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PGRMC1 Antibody (YA3488)

(Synonyms: MPR; HPR6.6)
Customer Review

Based on 1 Customer Validation

PGRMC1 Antibody (YA3488) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PGRMC1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL IHC-P FC ICC
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • PGRMC1 Antibody (YA3488) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

PGRMC1 Antibody (YA3488) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PGRMC1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 22 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 22 kDa
Immunogen

Purified recombinant fragment of human PGRMC1 (AA:1-195) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718766

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human prostate tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Flow cytometric analysis of 1X10^6 A549 cells labeling PGRMC1 Antibody(HY-P83791
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunofluorescence analysis of Hela cells labeling PGRMC1 antibody (HY-P83791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PGRMC1 component of a progesterone-binding protein complex. Binds progesterone. Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan. Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins. Forms a ternary complex with TMEM97 receptor and low density lipid receptor/LDLR, which increases LDLR-mediated LDL lipoprotein internalization[1][2][3][4].

  • Subcellular Localization

    Microsome membrane; Single-pass membrane protein; Smooth endoplasmic reticulum membrane; Single-pass membrane protein; Mitochondrion outer membrane; Single-pass membrane protein; Extracellular side; Secreted

  • Expression


    Tissue_specificity:This protein can be detected in urine (protein level) (PubMed:36213313, PubMed:37453717) . It is expressed in the endometrial glands and stroma (protein level) (PubMed:23793472) . This protein is widely expressed, with the highest expression levels in the liver and kidneys.

  • Subunit

    Homodimer. Forms stable homodimer through hydrophobic heme-heme stacking interactions (PubMed:26988023, PubMed:28396637). Interacts with FECH; the interaction results in decreased FECH activity (PubMed:27599036).

  • SwissProt ID

    O00264

  • Gene ID
    10857 [NCBI]
  • Synonyms

    MPR; HPR6.6

PGRMC1 Antibody (YA3488) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling PGRMC1 Antibody(HY-P83791

  • 1/16

    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling PGRMC1 antibody (HY-P83791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGRMC1 Antibody (YA3488) Product Image
    1/16
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • PGRMC1 Antibody (YA3488) Product Image
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human prostate tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human kidney tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling PGRMC1 Antibody(HY-P83791
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunofluorescence analysis of Hela cells labeling PGRMC1 antibody (HY-P83791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • Experimental Validation Results for PGRMC1 Antibody (YA3488)
  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using PGRMC1 Antibody (HY-P83791, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human epididymis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human prostate tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using PGRMC1 Antibody (HY-P83791, 1/800). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 A549 cells labeling PGRMC1 Antibody(HY-P83791

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunofluorescence analysis of Hela cells labeling PGRMC1 antibody (HY-P83791) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGRMC1 Antibody (YA3488) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PGRMC1 with PGRMC1 Antibody (HY-P83791) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PGRMC1 Antibody (HY-P83791)) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image IHC-P
  • PGRMC1 Antibody (YA3488) Product Image FC
  • PGRMC1 Antibody (YA3488) Product Image ICC
  • PGRMC1 Antibody (YA3488) Product Image ICC
  • PGRMC1 Antibody (YA3488) Product Image ICC