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  3. WEHI-345

WEHI-345 

Cat. No.: HY-18937 Purity: 99.27%
Handling Instructions

WEHI-345 is a potent and selective RIPK2 kinase inhibitor with an IC50 of 0.13 μM, which delays RIPK2 ubiquitylation and NF-κB activation on oligomerization domain (NOD) stimulation.

For research use only. We do not sell to patients.

WEHI-345 Chemical Structure

WEHI-345 Chemical Structure

CAS No. : 1354825-58-3

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 132 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 190 In-stock
Estimated Time of Arrival: December 31
50 mg USD 660 In-stock
Estimated Time of Arrival: December 31
100 mg USD 990 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    WEHI-345 purchased from MCE. Usage Cited in: Mol Cell. 2018 Feb 15;69(4):551-565.e7.

    THP1 cells are treated with muramyl dipeptide (MDP) for 30 min in the presence or absence of indicated kinase inhibitors (1 mM; added 1 hr prior) followed by lysis in 6 M urea buffer. Lysates are immunoprecipitated with K63 and linear ubiquitin chain antibodies, and precipitates and lysates are examined by western blotting using indicated antibodies.

    WEHI-345 purchased from MCE. Usage Cited in: J Immunol. 2017 May 1;198(9):3729-3736.

    Confocal images of lysozyme and procryptdin in cultured WT crypt organoids treated with GSK583 (5 μM) or mock-treated (A), or WEHI-345 (2.5 μM) or mock-treated (B) for 16 hours.
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    Description

    WEHI-345 is a potent and selective RIPK2 kinase inhibitor with an IC50 of 0.13 μM, which delays RIPK2 ubiquitylation and NF-κB activation on oligomerization domain (NOD) stimulation[1].

    IC50 & Target

    IC50: 0.13 μM (RIPK2 kinase)[1]

    In Vitro

    WEHI-345 (500 nM; Raw 267.4 cells) is able to inhibit MDP-induced autophosphorylation activity of RIPK2 in cells[1].
    WEHI-345 (500 nM; 0 hour, 2 hours, 4 hours, 8 hours; BMDMs or THP-1 cells) potently blocks MDP-induced transcription of the inflammatory mediators TNF and interleukin-6 (IL-6) in bone marrow-derived macrophages (BMDMs). In THP-1 cells, WEHI-345 reduces mRNA levels of NF-kB targets such as TNF, IL-8, IL-1b and A20[1].

    Western Blot Analysis[1]

    Cell Line: Raw 267.4 cells
    Concentration: 500 nM
    Incubation Time:
    Result: Inhibited MDP-induced autophosphorylation activity of RIPK2 in cells.

    RT-PCR[1]

    Cell Line: BMDMs or THP-1 cells
    Concentration: 500 nM
    Incubation Time: 0 hour, 2 hours, 4 hours, 8 hours
    Result: Blocked MDP-induced transcription of the inflammatory mediators TNF and interleukin-6 (IL-6) in BMDMs. And reduced mRNA levels of NF-kB targets in THP-1 cells.
    In Vivo

    WEHI-345 (20 mg/kg; intraperitoneal injection; twice daily; for 6 days; C57BL/6 male mice) treatment reduces disease score, inflammatory infiltrate, histological score and recruitment of dendritic cells to the site of inflammation. And improves body weight and reduces cytokine and chemokine levels, indicating an overall improvement of the condition in experimental autoimmune encephalomyelitis (EAE)-induced wild-type C57Bl/6 mice[1].

    Animal Model: C57BL/6 male mice (8-week-old)[1]
    Dosage: 20 mg/kg
    Administration: Intraperitoneal injection; twice daily; for 6 days
    Result: Reduced disease score, inflammatory infiltrate, histological score and recruitment of dendritic cells to the site of inflammation. And improved body weight and reduced cytokine and chemokine levels.
    Molecular Weight

    401.46

    Formula

    C₂₂H₂₃N₇O

    CAS No.

    1354825-58-3

    SMILES

    O=C(C1=CC=NC=C1)NCC(C)(N2N=C(C3=CC=C(C)C=C3)C4=C(N)N=CN=C42)C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (62.27 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4909 mL 12.4545 mL 24.9091 mL
    5 mM 0.4982 mL 2.4909 mL 4.9818 mL
    10 mM 0.2491 mL 1.2455 mL 2.4909 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.23 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.23 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    WEHI-345WEHI345WEHI 345RIP kinaseReceptor-interacting protein kinasesRIPKInhibitorinhibitorinhibit

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    Product name:
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    Cat. No.:
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