Cresol red, 97%
Based on 1 Customer Validation
Cresol red, 97% is an acidic phenolsulfonphthalein triphenylmethane dye. Cresol red, 97% generates reactive oxygen species and singlet oxygen under ultrasonic irradiation, interacts with bovine serum albumin, alters the structure of bovine serum albumin, and oxidizes the fluorescent amino acid residues of bovine serum albumin. Cresol red, 97% can be decolorized and biodegraded by *Absidia spinosa* M15, producing phenylacetic acid and benzoic acid metabolites. Cresol red, 97% serves as a pH indicator (orange at pH 1.8-yellow at pH 2.0, yellow at pH 7.0-purple at pH 8.8). Cresol red, 97% can be used to indicate pH, temperature and salinity.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.30%
- CAS 番号: 1733-12-6
- 分子式: C21H18O5S
- 分子量:382.43
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保管条件:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
体外実験
Cresol red, 97% binds tightly to bovine serum albumin (BSA) via a static quenching mechanism, with an equilibrium binding constant of 7.25×105 L/M. There is one primary binding site, which effectively quenches the intrinsic fluorescence of BSA[1].
Cresol red, 97% generates reactive oxygen species (ROS) under ultrasonic irradiation, with singlet oxygen (1O2) as the main component, as demonstrated by oxidative extraction spectrophotometry[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 1733-12-6
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性状 Solid
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分子量 382.43
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分子式 C21H18O5S
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SMILES
O=S1(OC(C2=CC=C(C(C)=C2)O)(C3=CC=CC=C31)C4=CC=C(C(C)=C4)O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
溶剤 & 溶解度
体外:
DMSO : 116.67 mg/mL (305.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
純度とドキュメンテーション
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データシート (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Zou M, et al. Sonodynamic and sonocatalytic damage of BSA molecules by Cresol Red, Cresol Red-DA and Cresol Red-DA-Fe under ultrasonic irradiation. Ultrason Sonochem. 2013 Mar;20(2):685-95. [Content Brief]
[2]. Kristanti RA, et al. Biotransformation studies of cresol red by Absidia spinosa M15. J Environ Manage. 2016;172:107-111. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6149 mL | 13.0743 mL | 26.1486 mL | 65.3714 mL |
| 5 mM | 0.5230 mL | 2.6149 mL | 5.2297 mL | 13.0743 mL | |
| 10 mM | 0.2615 mL | 1.3074 mL | 2.6149 mL | 6.5371 mL | |
| 15 mM | 0.1743 mL | 0.8716 mL | 1.7432 mL | 4.3581 mL | |
| 20 mM | 0.1307 mL | 0.6537 mL | 1.3074 mL | 3.2686 mL | |
| 25 mM | 0.1046 mL | 0.5230 mL | 1.0459 mL | 2.6149 mL | |
| 30 mM | 0.0872 mL | 0.4358 mL | 0.8716 mL | 2.1790 mL | |
| 40 mM | 0.0654 mL | 0.3269 mL | 0.6537 mL | 1.6343 mL | |
| 50 mM | 0.0523 mL | 0.2615 mL | 0.5230 mL | 1.3074 mL | |
| 60 mM | 0.0436 mL | 0.2179 mL | 0.4358 mL | 1.0895 mL | |
| 80 mM | 0.0327 mL | 0.1634 mL | 0.3269 mL | 0.8171 mL | |
| 100 mM | 0.0261 mL | 0.1307 mL | 0.2615 mL | 0.6537 mL |
Cresol red, 97% 関連分類
HY-D0015 関連分類
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