Acrylodan
Based on 1 Customer Validation
Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34.
For research use only. We do not sell to patients.
- Purity: 95.05%
- CAS No.: 86636-92-2
- Formula: C15H15NO
- Molecular Weight:225.29
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Purification and Labeling of Act1:
1. 20-fold molar excess of Acrylodan was added to the polymerized actin and incubated on ice overnight.
2. The labeled actin is centrifuged (2 h, 4°C, 100,000 g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4°C.
3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M 1 cm 1 for actin at 290 nm, 18,500 M 1 cm 1 or acrylodan at 385 nm, and 71,000 M 1 cm 1 for Alexa Fluor 488 at 494 nm.
4. Acrylodan-labeled Act1 (AcrylAct1NAT) is dramatically different than that of EDTA-unfolded AcrylAct1 (AcrylAct1I3), which has a very similar emission spectrum to CCT-bound AcrylAct1.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 86636-92-2
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Appearance Solid
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Molecular Weight 225.29
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Formula C15H15NO
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Color Light yellow to yellow
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SMILES
C=CC(C1=CC=C2C=C(C=CC2=C1)N(C)C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (221.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Sarah F Stuart, et al. A two-step mechanism for the folding of actin by the yeast cytosolic chaperonin. J Biol Chem. 2011 Jan 7;286(1):178-84. [Content Brief]
[2]. K Flora, et al. Unfolding of acrylodan-labeled human serum albumin probed by steady-state and time-resolved fluorescence methods. Biophys J. 1998 Aug;75(2):1084-96. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.4387 mL | 22.1936 mL | 44.3872 mL | 110.9681 mL |
| 5 mM | 0.8877 mL | 4.4387 mL | 8.8774 mL | 22.1936 mL | |
| 10 mM | 0.4439 mL | 2.2194 mL | 4.4387 mL | 11.0968 mL | |
| 15 mM | 0.2959 mL | 1.4796 mL | 2.9591 mL | 7.3979 mL | |
| 20 mM | 0.2219 mL | 1.1097 mL | 2.2194 mL | 5.5484 mL | |
| 25 mM | 0.1775 mL | 0.8877 mL | 1.7755 mL | 4.4387 mL | |
| 30 mM | 0.1480 mL | 0.7398 mL | 1.4796 mL | 3.6989 mL | |
| 40 mM | 0.1110 mL | 0.5548 mL | 1.1097 mL | 2.7742 mL | |
| 50 mM | 0.0888 mL | 0.4439 mL | 0.8877 mL | 2.2194 mL | |
| 60 mM | 0.0740 mL | 0.3699 mL | 0.7398 mL | 1.8495 mL | |
| 80 mM | 0.0555 mL | 0.2774 mL | 0.5548 mL | 1.3871 mL | |
| 100 mM | 0.0444 mL | 0.2219 mL | 0.4439 mL | 1.1097 mL |
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