Antiviral agent 80 

Antiviral agent 80 is a conjugate of Zanamivir (HY-13210) and Amantadine (HY-13317), acting as a dual inhibitor of influenza virus M2 ion channel/neuraminidase (NA). Antiviral agent 80 exhibits potent inhibitory activity against both wild-type and drug-resistant influenza neuraminidase mutants, with an IC₅₀ value range of 1.50 nM to 120.4 nM. Antiviral agent 80 can be used in influenza-related research^[1].

Antiviral agent 80 (Compound 7j) exhibits potent inhibitory activity against both wild-type and drug-resistant influenza neuraminidase (NA) mutants, with IC₅₀ values ranging from 1.50 nM to 120.4 nM. Specifically, its IC₅₀ values against N9, N1, and N1 (H275Y) are 1.5 nM, 1.69 nM, and 2.05 nM, respectively^[1].
Antiviral agent 80 protects MDCK cells from influenza A virus-induced cytopathic effects, with an EC₅₀ value ranging from 11.13 nM to 12.55 nM and a CC₅₀ greater than 200 μM, indicating high selectivity^[1].
Antiviral agent 80 (5 μM; 24 h) induces proteasome-dependent NA degradation in HEK293T cells transfected with NA plasmid, with a degradation rate of approximately 95%; it does not upregulate autophagy markers in BEAS-2B, HEK293T or A549 cells^[1].
Antiviral agent 80 can efficiently cross cell membranes and accumulate in MDCK cells, with intracellular retention rates of 88.8% and 19.1% at pretreatment concentrations of 100 μM and 25 μM, respectively; after removing extracellular compounds by washing, its EC₅₀ against A/WSN/1933 only slightly increases from 11.13 nM to 48.0 nM, indicating that this compound possesses strong intracellular antiviral activity^[1].
Antiviral agent 80 inhibits multiple stages of the influenza A virus life cycle in MDCK cells, which is evidenced by the reduction in M1 protein levels^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

580.63

C₂₆H₄₀N₆O₉

3110745-57-5

CC(N[C@H]1[C@H]([C@H](OC(NCCNC(C23CC4CC(C3)CC(C4)C2)=O)=O)[C@H](O)CO)OC(C(O)=O)=C[C@@H]1NC(N)=N)=O

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Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wang Y, et al. Zanamivir-Amantadine Conjugate: A Dual-Action Agent with Broad-Spectrum Synergistic Antiviral Efficacy. J Med Chem. 2026;69(5):6063-6083.  [Content Brief]