2. ADU-S100 disodium salt

ADU-S100 disodium salt  (Synonyms: MIW815 disodium salt; ML RR-S2 CDA disodium salt)

Cat. No.: HY-12885A Purity: 99.62%
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ADU-S100 disodium salt (MIW815 disodium salt) is an activator of stimulator of interferon genes (STING).

For research use only. We do not sell to patients.

ADU-S100 disodium salt Chemical Structure

ADU-S100 disodium salt Chemical Structure

CAS No. : 1638750-95-4

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1 mg USD 195 In-stock
5 mg USD 645 In-stock
10 mg USD 1150 In-stock
25 mg USD 2400 In-stock
50 mg USD 4200 In-stock
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Customer Review

Based on 42 publication(s) in Google Scholar

Other Forms of ADU-S100 disodium salt:

ADU-S100 disodium salt Related Antibodies

Top Publications Citing Use of Products

41 Publications Citing Use of MCE ADU-S100 disodium salt

RT-PCR

    ADU-S100 disodium salt purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 13;14(1):1390.  [Abstract]

    ADU-S100 (10 μM; 48 h; labeled as STING) enhances type I interferon gene expression in human dendritic cells (DCs).

    • Biological Activity

    • Protocol

    • Purity & Documentation

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    Description

    ADU-S100 disodium salt (MIW815 disodium salt) is an activator of stimulator of interferon genes (STING).

    IC50 & Target

    STING[1]
    In Vitro

    ADU-S100 shows enhanced type I IFN production over CDA in THP-1 human monocytes. In contrast, the dithio, mixed-linkage cyclic dinucleotide (CDN) derivatives (ML RR-CDA, ML RR-S2 CDG, and ML RR-S2 cGAMP) potently activate all five hSTING alleles, including the refractory hSTINGREF and hSTINGQ alleles. ADU-S100 induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. ADU-S100 is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). ADU-S100 induces significantly higher levels of IFN-α when compared to ML cGAMP[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ADU-S100 disodium salt Related Antibodies
    In Vivo

    ADU-S100 shows higher anti-tumor control than the endogenous ML cGAMP. A dose response of the ADU-S100 compound is performed in B16 tumor-bearing mice, which identifies an optimal antitumor dose level that also elicites maximum tumor antigen-specific CD8+ T cell responses, and improves long-term survival to 50%[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    Molecular Weight

    734.51

    Formula

    C20H22N10Na2O10P2S2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC([C@](CO[P@]1(S[Na])=O)([H])O2)([H])[C@](O[P@@](OC[C@](O[C@@H](N3C4=NC=NC(N)=C4N=C3)[C@@H]5O)([H])[C@@]5([H])O1)(S[Na])=O)([H])[C@@H]2N6C7=NC=NC(N)=C7N=C6
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (136.15 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3615 mL 6.8073 mL 13.6145 mL
    5 mM 0.2723 mL 1.3615 mL 2.7229 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    Concentration (final)

    C2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: ≥ 25 mg/mL (34.04 mM); Clear solution

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.91%

    COA (251 KB) HNMR (250 KB) CNMR (196 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    Cell Assay
    [1]

    Cryopreserved hPBMCs are thawed and 1×106 cells per well are plated in a 96 well plate in RPMI media supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin, L-glutamine, 10 mM HEPES buffer, 1 mM Sodium Pyruvate, 0.055 mM β-ME at 37°C with 5% CO2. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer and analyzed by FCAP Array Software[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice[1]
    WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm3 they received three IT doses of ADU-S100 at 5, 25, 50 or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17 and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.3615 mL 6.8073 mL 13.6145 mL 34.0363 mL
    5 mM 0.2723 mL 1.3615 mL 2.7229 mL 6.8073 mL
    10 mM 0.1361 mL 0.6807 mL 1.3615 mL 3.4036 mL
    15 mM 0.0908 mL 0.4538 mL 0.9076 mL 2.2691 mL
    20 mM 0.0681 mL 0.3404 mL 0.6807 mL 1.7018 mL
    25 mM 0.0545 mL 0.2723 mL 0.5446 mL 1.3615 mL
    30 mM 0.0454 mL 0.2269 mL 0.4538 mL 1.1345 mL
    40 mM 0.0340 mL 0.1702 mL 0.3404 mL 0.8509 mL
    50 mM 0.0272 mL 0.1361 mL 0.2723 mL 0.6807 mL
    60 mM 0.0227 mL 0.1135 mL 0.2269 mL 0.5673 mL
    80 mM 0.0170 mL 0.0851 mL 0.1702 mL 0.4255 mL
    100 mM 0.0136 mL 0.0681 mL 0.1361 mL 0.3404 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ADU-S100 disodium1638750-95-4MIW815 disodiumML RR-S2 CDA disodiumInhibitorinhibitorinhibit

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