PE-CF594
Based on 1 Customer Validation
PE-CF594 is a labeled monoclonal antibody conjugate that specifically binds to HLA-DR on the surface of monocytes and B cells, while acting as a signal attenuator. Through steric hindrance and a possible fluorescence resonance energy transfer mechanism, PE-CF594 specifically reduces the fluorescence intensity of PE-CD124 staining, but does not interfere with the staining of other PE-labeled antibodies such as CD40, CD4 or CD14. PE-CF594 can also be used to detect the emission signal of mt-Keima after excitation with a 561-nm laser, thereby effectively evaluating mitophagy activity.
For research use only. We do not sell to patients.
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
PE-CF594 enables specific, sensitive detection of mt-Keima fluorescence in HeLa-Parkin cells stably expressing mt-Keima, supporting quantitative flow cytometric analysis of mitophagy activity[2].
PE-CF594 (0-25.0 μg/L; 3 min) can be quantified and discriminated from strongly overlapping fluorochromes using both compensated band pass filters and MOE-based optical computing, with MOE systems enabling multiplexed detection with two elements instead of three discrete filters[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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SMILES
[PE-CF594]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Purity & Documentation
References
[1]. De Vita M, et al. Unexpected interference in cell surface staining by monoclonal antibodies to unrelated antigens. Cytometry B Clin Cytom. Published online October 1, 2014. [Content Brief]
[2]. Um JH, et al. Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima. J Vis Exp. 2018;(138):58099. Published 2018 Aug 12. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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