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  4. TMA-DPH

TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm). TMA-DPH is able to anchor on the cell surface and localize to different regions of the phospholipid bilayer. By analyzing the fluorescence polarization values of TMA-DPH in the plasma membrane and membrane substructures, the fluidity of the cell membrane can be determined.

For research use only. We do not sell to patients.

CAS No. : 115534-33-3

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Based on 11 publication(s) in Google Scholar

Other Forms of TMA-DPH:

TMA-DPH Related Antibodies

Top Publications Citing Use of Products

    TMA-DPH purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 Dec 11;68(23):25307-25323.  [Abstract]

    Membrane fluidity quantification using TMA-DPH fluorescence (24 h; n = 6).

    TMA-DPH purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2024 Jan 8;15(1):12.  [Abstract]

    Quantification of mitochondrial membrane fluidity of L-ADSCs and O-ADSCs by the fluorescence polarization (P) values of mitochondrial membrane measured by TMA-DPH (5 μM) dye. P value and membrane fluidity are negative correction (n ≥ 3).

    TMA-DPH purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Jan 1:249:114375.  [Abstract]

    The cell membrane fluidity was evaluated by TMA-DPH (0.5 μM, at 37 °C for 20 min).

    TMA-DPH purchased from MedChemExpress. Usage Cited in: Front Pharmacol. 2021 May 28:12:663743.  [Abstract]

    The changes in the fluorescence anisotropy of TMA-DPH (10 μM, at 37°C in dark for 20 min) in Caco-2 cells with different concentrations of benzaldehyde molecules. (●) HBSS, (■) 25 μM benzaldehyde, (▲) 100 μM benzaldehyde. Data are shown as the mean ± SD.

    TMA-DPH purchased from MedChemExpress. Usage Cited in: Biotechnol Biofuels. 2019 Mar 19:12:59.  [Abstract]

    Membrane fluidity of the RHA strains of DAP1 at high temperature. The Membrane fluidity is determined by the steady-state anisotropy of fluorescent probe 1-[4-(trimethylamino)pheny]-6-phenyl-1,3,5-hexatriene (TMA-DPH, 42 °C).

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm). TMA-DPH is able to anchor on the cell surface and localize to different regions of the phospholipid bilayer. By analyzing the fluorescence polarization values of TMA-DPH in the plasma membrane and membrane substructures, the fluidity of the cell membrane can be determined[1][2][3].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Solution preparation
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 10 mM (optimized according to the experiment).
    1.2 Preparation of working solution
    Dilute to 0.5-5 μM with PBS or serum-free medium (pH 7.4) (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining (suspended cells)
    2.1 Collect cells by centrifugation and wash twice with PBS for 5 minutes each time.
    2.2 TMA-DPH (0.5-5 μM) in Hanks and 20 mM Hepes buffer pH 7.4, and incubate it at 37°C, for 5-30 minutes (optimized according to the experiment).
    2.3 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
    2.4 Add PBS to wash the cells twice, 5 minutes each time.
    2.5 Wash the cells and resuspended in the appropriate Hepes buffer pH 7.4.
    2.6 Observe using a fluorescence microscope or flow cytometer.
    3. Cell staining (adherent cells)
    3.1 Culture the adherent cells on a sterile coverslip.
    3.2 Remove the coverslip from the culture medium and remove the excess culture medium.
    3.3 Add TMA-DPH (0.5-5 μM) in Hanks and 20 mM Hepes buffer pH 7.4, and incubate it at 37°C, for 5-30 minutes (optimized according to the experiment).
    3.4 Wash the cells and resuspended in the appropriate Hepes buffer pH 7.4.
    3.5 Fluorescence microscopy detection (Ex/Em = 355/430 nm).
    Note: If flow cytometry is required, the cells need to be digested with trypsin and resuspended before staining.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TMA-DPH Related Antibodies
    Molecular Weight

    461.62

    Formula

    C28H31NO3S
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    Emission (Em)

    430
    Excitation (Ex)

    355
    SMILES

    C[N+](C)(C)C1=CC=C(/C=C/C=C/C=C/C2=CC=CC=C2)C=C1.[O-]S(=O)(C3=CC=C(C)C=C3)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 10 mg/mL (21.66 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1663 mL 10.8314 mL 21.6628 mL
    5 mM 0.4333 mL 2.1663 mL 4.3326 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.86%

    SDS (393 KB)

    COA (249 KB) HNMR (269 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    L929 cells in 2 mL DM10F are allowed to adhere on microscope slide flasks. For the observation of plasma membrane labeling, cells are incubated for a short time (10 s) at room temperature with TMA-DPH 2×10-6 M in PBS or in DM10F from a 4×10-3 M stock solution in dimethylformamide. The unwashed slide is then transferred to the microscope and observed. For the labeling of internalized membrane, the cells are incubated in slide flasks at 37°C, with TMA-DPH 2×10-6 M in DM10F for the desired time, and then washed by gently shaking the slide in PBS for a few seconds[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1663 mL 10.8314 mL 21.6628 mL 54.1571 mL
    5 mM 0.4333 mL 2.1663 mL 4.3326 mL 10.8314 mL
    10 mM 0.2166 mL 1.0831 mL 2.1663 mL 5.4157 mL
    15 mM 0.1444 mL 0.7221 mL 1.4442 mL 3.6105 mL
    20 mM 0.1083 mL 0.5416 mL 1.0831 mL 2.7079 mL
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    TMA-DPH Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TMA-DPH115534-33-3Fluorescent DyeInhibitorinhibitorinhibit

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