Janelia Fluor® 549, Tetrazine
Janelia Fluor? 549, Tetrazine (JF549, Tetrazine) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor? fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
For research use only. We do not sell to patients.
- Formula: C36H29N7O4
- Molecular Weight:623.66
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Janelia Fluor? fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as Tetrazine serve as reaction sites for covalent coupling—via chemical synthesis—to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
549
505
Chemical Information
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Molecular Weight 623.66
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Formula C36H29N7O4
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SMILES
O=C(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)NCC7=CC=C(C=C7)C8=NN=CN=N8
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Synonyms
JF549, Tetrazine
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)