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  3. Biochemical Assay Reagents
  4. Bis-Tris

Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5).

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Bis-Tris

Bis-Tris Chemische Struktur

CAS. Nr. : 6976-37-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Other Forms of Bis-Tris:

Bis-Tris Related Antibodies

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Beschreibung

Bis-Tris is an amine buffer suitable for protein and nucleic acid systems with a pH buffer range of 5.8-7.2. Bis-Tris can also be mixed with HEPES and cacodylic acid buffer to create a pH 8 environment and monitor fluorescence emission intensity at 305 nm. Gels formulated with Bis-Tris also avoid protein degradation in samples prepared at higher pH (pH 8.5)[1][2][3].

In Vitro

Bis-Tris (10 mM or 4-12% Bis-Tris gel) has excellent buffering capacity and, in synergy with HEPES and cacodylic acid, covers a wide pH range. It can be used for fluorescence spectroscopy analysis, circular dichroism (CD) analysis, GST pull-down binding assay, gel filtration analysis, nuclear magnetic resonance (NMR) analysis, and protein electrophoresis. Abnormal pH fluctuations can affect experimental results[2][3].
Buffers containing Bis-Tris can be used to preserve purified proteins to prevent degradation (4°C)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bis-Tris Related Antibodies
Molekulargewicht

209.24

Formel

C8H19NO5
CAS. Nr.
Appearance

Solid

Color

White to off-white

SMILES

OCC(CO)(N(CCO)CCO)CO
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Store at room temperature, keep dry and cool

In solvent -80°C 1 year
-20°C 6 months
Lösungsmittel & Löslichkeit
In Vitro: 

H2O : 200 mg/mL (955.84 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.7792 mL 23.8960 mL 47.7920 mL
5 mM 0.9558 mL 4.7792 mL 9.5584 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (238.96 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Reinheit & Dokumentation

Purity: 98.0%

SDS (476 KB)

COA (247 KB) HNMR (115 KB) MS (191 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.7792 mL 23.8960 mL 47.7920 mL 119.4800 mL
5 mM 0.9558 mL 4.7792 mL 9.5584 mL 23.8960 mL
10 mM 0.4779 mL 2.3896 mL 4.7792 mL 11.9480 mL
15 mM 0.3186 mL 1.5931 mL 3.1861 mL 7.9653 mL
20 mM 0.2390 mL 1.1948 mL 2.3896 mL 5.9740 mL
25 mM 0.1912 mL 0.9558 mL 1.9117 mL 4.7792 mL
30 mM 0.1593 mL 0.7965 mL 1.5931 mL 3.9827 mL
40 mM 0.1195 mL 0.5974 mL 1.1948 mL 2.9870 mL
50 mM 0.0956 mL 0.4779 mL 0.9558 mL 2.3896 mL
60 mM 0.0797 mL 0.3983 mL 0.7965 mL 1.9913 mL
80 mM 0.0597 mL 0.2987 mL 0.5974 mL 1.4935 mL
100 mM 0.0478 mL 0.2390 mL 0.4779 mL 1.1948 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Bis-Tris Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bis-Tris6976-37-0Biochemical Assay ReagentsProtein degradationamine buffersInhibitorinhibitorinhibit

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