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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. 상품명 효과 Purity Chemical Structure
  • HY-137798
    Chromozym PL
    		99.18%
    Chromozym PL is a chromogenic substrate for plasmin, and the enzymatic reaction can be carried out in 0.1mL Tris-HCl buffer (50 mM, pH 7.8). 100 μM Chromozym PL was dissolved and prepared. After adding the hydrolase, the generation of p-nitroaniline (pNA) at 405 nm was continuously observed, and the hydrolysis products were calculated.
    Chromozym PL
  • HY-D0281
    Pigment Yellow 14
    Pigment Yellow 14 is a fluorescent dye.
    Pigment Yellow 14
  • HY-D1798
    Vari Fluor 350 SE
    Vari Fluor 350 SE (VF 350 SE) is a dye marker of the Vari Fluor SE series (Ex/Em=350 nm/448 nm). The Vari Fluor SE series of dyes are a class of fluorescent dyes containing NHS ester groups used to label free amines (-NHX) on antibodies, proteins, peptides, amine-modified oligonucleotides and other biomolecules.
    Vari Fluor 350 SE
  • HY-128536
    KMG-104AM
    KMG-104AM is a Mg2+ fluorescent probe. KMG-104AM is a membrane-permeable ester-modified derivative of KMG-104, which serves as a reporter and imaging agent. KMG-104AM can track the increase in intracellular free magnesium ion concentration induced by mitochondrial uncoupling. KMG-104AM enables visualization of the three-dimensional distribution of intracellular magnesium ion concentration. KMG-104AM is applicable to research related to the functions of intracellular magnesium ions.
    KMG-104AM
  • HY-P4419
    Boc-Asp(OBzl)-Pro-Arg-AMC
    Boc-Asp(OBzl)-Pro-Arg-AMC is a fluorogenic substrate for the determination of protease activity. Boc-Asp(OBzl)-Pro-Arg-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths are 351 nm and 430 nm, respectively.
    Boc-Asp(OBzl)-Pro-Arg-AMC
  • HY-168378A
    D-Val-Leu-Lys-pNA acetate
    D-Val-Leu-Lys-pNA acetate (S-2251 acetate) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA acetate acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA acetate binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity.
    D-Val-Leu-Lys-pNA acetate
  • HY-D1478
    Fluorescent polyamine probe-1
    Fluorescent polyamine probe-1 (compound 15) is a linear polyamine probe with high uptake efficiency. Fluorescent polyamine probe-1 can be used for the research of transport system into cancer cells.
    Fluorescent polyamine probe-1
  • HY-D2373
    CY5.5-PEG2000-Mal
    CY5.5-PEG2000-Mal is used for preparing fluorescently labeled molecules. It is formed by condensing maleimide groups (Mal), PEG2000, and fluorescent dye CY5.5 (HY-D0924).
    CY5.5-PEG2000-Mal
  • HY-179074D
    FITC-Polysucrose 100
    FITC-Polysucrose 100 is FITC-labeled Polysucrose 100. FITC-Polysucrose 100 is mainly used for permeability and microcirculation studies (Ex/Em = 488/525 nm).
    FITC-Polysucrose 100
  • HY-D2002
    ATTO 488 maleimide
    ATTO 488 maleimide is a thiol-reactive fluorescent labeling reagent that forms a covalent bond via its maleimide group with the thiol group of the C-terminal cysteine residue of α-synGC. ATTO 488 maleimide enables α-syn fiber imaging via super-resolution stimulated emission depletion (STED) fluorescence microscopy. ATTO 488 maleimide is applicable to research related to Parkinson's disease (Ex/Em = 488/592nm).
    ATTO 488 maleimide
  • HY-W013430
    4-Amino-3-hydroxynaphthalene-1-sulfonic acid
    		98.0%
    4-Amino-3-hydroxynaphthalene-1-sulfonic acid is utilized as a fluorescent dye. 4-Amino-3-hydroxynaphthalene-1-sulfonic acid forms polypyrrole (PPy) conducting film with pyrrole, and is utilized in electrochemie.
