Janelia Fluor® 549, Azide
Based on 1 publication(s) in Google Scholar
Janelia Fluor? 549, Azide (JF549, Azide) is a fluorescent dye with the absorption maximum (λab (max)) of 549 nm and emission maximum (λem (max)) of 571 nm. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity: 99.49%
- Formula: C35H38N6O7
- Molecular Weight:654.71
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Janelia Fluor® 549, Azide
More
Biological Activity
571
549
Chemical Information
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Appearance Solid
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Molecular Weight 654.71
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Formula C35H38N6O7
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Color Purple to purplish red
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SMILES
O=C(C1=CC=C(C(C(C2=CC=C(C=C2OC3=C/4)N5CCC5)=C3C=CC4=[N+]6CCC/6)=C1)C([O-])=O)NCCOCCOCCOCCN=[N+]=[N-]
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Synonyms
JF549, Azide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
CRISPR live-cell imaging reveals chromatin dynamics and enhancer interactions at multiple non-repetitive loci. [Abstract]2025 Nov 6. PMID: 41199023
Solvent & Solubility
DMSO : 50 mg/mL (76.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5274 mL | 7.6370 mL | 15.2739 mL | 38.1848 mL |
| 5 mM | 0.3055 mL | 1.5274 mL | 3.0548 mL | 7.6370 mL | |
| 10 mM | 0.1527 mL | 0.7637 mL | 1.5274 mL | 3.8185 mL | |
| 15 mM | 0.1018 mL | 0.5091 mL | 1.0183 mL | 2.5457 mL | |
| 20 mM | 0.0764 mL | 0.3818 mL | 0.7637 mL | 1.9092 mL | |
| 25 mM | 0.0611 mL | 0.3055 mL | 0.6110 mL | 1.5274 mL | |
| 30 mM | 0.0509 mL | 0.2546 mL | 0.5091 mL | 1.2728 mL | |
| 40 mM | 0.0382 mL | 0.1909 mL | 0.3818 mL | 0.9546 mL | |
| 50 mM | 0.0305 mL | 0.1527 mL | 0.3055 mL | 0.7637 mL | |
| 60 mM | 0.0255 mL | 0.1273 mL | 0.2546 mL | 0.6364 mL |
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