1811539-42-0
Chemical Structure
PA Janelia Fluor® 549, SE
Synonym(s): PA-JF549-NHS
- CAS No.: 1811539-42-0
- Formula:C32H25N5O6
- Molecular Weight:575.57
IUPAC Name: 2,5-dioxopyrrolidin-1-yl 3',6'-di(azetidin-1-yl)-2-diazo-3-oxo-2,3-dihydrospiro[indene-1,9'-xanthene]-6-carboxylate
InChIKey: ZJZWRNGNJDEPEQ-UHFFFAOYSA-N
SMILES: O=C(C1=CC=C(C(C23C4=CC=C(C=C4OC5=CC(N6CCC6)=CC=C35)N7CCC7)=C1)C(C2=[N+]=[N-])=O)ON8C(CCC8=O)=O
Biological Activity: PA Janelia Fluor 549, SE (PA-JF549-NHS) is a bright photoactivatable fluorophore of JF549,SE (JF549,NHS). JF549,SE (JF549,NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm[1][2]. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself.
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PA Janelia Fluor® 549, SE | 98.2% | PA Janelia Fluor 549, SE (PA-JF549-NHS) is a bright photoactivatable fluorophore of JF549,SE (JF549,NHS). JF549,SE (JF549,NHS) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. | ||||||||||||||||||||
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References
- [1]. Jonathan B Grimm, et al. Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol Biol. 2017;1663:179-188. [Content Brief]
- [2]. Jonathan B Grimm, et al. Bright photoactivatable fluorophores for single-molecule imaging. Nat Methods. 2016 Dec;13(12):985-988. [Content Brief]
