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  2. Acrylodan

Acrylodan 

Cat. No.: HY-W040230
Handling Instructions

Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34.

For research use only. We do not sell to patients.

Acrylodan Chemical Structure

Acrylodan Chemical Structure

CAS No. : 86636-92-2

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Description

Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M−1 cm−1 for actin at 290 nm, 18,500 M−1 cm−1 or acrylodan at 385 nm, and 71,000 M−1 cm−1 for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 (AcrylAct1NAT) is dramatically different than that of EDTA-unfolded AcrylAct1 (AcrylAct1I3), which has a very similar emission spectrum to CCT-bound AcrylAct1.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

225.29

Formula

C₁₅H₁₅NO

CAS No.

86636-92-2

SMILES

C=CC(C1=CC=C2C=C(C=CC2=C1)N(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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Keywords:

AcrylodanOthersChaperoneChaperoninProteinFoldingAMP-PNPPhosducin-likeInhibitorinhibitorinhibit

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Acrylodan
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HY-W040230
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