 3350 that is placed on top of a glass fiber filter. The membrane is ished five times with 2.5 mL of a buffer composed of 20 mM Tris-HCl, pH 7.0, and 10% polyethylene glycol 3350 to remove unbound inhibitor. The membrane is put into a 20-mL scintillation vial, dimethylacetamide (0.5 mL) is added to dissolve the membrane, and 14 mL of scintillation solvent is added and counted. Binding of [3H]soraprazan is determined by subtracting the nonspecific binding of [3H]soraprazan, obtained in the presence of the 100-fold excess of nonradioactive soraprazan, from the amounts of [3H]soraprazan bound to the membrane in the absence of the cold inhibitor.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

367.44

Formula

C₂₁H₂₅N₃O₃

CAS No.

261944-46-1

SMILES

O[[email protected]@H]1[[email protected]@H](C2=CC=CC=C2)NC3=C(C=CN4C3=NC(C)=C4C)[[email protected]]1OCCOC

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Room temperature in continental US; may vary elsewhere

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Product Name:
Soraprazan
Cat. No.:
HY-100414
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Soraprazan

Cat. No.: HY-100414