Tween 80 (Synonyms: Polysorbate 80)

Name: Tween 80
Brand: MedChemExpress
SKU: HY-Y1891
Rating: 5.0 (110 reviews)

Cat. No.: HY-Y1891: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Tween 80 (Polysorbate 80), a surfactant, has been widely used as a solvent for pharmacological experiments. Tween 80 can also reduce bacterial attachment and inhibit biofilm formation.

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Tween 80 화학구조

CAS No. : 9005-65-6

사이즈	재고	수량
Solvent
50 mL	해외재고보유	Estimated Time of Arrival: December 31
Solvent
100 mL	해외재고보유	Estimated Time of Arrival: December 31
200 mL	견적 받기
500 mL	견적 받기

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 110 publication(s) in Google Scholar

110 Publications Citing Use of MCE Tween 80

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Biological Activity
순도&문서
References
고객리뷰

제품 설명

Tween 80 (Polysorbate 80), a surfactant, has been widely used as a solvent for pharmacological experiments. Tween 80 can also reduce bacterial attachment and inhibit biofilm formation.

In Vitro

Tween 80 (Polysorbate 80) is a surfactant which can also reduce bacterial attachment and inhibit biofilm formation^[1]. Solubility enhancement as high as 100-fold is achieved using 1% Tween 80 in vitro^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Tween 80 (Polysorbate 80) (IP; 10 ml/kg) significantly decreases locomotor activity at a concentration of 32% in saline (v/v) in male outbred (BALB/c x DBA/Z)F1 mice weighing 24-30 g^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

1309.63

화학식

C₆₄H₁₂₄O₂₆

CAS No.

9005-65-6

Appearance

Liquid (Density: 1.1 g/cm³)

Color

Colorless to light yellow

SMILES

[Tween 80]

선적

Room temperature in continental US; may vary elsewhere.

보관

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : ≥ 100 mg/mL (76.36 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (38.18 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.7636 mL	3.8179 mL	7.6357 mL
5 mM	0.1527 mL	0.7636 mL	1.5271 mL
10 mM	0.0764 mL	0.3818 mL	0.7636 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (1.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Select Batch:

Data Sheet (272 KB) SDS (394 KB)

COA (306 KB)

Handling Instructions (2659 KB)

References

[1]. Brandl MT, et al. Effect of the surfactant tween 80 on the detachment and dispersal of Salmonella enterica serovar Thompson single cells and aggregates from cilantro leaves as revealed by image analysis. Appl Environ Microbiol. 2014 Aug;80(16):5037-42. [Content Brief]

[2]. Pan Y, et al. Pharmacokinetics study of ferulic acid in rats after oral administration of γ-oryzanol under combined use of Tween 80 by LC/MS/MS. Eur Rev Med Pharmacol Sci. 2014;18(2):143-50. [Content Brief]

[3]. C A Castro, et al.Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6. [Content Brief]

[4]. Varma RK, Kaushal R, Junnarkar AY, et al. Polysorbate 80: a pharmacological study. Arzneimittelforschung. 1985;35(5):804-808. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	0.7636 mL	3.8179 mL	7.6357 mL	19.0894 mL
	5 mM	0.1527 mL	0.7636 mL	1.5271 mL	3.8179 mL
	10 mM	0.0764 mL	0.3818 mL	0.7636 mL	1.9089 mL
	15 mM	0.0509 mL	0.2545 mL	0.5090 mL	1.2726 mL
	20 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9545 mL
	25 mM	0.0305 mL	0.1527 mL	0.3054 mL	0.7636 mL
	30 mM	0.0255 mL	0.1273 mL	0.2545 mL	0.6363 mL
DMSO	40 mM	0.0191 mL	0.0954 mL	0.1909 mL	0.4772 mL
	50 mM	0.0153 mL	0.0764 mL	0.1527 mL	0.3818 mL
	60 mM	0.0127 mL	0.0636 mL	0.1273 mL	0.3182 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Tween 80 Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tween 809005-65-6Polysorbate 80Tween80Tween-80Polysorbate80Polysorbate-80Biochemical Assay ReagentsEnvironmental PollutantsEnvironmental ContaminantsInhibitorinhibitorinhibit

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Tween 80 (Synonyms: Polysorbate 80)

고객리뷰

Tween 80 Related Antibodies

110 Publications Citing Use of MCE Tween 80

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

순도&문서

References

고객리뷰

Tween 80 Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Tween 80 Related Classifications

Keywords:

최근 본 상품:

고객리뷰

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SAFETY DATA SHEET (SDS)

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