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  4. ATTO 488 maleimide

ATTO 488 maleimide is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 maleimide is a maleimide derivative of ATTO 488, which can be used to label proteins or antibodies.

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ATTO 488 maleimide

ATTO 488 maleimide Chemical Structure

CAS No. : 1219127-42-0

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Description

ATTO 488 maleimide is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 maleimide is a maleimide derivative of ATTO 488, which can be used to label proteins or antibodies.

Molecular Weight

825.74

Formula

C33H30F3N5O13S2
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

[NH2+]=C1C=CC2=C(C3=C(C(N(C)CCCC(NCCN4C(C=CC4=O)=O)=O)=O)C=CC=C3)C5=CC=C(N)C(S(O)(=O)=O)=C5OC2=C1S(O)(=O)=O.O=C([O-])C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (15.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2110 mL 6.0552 mL 12.1103 mL
5 mM 0.2422 mL 1.2110 mL 2.4221 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2110 mL 6.0552 mL 12.1103 mL 30.2759 mL
5 mM 0.2422 mL 1.2110 mL 2.4221 mL 6.0552 mL
10 mM 0.1211 mL 0.6055 mL 1.2110 mL 3.0276 mL
15 mM 0.0807 mL 0.4037 mL 0.8074 mL 2.0184 mL
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ATTO 488 maleimide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ATTO 488 maleimide1219127-42-0Fluorescent Dyefluorescent markerRhodamine structurestrong absorptionhigh fluorescence quantum yieldhigh thermal stabilityphotochemical stabilitysingle molecule detectionhigh-resolution microscopyInhibitorinhibitorinhibit

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