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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2545
    Cy5-PEG10000-DSPE
    Cy5-PEG10000-DSPE is a PEG phospholipid with Cy5 (HY-D0821) dye used in protein/nucelic acid labeling and fluorescence microscopy. Cy5-PEG10000-DSPE can self-assemble in aqueous solution to form micelles/lipid bilayer and used to prepare liposomes or nanoparticles for nutrients delivery such as mRNA or DNA vaccine.
    Cy5-PEG10000-DSPE
  • HY-179073C
    TRITC-Polysucrose 400
    TRITC-Polysucrose 400 is TRITC-labeled Polysucrose 400 (HY-131960). TRITC-Polysucrose 400 is mainly used for permeability and microcirculation studies (Ex/Em = 544/570 nm).
    TRITC-Polysucrose 400
  • HY-D2842C
    FITC-PEG3400-biotin
    FITC-PEG3400-biotin is a fluorescent dye composed of polyethylene glycol (PEG), FITC (HY-66019) and a Biotin (HY-B0511). FITC-PEG3400-biotin can be used for fluorescent labeling and imaging.
    FITC-PEG3400-biotin
  • HY-D0676
    Acid yellow 199
    Acid yellow 199 is an azo dye that can be decolorized through reduction reaction.
    Acid yellow 199
  • HY-D2535
    Cy5-PEG5000-N3
    Cy5-PEG5000-N3 is a Cy5 (HY-D0821)-azide fluorescent dye used to label for protein and nucleic acid. Cy5-PEG5000-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Cy5-PEG5000-N3
  • HY-123612
    JAS239
    JAS239 is a blood-brain barrier-permeable ChoK inhibitor. JAS239 inhibits phosphocholine synthesis and reduces the expression level of E2F1 protein. JAS239 exhibits near-infrared fluorescence properties. JAS239 exerts anti-tumor activity against glioblastoma. JAS239 can be used in studies related to glioblastoma.
    JAS239
  • HY-150212D
    FAM labled Tilsotolimod sodium
    FAM labled Tilsotolimod sodiumis a FAM labled Tilsotolimod sodium.
    FAM labled Tilsotolimod sodium
  • HY-D1284
    BOP-JF646
    BOP-JF646 is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    BOP-JF646
  • HY-D1991
    ATTO 647
    ATTO 647 is a carborhodamine fluorophore and imaging tracer with photostable properties. ATTO 647 serves as a fluorescent probe to investigate cell membrane structure and diffusion characteristics. When conjugated with wheat germ agglutinin, ATTO 647 specifically binds to N-acetyl-β-D-glucosamine and sialic acid residues on membrane glycoproteins, enabling single-molecule tracing of glycoprotein diffusion. ATTO 647 exhibits highly stable fluorescence properties with significantly reduced blinking in mounting media such as ROXS (AA/MV) and ROXS (TX/TQ), whereas its brightness properties vary in Ibidi-MM and Vectashield. ATTO 647 can also be used to label histone H2B-GFP in fixed cells for confocal microscopy photobleaching experiments.
    ATTO 647
  • HY-D2802
    FITC-PEG400-N3
    FITC-PEG400-N3 is an azide fluorescent dye containing polyethylene glycol (PEG) and FITC (HY-66019). FITC-PEG400-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    FITC-PEG400-N3
  • HY-D3008
    ALP Green
    ALP Green (Probe 4) is an amphiphilic ALP fluorescent probe (Em = 550 nm). ALP Green can be used in the research of APAP (HY-66005)-induced liver injury.
    ALP Green
  • HY-D3282
    CCF1
    CCF1 is a carbon-rhodol-based turn-on fluorescent copper sensor with high selectivity for Cu+ over other biologically relevant metal ions. CCF1 detects changes in labile copper pools in living cells upon copper supplementation and/or depletion. CCF1 identifies elevations in labile copper pools in Atp7a-/- fibroblast cell models. CCF1 can be used for the research of Menkes disease.
    CCF1
  • HY-D2120
    Cy3-PEG3-endo-BCN
    Cy3-PEG3-endo-BCN is a dye derivative of Cyanine 3 (Cy3) (HY-D0822) containing 3 PEG units. Cy3-PEG3-endo-BCN contains the lyophilic bidentate macrocyclic ligand BCN, which can further synthesize macrocyclic complexes. In click chemistry, endo-BCN can react with molecules containing azide groups to form stable triazoles in the absence of catalysts.
    Cy3-PEG3-endo-BCN
  • HY-D3221
    MAO Probe 1
    MAO Probe 1 is a reactive two-photon fluorescent probe capable of crossing the blood-brain barrier, which is used to detect the activity of monoamine oxidases (MAO-A/MAO-B), with Km values of 70 μM (MAO-A) and 75 μM (MAO-B), respectively. IBC 2 (benzo[g]imino-coumarin 2), the enzymatic product of MAO Probe 1, can further specifically bind to and image Aβ plaques, enabling in vivo two-photon co-monitoring of MAO activity and Aβ plaques. MAO Probe 1 can be used in Alzheimer's disease research (IBC 2: Ex/Em = 850 nm/570-620 nm).
    MAO Probe 1
  • HY-D1499
    Fluorescent red 610
    Fluorescent red 610 is a fluorescent dye with an excitation peak at 590 nm and an emission peak at 610 nm.
    Fluorescent red 610
  • HY-D1930
    ATTO 425 azide
    ATTO 425 Azide is an azide derivative of ATTO 425, the maximum excitation and emission wavelength: 439/489 nm. ATTO 425 azide is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    ATTO 425 azide
  • HY-D1355
    Azide cyanine dye 728
    Azide cyanine dye 728 is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    Azide cyanine dye 728
  • HY-D2795B
    FITC-PEG5000-COOH
    FITC-PEG5000-COOH is a fluorescent dye composed of polyethylene glycol (PEG), FITC (HY-66019) and a COOH. FITC-PEG5000-COOH can be used for fluorescent labeling and imaging.
    FITC-PEG5000-COOH
  • HY-151774
    TAMRA hydrazide (6-isomer)
    TAMRA hydrazide (6-isomer) is a click chemistry reagent that can be coupled to carbonyl compounds. The dye hydrazide in TAMRA hydrazide (6-isomer) labels carbonyl compounds such as aldehydes and ketones. TAMRA in TAMRA hydrazide (6-isomer) is a xanthene red fluorophore that reacts with terminal alkynes.
    TAMRA hydrazide (6-isomer)
  • HY-D3334
    PE-CF594
    PE-CF594 is a labeled monoclonal antibody conjugate that specifically binds to HLA-DR on the surface of monocytes and B cells, while acting as a signal attenuator. Through steric hindrance and a possible fluorescence resonance energy transfer mechanism, PE-CF594 specifically reduces the fluorescence intensity of PE-CD124 staining, but does not interfere with the staining of other PE-labeled antibodies such as CD40, CD4 or CD14. PE-CF594 can also be used to detect the emission signal of mt-Keima after excitation with a 561-nm laser, thereby effectively evaluating mitophagy activity.
    PE-CF594
Cat. No. Product Name / Synonyms Application Reactivity