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  4. Acrylodan

Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34.

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Acrylodan Chemical Structure

Acrylodan Chemical Structure

CAS No. : 86636-92-2

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10 mg USD 128 In-stock
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Based on 1 publication(s) in Google Scholar

Acrylodan Related Antibodies

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Description

Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M 1 cm 1 for actin at 290 nm, 18,500 M 1 cm 1 or acrylodan at 385 nm, and 71,000 M 1 cm 1 for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 (AcrylAct1NAT) is dramatically different than that of EDTA-unfolded AcrylAct1 (AcrylAct1I3), which has a very similar emission spectrum to CCT-bound AcrylAct1.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Acrylodan Related Antibodies
Molecular Weight

225.29

Formula

C15H15NO
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

C=CC(C1=CC=C2C=C(C=CC2=C1)N(C)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (221.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4387 mL 22.1936 mL 44.3872 mL
5 mM 0.8877 mL 4.4387 mL 8.8774 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 95.01%

COA (245 KB) HNMR (219 KB) LCMS (266 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.4387 mL 22.1936 mL 44.3872 mL 110.9681 mL
5 mM 0.8877 mL 4.4387 mL 8.8774 mL 22.1936 mL
10 mM 0.4439 mL 2.2194 mL 4.4387 mL 11.0968 mL
15 mM 0.2959 mL 1.4796 mL 2.9591 mL 7.3979 mL
20 mM 0.2219 mL 1.1097 mL 2.2194 mL 5.5484 mL
25 mM 0.1775 mL 0.8877 mL 1.7755 mL 4.4387 mL
30 mM 0.1480 mL 0.7398 mL 1.4796 mL 3.6989 mL
40 mM 0.1110 mL 0.5548 mL 1.1097 mL 2.7742 mL
50 mM 0.0888 mL 0.4439 mL 0.8877 mL 2.2194 mL
60 mM 0.0740 mL 0.3699 mL 0.7398 mL 1.8495 mL
80 mM 0.0555 mL 0.2774 mL 0.5548 mL 1.3871 mL
100 mM 0.0444 mL 0.2219 mL 0.4439 mL 1.1097 mL
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Acrylodan Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Acrylodan86636-92-2Fluorescent DyeChaperoneChaperoninProteinFoldingAMP-PNPPhosducin-likeInhibitorinhibitorinhibit

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