CCF1 

CCF1 is a carbon-rhodol-based turn-on fluorescent copper sensor with high selectivity for Cu⁺ over other biologically relevant metal ions. CCF1 detects changes in labile copper pools in living cells upon copper supplementation and/or depletion. CCF1 identifies elevations in labile copper pools in Atp7a^-/^- fibroblast cell models. CCF1 can be used for the research of Menkes disease^[1].

CCF1 is a fluorescent sensor with Cu⁺ selectivity in aqueous buffer at physiological pH, which produces a 5-fold fluorescence turn-on response with an apparent Cu⁺-binding K_d of 0.20 pM^[1].
CCF1 (2 μM; 15 min) detects increased and decreased levels of labile copper pools in live HEK 293T cells, with enhanced fluorescence under copper supplementation conditions and reduced fluorescence under copper depletion conditions^[1].
CCF1 (2 μM; 10 min) detects elevated levels of labile copper pools in live Atp7a^-/- MEFs relative to wild-type MEF controls^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

777.10

C₄₀H₅₁F₃N₂O₂S₄

2158208-42-3

CCSCCSCCN(CC1=CC=C(C(C(F)(F)F)=C1)C2=C3C=CC(C=C3C(C)(C4=C2C=CC(N5CCOCC5)=C4)C)=O)CCSCCSCC

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Jia S, et al. Tuning the Color Palette of Fluorescent Copper Sensors through Systematic Heteroatom Substitution at Rhodol Cores. ACS Chem Biol. 2018;13(7):1844-1852.  [Content Brief]