PA Janelia Fluor® 549, SE
Based on 1 publication(s) in Google Scholar
PA Janelia Fluor 549, SE (PA-JF549-NHS) is a bright photoactivatable fluorophore of JF549,SE (JF549,NHS). JF549,SE (JF549,NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself.
For research use only. We do not sell to patients.
- Purity: 98.2%
- CAS No.: 1811539-42-0
- Formula: C32H25N5O6
- Molecular Weight:575.57
-
Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) PA Janelia Fluor® 549, SE
More
Biological Activity
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as NHS esters serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
PA Janelia Fluor is the integration of a four-member azetidine ring, which reduces the photobleaching and photodamage, improves the brightness and photostability of the fluorescent dye. PA Janelia Fluor exhibits cell membrane permeability that can be used for live cell imaging[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
549
505
Chemical Information
-
CAS No. 1811539-42-0
-
Appearance Solid
-
Molecular Weight 575.57
-
Formula C32H25N5O6
-
Color Light yellow to orange
-
SMILES
O=C(C1=CC=C(C(C23C4=CC=C(C=C4OC5=CC(N6CCC6)=CC=C35)N7CCC7)=C1)C(C2=[N+]=[N-])=O)ON8C(CCC8=O)=O
-
Synonyms
PA-JF549-NHS
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
-
Journal Impact Factor
-
Most Recent
Purity & Documentation
-
Data Sheet (279 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Jonathan B Grimm, et al. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol Biol. 2017;1663:179-188. [Content Brief]
[2]. Jonathan B Grimm, et al. Bright photoactivatable fluorophores for single-molecule imaging. Nat Methods. 2016 Dec;13(12):985-988. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PA Janelia Fluor® 549, SE Related Classifications
HY-133536 Related Classifications
Powered by Bioz