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Cat. No.: HY-138660

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HM Janelia Fluor® 526, SE

(Synonyms: HM-JF526 NHS)

HM Janelia Fluor® 526, SE (HM-JF526 NHS) is a derivative of hydroxymethyl JF526 (HM-JF526). SMLM (single-molecule localization microscopy) imaging in standard phosphate-buffered saline (pH 7.4) revealed that the HM-JF526 label showed spontaneous blinking behavior throughout the imaging session and did not require short-wavelength activation light. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

For research use only. We do not sell to patients.

CAS No.: 2376841-30-2
Formula: C₃₁H₂₁F₆N₃O₆
Molecular Weight:645.51

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

HM Janelia Fluor® 526, SE Chemical Structure

CAS No.: 2376841-30-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

HM Janelia Fluor® 526, SE (HM-JF526 NHS) is a derivative of hydroxymethyl JF526 (HM-JF526). SMLM (single-molecule localization microscopy) imaging in standard phosphate-buffered saline (pH 7.4) revealed that the HM-JF526 label showed spontaneous blinking behavior throughout the imaging session and did not require short-wavelength activation light^[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

Chemical Information

CAS No. 2376841-30-2
Molecular Weight 645.51
Formula C₃₁H₂₁F₆N₃O₆
SMILES

FC1=C(C=C2OC3=CC(N4CC(C4)(F)F)=C(F)C=C3C5(OCC6=CC=C(C=C56)C(ON7C(CCC7=O)=O)=O)C2=C1)N8CC(C8)(F)F
Synonyms

HM-JF526 NHS
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zheng Q, et al. Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging [published correction appears in ACS Cent Sci. 2020 Oct 28;6(10):1844]. ACS Cent Sci. 2019;5(9):1602-1613. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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