ATTO 488 maleimide
Based on 1 Customer Validation
ATTO 488 maleimide is a thiol-reactive fluorescent labeling reagent that forms a covalent bond via its maleimide group with the thiol group of the C-terminal cysteine residue of α-synGC. ATTO 488 maleimide enables α-syn fiber imaging via super-resolution stimulated emission depletion (STED) fluorescence microscopy. ATTO 488 maleimide is applicable to research related to Parkinson's disease (Ex/Em = 488/592nm).
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- Pureté : 96.64%
- CAS No.: 1219127-42-0
- Formule: C33H30F3N5O13S2
- Masse moléculaire:825.74
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
ATTO 488-maleimide performs site-specific labeling of C-terminal cysteine-modified α-synuclein, with a labeling efficiency of over 90%. The morphology of the resulting fibrils is comparable to that of unlabeled fibrils, and the AFM-STED colocalization ratio reaches as high as 0.84, indicating that nearly all fibrillar aggregates are fluorescent[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1219127-42-0
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Appearance Solid
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Masse moléculaire 825.74
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Formule C33H30F3N5O13S2
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Color Orange to red
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SMILES
[NH2+]=C1C=CC2=C(C3=C(C(N(C)CCCC(NCCN4C(C=CC4=O)=O)=O)=O)C=CC=C3)C5=CC=C(N)C(S(O)(=O)=O)=C5OC2=C1S(O)(=O)=O.O=C([O-])C(F)(F)F
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 12.5 mg/mL (15.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Human pluripotent stem cell midbrain dopaminergic neuron differentiation
Human pluripotent stem cells are directed toward midbrain dopaminergic neurons by first inducing a neural floor-plate-like progenitor state, then patterning cells with ventralizing SHH signaling and midbrain/WNT-FGF cues, and finally maturing progenitors into neurons expressing dopaminergic markers such as TH, NURR1/NR4A2, PITX3, DAT/SLC6A3, VMAT2/SLC18A2, GIRK2/KCNJ6, FOXA2, LMX1A, and EN1. The main readouts are loss of pluripotency, acquisition of FOXA2+/LMX1A+ midbrain floor-plate progenitors, emergence of βIII-tubulin+/MAP2+ neurons, and production of TH+ dopaminergic neurons with molecular, dopamine-release, and electrophysiological features of midbrain dopaminergic identity.
Pureté et documentation
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Fiche technique (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.2110 mL | 6.0552 mL | 12.1103 mL | 30.2759 mL |
| 5 mM | 0.2422 mL | 1.2110 mL | 2.4221 mL | 6.0552 mL | |
| 10 mM | 0.1211 mL | 0.6055 mL | 1.2110 mL | 3.0276 mL | |
| 15 mM | 0.0807 mL | 0.4037 mL | 0.8074 mL | 2.0184 mL |
ATTO 488 maleimide Related Classifications
HY-D2002 Related Classifications
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