ATTO 488 maleimide 

ATTO 488 maleimide is a thiol-reactive fluorescent labeling reagent that forms a covalent bond via its maleimide group with the thiol group of the C-terminal cysteine residue of α-synGC. ATTO 488 maleimide enables α-syn fiber imaging via super-resolution stimulated emission depletion (STED) fluorescence microscopy. ATTO 488 maleimide is applicable to research related to Parkinson's disease (Ex/Em = 488/592nm)^[1].

ATTO 488-maleimide performs site-specific labeling of C-terminal cysteine-modified α-synuclein, with a labeling efficiency of over 90%. The morphology of the resulting fibrils is comparable to that of unlabeled fibrils, and the AFM-STED colocalization ratio reaches as high as 0.84, indicating that nearly all fibrillar aggregates are fluorescent^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

825.74

C₃₃H₃₀F₃N₅O₁₃S₂

1219127-42-0

Solid

Orange to red

[NH2+]=C1C=CC2=C(C3=C(C(N(C)CCCC(NCCN4C(C=CC4=O)=O)=O)=O)C=CC=C3)C5=CC=C(N)C(S(O)(=O)=O)=C5OC2=C1S(O)(=O)=O.O=C([O-])C(F)(F)F

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Jadavi S, et al. Fluorescence labeling methods influence the aggregation process of α-syn in vitro differently. Nanoscale. 2023;15(18):8270-8277. Published 2023 May 11.  [Content Brief]