1. Metabolic Enzyme/Protease
  2. NADPH Oxidase
  3. VAS2870

VAS2870 

Cat. No.: HY-12804 Purity: >98.0%
Handling Instructions

VAS2870 is a NADPH oxidase (NOX) inhibitor.

For research use only. We do not sell to patients.

VAS2870 Chemical Structure

VAS2870 Chemical Structure

CAS No. : 722456-31-7

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 110 In-stock
Estimated Time of Arrival: December 31
25 mg USD 210 In-stock
Estimated Time of Arrival: December 31
50 mg USD 380 In-stock
Estimated Time of Arrival: December 31
100 mg USD 680 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    VAS2870 purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2020 Feb 1. pii: S0006-291X(20)30217-5.

    VAS2870 protects against LPS-induced cell injury through inhibiting Nox2 expression in A549 cells. The expression of Nox2 in control, LPS (150 mg/ml), VAS þ LPS (preincubating cells with 10 mM VAS2870 followed by 150 mg/ml LPS intervention) and VAS (10 mM VAS2870) groups is determined by western blot.

    VAS2870 purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2020 Feb 1. pii: S0006-291X(20)30217-5.

    VAS2870 protects against LPS-induced cell injury through inhibiting Nox2 expression in A549 cells. The expression of Nox2 in control, LPS (150 mg/ml), VAS þ LPS (preincubating cells with 10 mM VAS2870 followed by 150 mg/ml LPS intervention) and VAS (10 mM VAS2870) groups is determined by immunofluorescence labeling (E).
    • Biological Activity

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    • References

    • Customer Review

    Description

    VAS2870 is a NADPH oxidase (NOX) inhibitor.

    IC50 & Target

    Target: NADPH oxidase[1]

    In Vitro

    VAS2870 is effective to suppress PDGF-BB-dependent activation of NADPH oxidase and subsequent production of intracellular ROS. Furthermore, VAS2870 suppresses PDGF-BB-dependent chemotaxis, but not DNA synthesis. Preincubation with VAS2870 (10 and 20 μM) completely abolishes PDGF-mediated NADPH oxidase activation and ROS production. Preincubation with VAS2870 (0.1-20 μM) does not affect PDGF-induced cell cycle progression. However, it abolishes PDGF-dependent chemotaxis of VSMC in a concentration-dependent manner (100% inhibition at 10 μM)[1]. VAS2870 inhibits dose-dependently autocrine increase of cell number in FaO rat hepatoma cells, and almost completely blocked ROS production and thymidine incorporation when used at 25 mM. VAS2870 blocks serum-dependent cell growth of FaO rat hepatoma cells. VAS2870 inhibits proliferation of different human hepatocellular carcinoma (HCC) cell lines. VAS2870 pretreatment enhances TGF-b-mediated apoptosis of FaO rat hepatoma cells[2].

    Molecular Weight

    360.39

    Formula

    C₁₈H₁₂N₆OS

    CAS No.

    722456-31-7

    SMILES

    C1(N(CC2=CC=CC=C2)N=N3)=C3C(SC4=NC5=C(C=CC=C5)O4)=NC=N1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 83.3 mg/mL (231.14 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7748 mL 13.8739 mL 27.7477 mL
    5 mM 0.5550 mL 2.7748 mL 5.5495 mL
    10 mM 0.2775 mL 1.3874 mL 2.7748 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.94 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Kinase Assay
    [1]

    NADPH oxidase activity is measured by lucigenin-enhanced chemiluminescence in a 50 mM phosphate buffer (buffer A), pH 7.0, containing 1 mM EGTA, protease inhibitors, 150 mM sucrose, 5 μM lucigenin, and 250 μM NADPH as substrate. Quiescent cells are starved by serum deprivation for 24 h and treated as indicated, ished twice with ice-cold phosphate buffered saline (PBS), pH 7.4, and harvested. After low spin centrifugation, the pellet is re-suspended in ice-cold buffer A, lacking lucigenin and substrate. Then, the cells are lysed and total protein concentration is determined using a Bradford assay and adjusted to 1 mg/mL. 100 μL aliquots of the protein sample are measured over 6 min in quadruplicates using NADPH (100 μM) as substrate in a scintillation counter. Data are collected at 2 min intervals in order to measure relative changes in NADPH oxidase activity[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [2]

    To test autocrine growth, cells are serum deprived at 40% confluence and, when indicated, the NADPH oxidase inhibitors Apocynin (300 mM) or VAS2870 are added 30 min before serum deprivation and maintained along the experiment. For TGF-b experiments, cells at 70% confluence are serum deprived for 16 h and treated with 2 ng/mL TGF-β in the presence or absence of the EGFR inhibitor AG1478 (20 mM) or VAS2870 (25 mM), which are added 30 min prior to TGF-β[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    VAS2870VAS 2870VAS-2870NADPH OxidaseNOXInhibitorinhibitorinhibit

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    Product name:
    VAS2870
    Cat. No.:
    HY-12804
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