2. Signaling Pathways
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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-177661C
    FAM labled Salanersen sodium
    FAM labled Salanersen sodiumis a FAM labled Salanersen sodium.
    FAM labled Salanersen sodium
  • HY-D2894
    4Arm-PEG2000-RB
    4Arm-PEG2000-RB (4Arm-PEG2000-Rhodamine B) is a fluorescent dye composed of Rhodamine B (HY-Y0016) and PEG with a four-arm structure. 4Arm-PEG2000-RB can be used for drug delivery and fluorescence imaging (Ex/Em=546/610 nm).
    4Arm-PEG2000-RB
  • HY-D1929
    ATTO 594 NHS ester
    ATTO 594 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 594 NHS ester is an NHS ester derivative of ATTO 594 that can be used to label proteins or antibodies.
    ATTO 594 NHS ester
  • HY-W716774
    5-TAMRA-Terazine
    5-TAMRA-Terazine is a fluorescent dye that combines 5-TAMRA (HY-15942) with a Terazine group. 5-TAMRA-Terazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups (Ex/Em = 541/567 nm).
    5-TAMRA-Terazine
  • HY-D1701
    Mag-Fura-2 AM
    Calcium is an important part of the human body, usually in the form of calcium, a large number of bones and teeth of the human body, a small amount of blood and tissues. MCE calcium ion detection probe can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence, in addition, Mag-indo-1/AM and Mag-Fluo-4 AM at a certain concentration (usually 5 mM) can effectively identify intracellular magnesium ions.
    Mag-Fura-2 AM
  • HY-D2930
    BG-SS-SulfoCy5
    BG-SS-SulfoCy5 (SNAP-SS-SulfoCy5) is a SulfoCy5-labeled SNAP tag fluorescent probe, linked by a disulfide bond. BG-SS-SulfoCy5 combines the specific recognition of SNAP-tag, the cleavability of disulfide bond, and the excellent optical properties of SulfoCy5. BG-SS-SulfoCy5 can be used for tracking the internalization of cell surface proteins and multiple labeling experiments.
    BG-SS-SulfoCy5
  • HY-131024
    Janelia Fluor® 549, Tetrazine
    Janelia Fluor® 549, Tetrazine (JF549, Tetrazine) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
    Janelia Fluor® 549, Tetrazine
  • HY-DY1097
    Thiazole Orange (solution)
    Thiazole Orange (solution) is an asymmetric anthocyanin dye that can be coupled with oligonucleotides (ONs) to prepare fluorescent hybridization probes. Thiazole Orange has been widely used in biomolecular detection and staining of DNA/ RNA in gels and can be used for reticulocyte analysis. Thiazole orange generates a significant fluorescence enhancement and high quantum yield when it binds with nucleic acids, especially RNA. Thiazole orange can permeate living cell membranes. Thiazole orange can use UV light for detection, but can also be detected with blue light. The excitation and emission of Thiazole orange are λex = 510 nm (488 nm and 470 nm also show strong excitation) and λem = 527 nm, respectively.
    Solvent and concentration: DMSO: 10 mM
    The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
    Thiazole Orange (solution)
  • HY-D2540
    Cy3-PEG10000-N3
    Cy3-PEG10000-N3 is a Cy3 (HY-D0822)-azide fluorescent dye used to label for protein and nucleic acid. Cy3-PEG10000-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cy3-PEG10000-N3
  • HY-D2802A
    FITC-PEG1000-N3
    FITC-PEG1000-N3 is an azide fluorescent dye containing polyethylene glycol (PEG) and FITC (HY-66019). FITC-PEG1000-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    FITC-PEG1000-N3
  • HY-N16327
    Squarefluor 631/646 free acid
    Squarefluor 631/646 free acid is a squaraine probe (Ex/Em = 631/646 nm).
    Squarefluor 631/646 free acid
  • HY-NP081
    Solanum Tuberosum (Potato) Lectin
    Solanum Tuberosum (Potato) Lectin (STL) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Solanum Tuberosum (Potato) Lectin (STL) is a biological material or organic compound that can be used in life science research.
    Solanum Tuberosum (Potato) Lectin
  • HY-D0968B
    Cy 3 (Non-Sulfonated) triethylamine
    Cy 3 Non-Sulfonated (Cyanine3) triethylamine is a cyanine (Cy) dye, and a fluorescent label with green channel for protein and nucleic acid. Cy 3 Non-Sulfonated triethylamine is a fluorescent photoproduct of Cyanine5 via photoconversion upon photoexcitation. Cy 3 Non-Sulfonated triethylamine can be used to high-density single-particle tracking in a living cell without using UV illumination and cell-toxic additives (Ex=470 nm; Em=515 nm and 565 nm nm).
    Cy 3 (Non-Sulfonated) triethylamine
  • HY-D2112
    TAMRA-PEG7-N3
    TAMRA-PEG7-N3 is a TAMRA (HY-135640) dye derivative containing 7 PEG units. TAMRA-PEG7-N3 undergoes copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing an alkynyl group (N3). It also undergoes strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
    TAMRA-PEG7-N3
  • HY-NP0131
    Griffonia (Bandeiraea) Simplicifolia Lectin I (Fluorescein)
    Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I) Fluorescein is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I) Fluorescein is a biological material or organic compound that can be used in life science research.
    Griffonia (Bandeiraea) Simplicifolia Lectin I (Fluorescein)
  • HY-NP164O
    Concanavalin A-Cy5
    Concanavalin A-Cy5 (Con A-Cy5) is a Cy5 (HY-D0821)-labled Concanavalin A (HY-P2149). Concanavalin A is a Ca2+/Mn2+-dependent and mannose/glucose-binding plant lectin. Concanavalin A binds the N- and O-glycosylated proteins of head and neck carcinoma cells glycoproteins (Ex/Em = 630/670 nm).
    Concanavalin A-Cy5
  • HY-NP089
    Soybean Agglutinin
    Soybean Agglutinin (SBA) is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Soybean Agglutinin (SBA) is a biological material or organic compound that can be used in life science research.
    Soybean Agglutinin
  • HY-D1545
    Violamine R
    Violamine R (Acid Violet 9) is a potent fluorophore. Violamine R shows environment and temperature-dependent photoluminescence (PL) intermittency in poly(vinyl alcohol) (PVOH) and potassium acid phthalate (KAP). Violamine R can be used to measure the temperatures spanning the glass-transition temperature (Tg) of the polymer host by the fluorescence intermittency or blinking in poly(vinyl alcohol) (PVA).
    Violamine R
  • HY-D1751
    RIM-1
    RIM-1 is a fluorescent probe for protein kinase C (PKC) that can be used to visualize the distribution of PKC in cells.
    RIM-1
  • HY-D1956
    ATTO 594 alkin
    ATTO 594 Alkin is an alkyne derivative of ATTO 594 with maximum excitation/emission wavelengths: 603/626 nm.
    ATTO 594 alkin
Cat. No. Product Name / Synonyms Application Reactivity