2. Membrane Transporter/Ion Channel
  3. Proton Pump
  4. Soraprazan

Soraprazan  (Synonyms: BYK61359)

Cat. No.: HY-100414 Purity: 99.54%
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Soraprazan (BYK61359) is a selective, reversible K-competitive inhibitor of the H,K-ATPase (Ki=6.4 nM), with an IC50 of 0.19 μM in gastric glands. Soraprazan binds to the H,K-ATPase with a Kd of 28.27 nM. Soraprazan shows immediate inhibition of acid secretion and is more than 2000-fold selective for H,K-ATPase over Na,K- and Ca-ATPases.

For research use only. We do not sell to patients.

Soraprazan Chemical Structure

Soraprazan Chemical Structure

CAS No. : 261944-46-1

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 437 In-stock
Solution
10 mM * 1 mL in DMSO USD 437 In-stock
Solid
1 mg USD 180 In-stock
5 mg USD 540 In-stock
10 mg USD 900 In-stock
25 mg USD 1800 In-stock
50 mg USD 2520 In-stock
100 mg USD 3960 In-stock
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Based on 1 publication(s) in Google Scholar

Soraprazan Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Soraprazan

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Soraprazan (BYK61359) is a selective, reversible K-competitive inhibitor of the H,K-ATPase (Ki=6.4 nM), with an IC50 of 0.19 μM in gastric glands. Soraprazan binds to the H,K-ATPase with a Kd of 28.27 nM. Soraprazan shows immediate inhibition of acid secretion and is more than 2000-fold selective for H,K-ATPase over Na,K- and Ca-ATPases[1].

In Vitro

Soraprazan (BYK61359) is a potent inhibitor of gastric H,K-ATPase, with an IC50 of 0.1 μM when measured in ion-leaky vesicles in the presence of 1 mM potassium. Soraprazan (BYK61359) also effectively inhibits dibutyryl cAMP-stimulated [14C]AP accumulation in isolated gastric glands with an IC50 of 0.19 μM (0.09-0.40 μM geometric mean from n=6 with 95% confidence limits)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Soraprazan Related Antibodies
In Vivo

Soraprazan (1-27 μmol/kg; p.o.) shows rapid and consistent inhibition of acid secretion in dog [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

367.44

Formula

C21H25N3O3
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O[C@@H]1[C@@H](C2=CC=CC=C2)NC3=C(C=CN4C3=NC(C)=C4C)[C@H]1OCCOC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (544.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7215 mL 13.6077 mL 27.2153 mL
5 mM 0.5443 mL 2.7215 mL 5.4431 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (13.61 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Kinase Assay
[1]

[3H]Soraprazan binding studies are carried out at 20°C. In saturation experiments to determine the Kd value, ion-leaky gastric vesicles (0.01-0.02 mg/mL) are resuspended in a buffer composed of 20 mM Tris-HCl, pH 7.0, 2 mM MgCl2, and 2 mM ATP (pH 7.0 by Tris) in the presence of increasing concentrations of [3H]soraprazan (0.1 nM-1 μM). Nonspecific binding is determined in the presence of a 100 fold excess of unlabeled soraprazan over the concentration range of [3H]soraprazan used. The enzyme suspension (1 mL) is incubated at 20°C for 30 min and rapidly filtered through a nitrocellulose membrane filter (0.45 μM) prewet with a solution composed of 20 mM Tris-HCl, pH 7.0, 10% polyethylene glycol 3350 that is placed on top of a glass fiber filter. The membrane is ished five times with 2.5 mL of a buffer composed of 20 mM Tris-HCl, pH 7.0, and 10% polyethylene glycol 3350 to remove unbound inhibitor. The membrane is put into a 20-mL scintillation vial, dimethylacetamide (0.5 mL) is added to dissolve the membrane, and 14 mL of scintillation solvent is added and counted. Binding of [3H]soraprazan is determined by subtracting the nonspecific binding of [3H]soraprazan, obtained in the presence of the 100-fold excess of nonradioactive soraprazan, from the amounts of [3H]soraprazan bound to the membrane in the absence of the cold inhibitor.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7215 mL 13.6077 mL 27.2153 mL 68.0383 mL
5 mM 0.5443 mL 2.7215 mL 5.4431 mL 13.6077 mL
10 mM 0.2722 mL 1.3608 mL 2.7215 mL 6.8038 mL
15 mM 0.1814 mL 0.9072 mL 1.8144 mL 4.5359 mL
20 mM 0.1361 mL 0.6804 mL 1.3608 mL 3.4019 mL
25 mM 0.1089 mL 0.5443 mL 1.0886 mL 2.7215 mL
30 mM 0.0907 mL 0.4536 mL 0.9072 mL 2.2679 mL
40 mM 0.0680 mL 0.3402 mL 0.6804 mL 1.7010 mL
50 mM 0.0544 mL 0.2722 mL 0.5443 mL 1.3608 mL
60 mM 0.0454 mL 0.2268 mL 0.4536 mL 1.1340 mL
80 mM 0.0340 mL 0.1701 mL 0.3402 mL 0.8505 mL
100 mM 0.0272 mL 0.1361 mL 0.2722 mL 0.6804 mL
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Soraprazan Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Soraprazan261944-46-1BYK61359BYK 61359BYK-61359Proton PumpgastricglandssecretioncompetitiveInhibitorinhibitorinhibit

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