    4-Amino-3-hydroxynaphthalene-1-sulfonic acid
  • HY-149203
    MQA-P
    MQA-P is a multifunctional near-infrared (NIR) fluorescent probe for simultaneously detecting ONOO-, viscosity, and polarity within mitochondria. MQA-P exhibits a remarkable turn-on response to ONOO- (λem=645 nm) and is highly sensitive to viscosity/polarity in the NIR channel with λem>704 nm. MQA-P exhibits excited-state intramolecular charge transfer (ESICT) feature that is highly polarity-sensitive by engineering N,N-dimethylamino as the electron donor and a quinoline cationic unit as the electron acceptor. MQA-P is used for ferroptosis or cancer diagnosis in vitro and in vivo via dual-channel images.
    MQA-P
  • HY-D2569
    Cy5.5-PEG2000-Biotin
    Cy5.5-PEG2000-Biotin is a polyethylene glycol derivative containing Cy5.5 (HY-D0924) fluorescent dye and polyethylene glycol (PEG) and a Biotin. Biotin can bind with high affinity to streptavidin or avidin. Cy5.5-PEG2000-Biotin plays an important role in the specific capture and detection of biomolecules.
    Cy5.5-PEG2000-Biotin
  • HY-W879342
    ICG-Tetrazine
    ICG-Tetrazine is a fluorescent dye.
    ICG-Tetrazine
  • HY-D2460B
    Dextran-CY5.5 (MW 40000)
    Dextran-CY5.5 (MW 40000) is a CY5.5 (HY-D0924) labeled Dextran (HY-112624) conjugate. CY5.5 is a Cyanine dye (The excitation wavelength is usually 673 nm and the emission wavelength is 707 nm) that can be used for the labeling of proteins, antibodies and small molecular compounds. Dextran has an inhibitory effect on thrombocyte aggregation and coagulation factors and is used as a plasma volume expander.
    Dextran-CY5.5 (MW 40000)
  • HY-NP0175
    Datura Stramonium Lectin (Biotinylated)
    Datura Stramonium Lectin (DSL，Biotinylated) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Datura Stramonium Lectin (DSL，Biotinylated) is a biological material or organic compound that can be used in life science research.
    Datura Stramonium Lectin (Biotinylated)
  • HY-D1442
    SBFI tetraammonium
    		98.0%
    SBFI (tetraammonium), the tetraammonium salt of SBFI, is a cell-impermeant sodium fluorescence indicator. SBFI (tetraammonium) is sodium-sensitive dye. The excitation wavelength is set 333 nm, and the emission wavelength is 539 nm for SBFI.
    SBFI tetraammonium
  • HY-D0015
    Cresol red, 97%
    		99.30%
    Cresol red, 97% is an acidic phenolsulfonphthalein triphenylmethane dye. Cresol red, 97% generates reactive oxygen species and singlet oxygen under ultrasonic irradiation, interacts with bovine serum albumin, alters the structure of bovine serum albumin, and oxidizes the fluorescent amino acid residues of bovine serum albumin. Cresol red, 97% can be decolorized and biodegraded by *Absidia spinosa* M15, producing phenylacetic acid and benzoic acid metabolites. Cresol red, 97% serves as a pH indicator (orange at pH 1.8-yellow at pH 2.0, yellow at pH 7.0-purple at pH 8.8). Cresol red, 97% can be used to indicate pH, temperature and salinity.
    Cresol red, 97%
  • HY-D2161
    AF 594 azide
    AF 594 azide is an azide derivative of the red fluorescent dye AF 594, which has high fluorescence quantum yield and high photostability (maximum absorption wavelength of 586 nm, maximum emission wavelength of 613 nm). AF 594 azide forms stable adducts by reaction of the azide group with alkynyl derivatives (terminal alkynes and cyclooctyne). It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    AF 594 azide
  • HY-N16309
    Cyanine 7 free acid bromide
    Cyanine 7 free acid bromide is a near-infrared fluorescent probe for labelling amine groups such as those on antibodies, nucleic acids, and proteins and can be detected using a variety of fluorescence detection techniques such as microscopy and flow cytometry.
    Cyanine 7 free acid bromide
Cat. No. 상품명 / Synonyms Application Reactivity